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The map label for this gene is prgK [H]

Identifier: 85058534

GI number: 85058534

Start: 952408

End: 953187

Strand: Reverse

Name: prgK [H]

Synonym: SG0556

Alternate gene names: 85058534

Gene position: 953187-952408 (Counterclockwise)

Preceding gene: 85058535

Following gene: 85058533

Centisome position: 22.85

GC content: 48.97

Gene sequence:

>780_bases
ATGTTTAAGCGACTTCTATTAACCTGTATGGTCATTATCGCCGCTTTGCTGACGACAGGCTGTGACAACACCATTTTGCT
GAACAATTTAAGCCAGGAACAGGCCAACAAAGTTTTAGCGGTATTACAGCAGCATAATATCGCCTCGCAAAAAAGCGGTT
CGCTGAAGGAAGGGTATGCAATTACGGTTCCCAAAAGCGATACCACCACGGCGCTGTCAATTCTGAATCAGTATCAGCTC
CCGGCCAAACCTGAAGTCCAAATTTCTCAGGCGTTCCCCGATAACGCCTTAATTGCTTCGCCGCAGGCTGAACGGGCACG
TATGTTGTCATTACAGGAGCAGCGTTTAGAACAATCGTTAAAGATTATCAGCCAAGTGGTGAATGCTCGTGTTCACGTCA
GTTATCCGCTGAACGATAATACGTTTATTACAGACCCACCCGCTGAGCGGGTCTCTATTTTGATTACCTACAGCGGCAGT
CTCAATGAAAATATGTTCATCAGCAAAATAAAAACGTTGATCAAAAACGGTCTGGCCAACGTTCGCTTTGAAGATATTTC
CGTTGTGCTGTTCCAGGCGCCTCCCTTGCAATATAGCGCGCCAACGCAGGGTGCCCAGCCGTTTATAACACCTTGGTTGT
ATGCCCTTTGCGGAGTGCTATTGGCCGCATTGCTGGCAGGCGGCTCGGTGTTATATCTGCTGGTCAAAAAACAGCGACCG
GAGACAGCGCAGGAGCTCACGCCACAGGATCCACCGGCGCAAGAGGAGAAACCGGTATGA

Upstream 100 bases:

>100_bases
TGCAATCGCTTATCGGTGAATATTCCAACTATGTCTCATTAGTGTCCACTATTGCCAAAAAGGGAGTCAATACCGTCGAG
ACACTGGGAAAAGCACAATA

Downstream 100 bases:

>100_bases
CCCCGCTCCGGTTTGCCAACGTCATGCGCGTGATGTACCACCCTGTCAGTTACCTGCACGCCAGCCGTTTCCCTTCCGGC
TGGCACCGCGAGGATCAAGC

Product: putative type III secretion apparatus

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MFKRLLLTCMVIIAALLTTGCDNTILLNNLSQEQANKVLAVLQQHNIASQKSGSLKEGYAITVPKSDTTTALSILNQYQL
PAKPEVQISQAFPDNALIASPQAERARMLSLQEQRLEQSLKIISQVVNARVHVSYPLNDNTFITDPPAERVSILITYSGS
LNENMFISKIKTLIKNGLANVRFEDISVVLFQAPPLQYSAPTQGAQPFITPWLYALCGVLLAALLAGGSVLYLLVKKQRP
ETAQELTPQDPPAQEEKPV

Sequences:

>Translated_259_residues
MFKRLLLTCMVIIAALLTTGCDNTILLNNLSQEQANKVLAVLQQHNIASQKSGSLKEGYAITVPKSDTTTALSILNQYQL
PAKPEVQISQAFPDNALIASPQAERARMLSLQEQRLEQSLKIISQVVNARVHVSYPLNDNTFITDPPAERVSILITYSGS
LNENMFISKIKTLIKNGLANVRFEDISVVLFQAPPLQYSAPTQGAQPFITPWLYALCGVLLAALLAGGSVLYLLVKKQRP
ETAQELTPQDPPAQEEKPV
>Mature_259_residues
MFKRLLLTCMVIIAALLTTGCDNTILLNNLSQEQANKVLAVLQQHNIASQKSGSLKEGYAITVPKSDTTTALSILNQYQL
PAKPEVQISQAFPDNALIASPQAERARMLSLQEQRLEQSLKIISQVVNARVHVSYPLNDNTFITDPPAERVSILITYSGS
LNENMFISKIKTLIKNGLANVRFEDISVVLFQAPPLQYSAPTQGAQPFITPWLYALCGVLLAALLAGGSVLYLLVKKQRP
ETAQELTPQDPPAQEEKPV

Specific function: Required for invasion of epithelial cells. Could be involved in protein secretion [H]

COG id: COG4669

COG function: function code U; Type III secretory pathway, lipoprotein EscJ

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the yscJ lipoprotein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003282
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF [H]

EC number: NA

Molecular weight: Translated: 28408; Mature: 28408

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRLLLTCMVIIAALLTTGCDNTILLNNLSQEQANKVLAVLQQHNIASQKSGSLKEGYA
CHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
ITVPKSDTTTALSILNQYQLPAKPEVQISQAFPDNALIASPQAERARMLSLQEQRLEQSL
EEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
KIISQVVNARVHVSYPLNDNTFITDPPAERVSILITYSGSLNENMFISKIKTLIKNGLAN
HHHHHHHCCEEEEEECCCCCCEECCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHCCCCC
VRFEDISVVLFQAPPLQYSAPTQGAQPFITPWLYALCGVLLAALLAGGSVLYLLVKKQRP
CEEECEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
ETAQELTPQDPPAQEEKPV
CHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKRLLLTCMVIIAALLTTGCDNTILLNNLSQEQANKVLAVLQQHNIASQKSGSLKEGYA
CHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
ITVPKSDTTTALSILNQYQLPAKPEVQISQAFPDNALIASPQAERARMLSLQEQRLEQSL
EEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
KIISQVVNARVHVSYPLNDNTFITDPPAERVSILITYSGSLNENMFISKIKTLIKNGLAN
HHHHHHHCCEEEEEECCCCCCEECCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHCCCCC
VRFEDISVVLFQAPPLQYSAPTQGAQPFITPWLYALCGVLLAALLAGGSVLYLLVKKQRP
CEEECEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
ETAQELTPQDPPAQEEKPV
CHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476203; 11677609 [H]