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The map label for this gene is rlmH

Identifier: 85058776

GI number: 85058776

Start: 1352293

End: 1352763

Strand: Reverse

Name: rlmH

Synonym: SG0798

Alternate gene names: 85058776

Gene position: 1352763-1352293 (Counterclockwise)

Preceding gene: 85058777

Following gene: 85058775

Centisome position: 32.43

GC content: 59.87

Gene sequence:

>471_bases
GTGAGACTGCAGCTTATCGCCATCGGCACCAAAATGCCGGACTGGGTGCAGACCGGGTTTAGCGACTATCTTAAGCGCTT
TCCCAAAGATTTGCCCTTCGACCTGACGGAAATTGCGGCCGGTAAGCGCGGCAAAAACGCGGATATTGCACGTATCCTGG
ACAAGGAAGGCGAGCAGATGCTAGCAGCGGTAGCAAAAGGGAACCGCATTGTTACGCTGGACATCCCCGGCGCGCGCTGG
GAAACACCGCGGCTGGCGCAGCAGCTCGAGCGCTGGAAACAAGACGGGCGCGATGTCAGTTTCCTTATCGGCGGGCCCGA
GGGGCTGGCCCCTGCCTGCAAGGCAGGGGCCGAGCAAAGCTGGTCGCTGTCCCCGTTAACTTTACCGCATCCGCTGGTCC
GGGTGCTGGTCGCGGAGAGCCTGTATCGTGCGTGGAGCATTACAACCAATCACCCTTATCACCGTGAGTAA

Upstream 100 bases:

>100_bases
CGCCGACTGGATCGTGGTCGACCTGGGTGATGTGATTGTGCATGTGATGCAGGAAGAAAGCCGCCGTCTGTATGAGCTGG
AAAAGCTCTGGAGCTGAGGG

Downstream 100 bases:

>100_bases
CGCGGCGACGATGATTAACGTGAACTAATTCCGCTGAATGAAAATAGAACGTAACCCCTTTCGCGACTATTCAGCCGAGT
CGGCGCTATTTGTGCGTCGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRLQLIAIGTKMPDWVQTGFSDYLKRFPKDLPFDLTEIAAGKRGKNADIARILDKEGEQMLAAVAKGNRIVTLDIPGARW
ETPRLAQQLERWKQDGRDVSFLIGGPEGLAPACKAGAEQSWSLSPLTLPHPLVRVLVAESLYRAWSITTNHPYHRE

Sequences:

>Translated_156_residues
MRLQLIAIGTKMPDWVQTGFSDYLKRFPKDLPFDLTEIAAGKRGKNADIARILDKEGEQMLAAVAKGNRIVTLDIPGARW
ETPRLAQQLERWKQDGRDVSFLIGGPEGLAPACKAGAEQSWSLSPLTLPHPLVRVLVAESLYRAWSITTNHPYHRE
>Mature_156_residues
MRLQLIAIGTKMPDWVQTGFSDYLKRFPKDLPFDLTEIAAGKRGKNADIARILDKEGEQMLAAVAKGNRIVTLDIPGARW
ETPRLAQQLERWKQDGRDVSFLIGGPEGLAPACKAGAEQSWSLSPLTLPHPLVRVLVAESLYRAWSITTNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=83.974358974359, Blast_Score=274, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_SODGM (Q2NUV2)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454478.1
- ProteinModelPortal:   Q2NUV2
- SMR:   Q2NUV2
- STRING:   Q2NUV2
- GeneID:   3868481
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0798
- NMPDR:   fig|343509.6.peg.1722
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   Q2NUV2
- ProtClustDB:   PRK00103
- BioCyc:   SGLO343509:SG0798-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17458; Mature: 17458

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQLIAIGTKMPDWVQTGFSDYLKRFPKDLPFDLTEIAAGKRGKNADIARILDKEGEQM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
LAAVAKGNRIVTLDIPGARWETPRLAQQLERWKQDGRDVSFLIGGPEGLAPACKAGAEQS
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCC
WSLSPLTLPHPLVRVLVAESLYRAWSITTNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRLQLIAIGTKMPDWVQTGFSDYLKRFPKDLPFDLTEIAAGKRGKNADIARILDKEGEQM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
LAAVAKGNRIVTLDIPGARWETPRLAQQLERWKQDGRDVSFLIGGPEGLAPACKAGAEQS
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCC
WSLSPLTLPHPLVRVLVAESLYRAWSITTNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA