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The map label for this gene is purK [H]

Identifier: 85058679

GI number: 85058679

Start: 1182518

End: 1183588

Strand: Reverse

Name: purK [H]

Synonym: SG0701

Alternate gene names: 85058679

Gene position: 1183588-1182518 (Counterclockwise)

Preceding gene: 85058680

Following gene: 85058676

Centisome position: 28.38

GC content: 61.53

Gene sequence:

>1071_bases
ATGAAGCCCGTCTGCGTTCTGGGCAACGGCCAATTGGGTCGTATGCTGCGGCAAGCCGGCGAGCCGCTGGGCATCGCGGT
GTTTCCAGTCGGTCTCGATGCCGAACCCGCATCCGTTCCGTTCCAGCAAAGCGTGATCACCGCCGAAATCGAGCGCTGGC
CAGAGACCGCCCTCACCCGCGAACTGGCGCGTCATAATGGCTTTGTCAACCGTGATATTTTTCCCCGGCTGGCCGACCGG
TTAACGCAAAAGCAACTGCTGGATCAGTTGCGCCTGGCGACCGCCCCCTGGGAGCCGTTGACGGGCGCAGCCCAATGGCC
GGAGGTGTTTGTTTCCTTCGGCGATCTAGCGATTGTCAAACGCCGGCTGGGCGGCTATGACGGTCGCGGGCAATGGCGGC
TTCATCCAGGTGACAAGGCCGCCCTGCCCCAGGACTGCTACGGCACCTGCATCGTCGAGCAGGGGATCGCCTTTGACGGA
GAAGTGTCGCTGGTGGGCGCCCGTTCCGCCGACGGCGCTCAGGTTTTTTACCCGTTGACCCACAATTTCCACCAGGATGG
CATTCTGCGCGCCAGCGTCGCGCTTCCCACCCCGGATAGCAGCCTGCAACGGCAGGCGGAAACTATGCTCGGCGCTATCC
TGCACGAACTGAACTATGTCGGCGTGATGGCGATGGAGTGTTTTATCGTCGGCGATCGGCTGCTCATCAATGAACTGGCA
CCACGGGTGCATAACAGCGGCCACTGGACGCAAAACGGCTCCTCCATCAGTCAATTCGAGCTGCATTTGCGCGCCATTCT
GGGGCTGCCGCTGCCGACTCCGGTCGTTAGCGCGCCCGCTGTGATGGTGAATGTGATTGGCAGCGGGATGAACACCGACT
GGCTGGCAGACCCGCTAGTCCATTTGCATTGGTACGAAAAAGAGGTCCGCGCCGGGCGCAAGGTCGGGCACCTAAACCTG
TGCCATCCCGATGACGCGGCGCTCAGCCGCAGTCTGCAGCAGTTGATGACGCTGTTACCGGGGGATTATGAAAACTGCAT
CCGCTGGGCGAGACAAACACTGGTAGACTGA

Upstream 100 bases:

>100_bases
TTTTAGCGCTGCATGATACTGCGCTGGCCGGACGTCTGCGCCAGTGGCGCCAACAGCAAACCGATGAGGTGCTGAGCCAT
CCCGATCCGAGGGAACAGGC

Downstream 100 bases:

>100_bases
GGATGGCTCCGCCGGGCAGGGCTGCCCGGCAGCCTTGCTTAGGCTTTCACCGCCTGTAGCGCTGCGTTAAGAGTGGCGCT
CGGACAGATGGCTTTGCTGG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKPVCVLGNGQLGRMLRQAGEPLGIAVFPVGLDAEPASVPFQQSVITAEIERWPETALTRELARHNGFVNRDIFPRLADR
LTQKQLLDQLRLATAPWEPLTGAAQWPEVFVSFGDLAIVKRRLGGYDGRGQWRLHPGDKAALPQDCYGTCIVEQGIAFDG
EVSLVGARSADGAQVFYPLTHNFHQDGILRASVALPTPDSSLQRQAETMLGAILHELNYVGVMAMECFIVGDRLLINELA
PRVHNSGHWTQNGSSISQFELHLRAILGLPLPTPVVSAPAVMVNVIGSGMNTDWLADPLVHLHWYEKEVRAGRKVGHLNL
CHPDDAALSRSLQQLMTLLPGDYENCIRWARQTLVD

Sequences:

>Translated_356_residues
MKPVCVLGNGQLGRMLRQAGEPLGIAVFPVGLDAEPASVPFQQSVITAEIERWPETALTRELARHNGFVNRDIFPRLADR
LTQKQLLDQLRLATAPWEPLTGAAQWPEVFVSFGDLAIVKRRLGGYDGRGQWRLHPGDKAALPQDCYGTCIVEQGIAFDG
EVSLVGARSADGAQVFYPLTHNFHQDGILRASVALPTPDSSLQRQAETMLGAILHELNYVGVMAMECFIVGDRLLINELA
PRVHNSGHWTQNGSSISQFELHLRAILGLPLPTPVVSAPAVMVNVIGSGMNTDWLADPLVHLHWYEKEVRAGRKVGHLNL
CHPDDAALSRSLQQLMTLLPGDYENCIRWARQTLVD
>Mature_356_residues
MKPVCVLGNGQLGRMLRQAGEPLGIAVFPVGLDAEPASVPFQQSVITAEIERWPETALTRELARHNGFVNRDIFPRLADR
LTQKQLLDQLRLATAPWEPLTGAAQWPEVFVSFGDLAIVKRRLGGYDGRGQWRLHPGDKAALPQDCYGTCIVEQGIAFDG
EVSLVGARSADGAQVFYPLTHNFHQDGILRASVALPTPDSSLQRQAETMLGAILHELNYVGVMAMECFIVGDRLLINELA
PRVHNSGHWTQNGSSISQFELHLRAILGLPLPTPVVSAPAVMVNVIGSGMNTDWLADPLVHLHWYEKEVRAGRKVGHLNL
CHPDDAALSRSLQQLMTLLPGDYENCIRWARQTLVD

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=354, Percent_Identity=71.4689265536723, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1788155, Length=212, Percent_Identity=27.8301886792453, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=28.9617486338798, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39203; Mature: 39203

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVCVLGNGQLGRMLRQAGEPLGIAVFPVGLDAEPASVPFQQSVITAEIERWPETALTR
CCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHH
ELARHNGFVNRDIFPRLADRLTQKQLLDQLRLATAPWEPLTGAAQWPEVFVSFGDLAIVK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RRLGGYDGRGQWRLHPGDKAALPQDCYGTCIVEQGIAFDGEVSLVGARSADGAQVFYPLT
HHHCCCCCCCEEEECCCCCCCCCHHHHHEEEECCCCCCCCCEEEEECCCCCCCEEEEECC
HNFHQDGILRASVALPTPDSSLQRQAETMLGAILHELNYVGVMAMECFIVGDRLLINELA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PRVHNSGHWTQNGSSISQFELHLRAILGLPLPTPVVSAPAVMVNVIGSGMNTDWLADPLV
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCHH
HLHWYEKEVRAGRKVGHLNLCHPDDAALSRSLQQLMTLLPGDYENCIRWARQTLVD
HHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPVCVLGNGQLGRMLRQAGEPLGIAVFPVGLDAEPASVPFQQSVITAEIERWPETALTR
CCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHH
ELARHNGFVNRDIFPRLADRLTQKQLLDQLRLATAPWEPLTGAAQWPEVFVSFGDLAIVK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RRLGGYDGRGQWRLHPGDKAALPQDCYGTCIVEQGIAFDGEVSLVGARSADGAQVFYPLT
HHHCCCCCCCEEEECCCCCCCCCHHHHHEEEECCCCCCCCCEEEEECCCCCCCEEEEECC
HNFHQDGILRASVALPTPDSSLQRQAETMLGAILHELNYVGVMAMECFIVGDRLLINELA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PRVHNSGHWTQNGSSISQFELHLRAILGLPLPTPVVSAPAVMVNVIGSGMNTDWLADPLV
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCHH
HLHWYEKEVRAGRKVGHLNLCHPDDAALSRSLQQLMTLLPGDYENCIRWARQTLVD
HHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2464576; 2644189; 9278503; 1534690; 10569930 [H]