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The map label for this gene is iap [H]

Identifier: 85058497

GI number: 85058497

Start: 904257

End: 905009

Strand: Reverse

Name: iap [H]

Synonym: SG0519

Alternate gene names: 85058497

Gene position: 905009-904257 (Counterclockwise)

Preceding gene: 85058509

Following gene: 85058493

Centisome position: 21.7

GC content: 64.41

Gene sequence:

>753_bases
ATGCGTTACCCCGCTTGCCTGCGTATTGCGCTCTGGCTGTTCGTAAGCGCTGGACTGACGCAGGCCGCCCAGGCCGCTTC
TCCTCTCTCTCCCGAACGCTACGCCCAAGAGCAAACCCGCGCTATCGCCACCTATTTTCCCGGGCGCATGGCGGGCAGTC
CTACGGAGCTGCTGGCCGCCGAGCACCTGCAGCAACAATTTCGCGCGCTGGGCTATCAGAGCTATCTGCGCGCTTTCAAT
ACCCGCTATCGCTATGGCGCGCCGGGCGAACGAGTGGACTGGCAGCGCATTACCGCCAGTTCTACTATCGCCGCCAAAGC
CGGCCTGGCCGGGCAAGGGGAGATTCTGGTTATCGCCCACCTCGACACCTACCTGCCGCGCAACGATCGCGATCTGAACC
ACAACCTCGGGGGGTTGACGCTGCAGGGGGTGGACGACAATGCCGCCGGGATCGGTATCATGCTGGCGCTGGCGCGCGAT
CTGGGACGCCTGCCGCTGCGCTCCGGCGTGCGTTTCGTGGCGTTGAGCGCCGTCACGCCCGATTTGGCAGGCAGCCAGGA
CTACCTTGATCGCGTGTCGCCGCCGGAGAAACAGCAAACGCGGCTGGTGGTGGATCTACAGCAGCTCATCGCCTGCGAAA
AGCTGCGCGTCGATTATCAGCTGCTGGCAGGCGCGAACGCGCTATTGCCGCAAATTAACGCCCTGCGCGCGCAGGCCCGC
CGCGCCAGCATACCGCTGCTGATCCATCAGTAA

Upstream 100 bases:

>100_bases
GCGAAACGGAATTGTTTTGCCGCCGTCGCCGCCCCATACTATAGCCGACAGTTGCGCGGCCGAATATTGAGGCCATTCCG
GTAAACAAGGACTTTCCGTT

Downstream 100 bases:

>100_bases
CTTCGCGCGGGCTGGCGTGCCCGTCACCATTGCTGCCGTTCGCCCGCGCCGAGCTACCGGTGCTGGATATCACTTCCGGC
GTTGCCGGCGACAGTTGCCT

Product: putative alkaline phosphatase isozyme conversion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRYPACLRIALWLFVSAGLTQAAQAASPLSPERYAQEQTRAIATYFPGRMAGSPTELLAAEHLQQQFRALGYQSYLRAFN
TRYRYGAPGERVDWQRITASSTIAAKAGLAGQGEILVIAHLDTYLPRNDRDLNHNLGGLTLQGVDDNAAGIGIMLALARD
LGRLPLRSGVRFVALSAVTPDLAGSQDYLDRVSPPEKQQTRLVVDLQQLIACEKLRVDYQLLAGANALLPQINALRAQAR
RASIPLLIHQ

Sequences:

>Translated_250_residues
MRYPACLRIALWLFVSAGLTQAAQAASPLSPERYAQEQTRAIATYFPGRMAGSPTELLAAEHLQQQFRALGYQSYLRAFN
TRYRYGAPGERVDWQRITASSTIAAKAGLAGQGEILVIAHLDTYLPRNDRDLNHNLGGLTLQGVDDNAAGIGIMLALARD
LGRLPLRSGVRFVALSAVTPDLAGSQDYLDRVSPPEKQQTRLVVDLQQLIACEKLRVDYQLLAGANALLPQINALRAQAR
RASIPLLIHQ
>Mature_250_residues
MRYPACLRIALWLFVSAGLTQAAQAASPLSPERYAQEQTRAIATYFPGRMAGSPTELLAAEHLQQQFRALGYQSYLRAFN
TRYRYGAPGERVDWQRITASSTIAAKAGLAGQGEILVIAHLDTYLPRNDRDLNHNLGGLTLQGVDDNAAGIGIMLALARD
LGRLPLRSGVRFVALSAVTPDLAGSQDYLDRVSPPEKQQTRLVVDLQQLIACEKLRVDYQLLAGANALLPQINALRAQAR
RASIPLLIHQ

Specific function: This protein, presumably an aminopeptidase, mediates the conversion of E.coli alkaline phosphatase isozyme 1, to isozymes 2 and 3 by removing, one by one, the two amino-terminal arginine residues [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28C subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789111, Length=192, Percent_Identity=49.4791666666667, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007484 [H]

Pfam domain/function: PF04389 Peptidase_M28 [H]

EC number: 3.4.11.- [C]

Molecular weight: Translated: 27371; Mature: 27371

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYPACLRIALWLFVSAGLTQAAQAASPLSPERYAQEQTRAIATYFPGRMAGSPTELLAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EHLQQQFRALGYQSYLRAFNTRYRYGAPGERVDWQRITASSTIAAKAGLAGQGEILVIAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCEEEEEE
LDTYLPRNDRDLNHNLGGLTLQGVDDNAAGIGIMLALARDLGRLPLRSGVRFVALSAVTP
CHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHCCCEEEEEECCCC
DLAGSQDYLDRVSPPEKQQTRLVVDLQQLIACEKLRVDYQLLAGANALLPQINALRAQAR
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RASIPLLIHQ
HCCCCEEEEC
>Mature Secondary Structure
MRYPACLRIALWLFVSAGLTQAAQAASPLSPERYAQEQTRAIATYFPGRMAGSPTELLAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EHLQQQFRALGYQSYLRAFNTRYRYGAPGERVDWQRITASSTIAAKAGLAGQGEILVIAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCEEEEEE
LDTYLPRNDRDLNHNLGGLTLQGVDDNAAGIGIMLALARDLGRLPLRSGVRFVALSAVTP
CHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHCCCEEEEEECCCC
DLAGSQDYLDRVSPPEKQQTRLVVDLQQLIACEKLRVDYQLLAGANALLPQINALRAQAR
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RASIPLLIHQ
HCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316184; 9278503; 1316900 [H]