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The map label for this gene is lipA

Identifier: 85058770

GI number: 85058770

Start: 1345052

End: 1346017

Strand: Reverse

Name: lipA

Synonym: SG0792

Alternate gene names: 85058770

Gene position: 1346017-1345052 (Counterclockwise)

Preceding gene: 85058771

Following gene: 85058759

Centisome position: 32.27

GC content: 56.83

Gene sequence:

>966_bases
ATGAGTAAACCGATACAGATGGAACGCGGCGTTAAATACCGTGACGCTGATAAAATGGCGTTAATCCCTATCAAAACCGT
CGCGGTGGAACGTCAGGAAATTTTGCGCAAGCCCTCCTGGATGAAAATCAAATTGCCCGCCGACTCGACCCGTATTCAGG
GCATCAAGGTGGCAATGCGTAAAAACGGCCTGCACTCGGTGTGCGAGGAAGCCTCCTGCCCTAATTTGGCGGAATGCTTT
AACCACGGCACCGCCACCTTTATGATTCTGGGGGCCATCTGCACCCGGCGTTGCCCTTTCTGCGATGTCGCGCACGGCCG
TCCCGTCACCCCGGATGCCAACGAGCCGGAGAAACTGGCGCAAACCATCGCCGACATGGGGCTGCGCTACGTCGTGGTCA
CCTCTGTCGATCGCGACGACCTGCGCGATGGCGGCGCCCAGCATTTCGCCGACTGTATCAGTGCCATCCGCGCCAAAAAT
CCTAACATCCGCATCGAGACGCTGGTGCCCGACTTCCGAGGTCGTATGGATCGCGCGCTGGAAATCATTAACGCGGCGCC
GCCCGATGTCTTTAACCATAATTTGGAAAACGTGCCGCGTCTGTATCGTCAGGTGCGTCCCGGCGCCGACTATCATTGGT
CGCTCAAGCTGCTGGAAAACTTCAAAGTCGCCAATCCGCAGTTGCCGACCAAATCCGGTTTGATGGTGGGATTGGGCGAA
ACCAACGCCGAAATCGTTGATGTGATGCGCGACCTGTGTCGTCATGGCGTCACCATGTTGACGCTGGGCCAGTATTTGCA
GCCCAGCCGCCATCACCTGCCCGTTAAGCGCTATGTCAGTCCGCAAGAATTTGATGAGATGAAACAAGAAGCGTTGGCGA
TGGGCTTCACCCACGCCGCCTGCGGGCCCTTCGTACGCTCGTCCTACCACGCGGATTTGCAGGCTAAAGGGATAGAAGTC
AAATAA

Upstream 100 bases:

>100_bases
ATAATTTTTAAAATTTTTTTGATATTCGCCAAAAATTCACGCAACAGACTGATTTATCTGCTTTTCAGGCTGTCGACTTC
TGGACTGGACCTGCACCATT

Downstream 100 bases:

>100_bases
TCTCCCGGCGGGCGGGCAAAAAAAGCGGACGCCTCAGGTGTCCGCCTTTTTGCCGCGATGCATTATTCTTTGCGATCGGC
GATGCGCGCTGCATCATCAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIQMERGVKYRDADKMALIPIKTVAVERQEILRKPSWMKIKLPADSTRIQGIKVAMRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLAQTIADMGLRYVVVTSVDRDDLRDGGAQHFADCISAIRAKN
PNIRIETLVPDFRGRMDRALEIINAAPPDVFNHNLENVPRLYRQVRPGADYHWSLKLLENFKVANPQLPTKSGLMVGLGE
TNAEIVDVMRDLCRHGVTMLTLGQYLQPSRHHLPVKRYVSPQEFDEMKQEALAMGFTHAACGPFVRSSYHADLQAKGIEV
K

Sequences:

>Translated_321_residues
MSKPIQMERGVKYRDADKMALIPIKTVAVERQEILRKPSWMKIKLPADSTRIQGIKVAMRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLAQTIADMGLRYVVVTSVDRDDLRDGGAQHFADCISAIRAKN
PNIRIETLVPDFRGRMDRALEIINAAPPDVFNHNLENVPRLYRQVRPGADYHWSLKLLENFKVANPQLPTKSGLMVGLGE
TNAEIVDVMRDLCRHGVTMLTLGQYLQPSRHHLPVKRYVSPQEFDEMKQEALAMGFTHAACGPFVRSSYHADLQAKGIEV
K
>Mature_320_residues
SKPIQMERGVKYRDADKMALIPIKTVAVERQEILRKPSWMKIKLPADSTRIQGIKVAMRKNGLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLAQTIADMGLRYVVVTSVDRDDLRDGGAQHFADCISAIRAKNP
NIRIETLVPDFRGRMDRALEIINAAPPDVFNHNLENVPRLYRQVRPGADYHWSLKLLENFKVANPQLPTKSGLMVGLGET
NAEIVDVMRDLCRHGVTMLTLGQYLQPSRHHLPVKRYVSPQEFDEMKQEALAMGFTHAACGPFVRSSYHADLQAKGIEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=43.598615916955, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI37577164, Length=253, Percent_Identity=44.2687747035573, Blast_Score=214, Evalue=6e-56,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=88.4735202492212, Blast_Score=606, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=42.1602787456446, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.8943894389439, Blast_Score=250, Evalue=2e-67,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=45.993031358885, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SODGM (Q2NUV8)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454472.1
- ProteinModelPortal:   Q2NUV8
- STRING:   Q2NUV8
- GeneID:   3868475
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0792
- NMPDR:   fig|343509.6.peg.1714
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   RRESPET
- PhylomeDB:   Q2NUV8
- ProtClustDB:   PRK05481
- BioCyc:   SGLO343509:SG0792-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35981; Mature: 35850

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIQMERGVKYRDADKMALIPIKTVAVERQEILRKPSWMKIKLPADSTRIQGIKVAMR
CCCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHEEEEHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIADMGLRYVVVTSVDRDDLRDGGAQHFADCISAIRAKNPNIRIETLVPDFRGRMDRAL
HHHHHCCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
EIINAAPPDVFNHNLENVPRLYRQVRPGADYHWSLKLLENFKVANPQLPTKSGLMVGLGE
HHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCEEEECC
TNAEIVDVMRDLCRHGVTMLTLGQYLQPSRHHLPVKRYVSPQEFDEMKQEALAMGFTHAA
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGIEVK
HHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKPIQMERGVKYRDADKMALIPIKTVAVERQEILRKPSWMKIKLPADSTRIQGIKVAMR
CCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHEEEEHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIADMGLRYVVVTSVDRDDLRDGGAQHFADCISAIRAKNPNIRIETLVPDFRGRMDRAL
HHHHHCCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
EIINAAPPDVFNHNLENVPRLYRQVRPGADYHWSLKLLENFKVANPQLPTKSGLMVGLGE
HHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCEEEECC
TNAEIVDVMRDLCRHGVTMLTLGQYLQPSRHHLPVKRYVSPQEFDEMKQEALAMGFTHAA
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGIEVK
HHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA