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The map label for this gene is shiA [H]

Identifier: 85058476

GI number: 85058476

Start: 862660

End: 863676

Strand: Reverse

Name: shiA [H]

Synonym: SG0498

Alternate gene names: 85058476

Gene position: 863676-862660 (Counterclockwise)

Preceding gene: 85058477

Following gene: 85058475

Centisome position: 20.71

GC content: 51.92

Gene sequence:

>1017_bases
TTGTATGCAGGAGAACGTTCGATGAACCCGATATCGCAACAACCCGTCAGTATCGATAAAAATGTCAAACGCGCAATCTT
TGCGTCGACGCTGGGCACCATCATAGAGTGGTATGACTACGGACTGTATGCCGCGGCCTCCGGGCTGATTATCAATAAAC
TGTTCTTTCCGCAACTGAGCCAACTGGGCGGTATGCTGGAAGCGTTTGCCACCTTCGCCATCGGATTTATTATTCGCCCG
GTGGGGGGGATAGTGATTTCCCATATCGGCGATAAATTTGGCCGCAAACCGGAGCTCATCCTGCCGACATGGCACAGTAT
CGGCATCTGGGCGCCGATACTCCTTATCTTCCTGCGCATTCTGCAGGGTTTCGGCGCCGGCGCCGAACTGGCAGGCGCCA
TTACCCTGGTCGTGGAATACACCCCGGTCAAACGGCGCGGCTTTTTTACCGTTATCCCCAATACAGCCACCAATTTTGGC
GTATTAATCGCGACCGTAACGTTTTTGTTCTTTTCTTGGCTGCCGGAGGAAACGTTATTAGGCTGGGTATGGCGTATCTC
TTTCCAGCTGTCGGTATTGCTGTTCGTTATTGCGCTTTATATCCGCGCCAAGCTGGACGAAACGCCGGAATATGTAAACG
CCATAGATAAAGCGCAGCGTGAGCGCCAGCAGCAAAAAGCGCCGATTCGTCAACTGTTCAAAGAAAGTCCCCGCGAGCTG
CTCTGTGGCTTCTTTGCCATGGGGGGCCATCAGGCGCTGACCTATGTCCTCAATACTTTTGCGCTGAGTTATATGATTAA
TACCCTCGGTATGTCGAAAACCGACGATCTGATGGTGTTGATCATCGCATTTACCTTCACGCTTATCTGCGGGCCGATTG
GCGGTTGGCTGGCCGACCGGTATGGCAGCACCAAGATATTTATCTGTGGCGCGCTGTTTTTTTGTCTACCCCCTGTTTTA
TCTGGTCGATACCAAAAATACTTTATTAGCGGCGCTGGGCGTAAGCGCCATCTATGA

Upstream 100 bases:

>100_bases
CCACCCGTTATAACGGTTTTTCGCCGGCCACCCTGCGGCAGCGATGGCCCCCTGAGGCAAAGCATTCTTCTTATTATGCT
GTGAGGAAGAACATCTCGTC

Downstream 100 bases:

>100_bases
CATCAGTTGGGGCTGCACCGGCGGCGCACAAGGCGCTTTTCTCTCTAATTTATTTCCCACCCGTTATCGGTTCTCCGGCA
TTGCCATGTGTCGAGAATTA

Product: putative ABC transport system permease component

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MYAGERSMNPISQQPVSIDKNVKRAIFASTLGTIIEWYDYGLYAAASGLIINKLFFPQLSQLGGMLEAFATFAIGFIIRP
VGGIVISHIGDKFGRKPELILPTWHSIGIWAPILLIFLRILQGFGAGAELAGAITLVVEYTPVKRRGFFTVIPNTATNFG
VLIATVTFLFFSWLPEETLLGWVWRISFQLSVLLFVIALYIRAKLDETPEYVNAIDKAQRERQQQKAPIRQLFKESPREL
LCGFFAMGGHQALTYVLNTFALSYMINTLGMSKTDDLMVLIIAFTFTLICGPIGGWLADRYGSTKIFICGALFFCLPPVL
SGRYQKYFISGAGRKRHL

Sequences:

>Translated_338_residues
MYAGERSMNPISQQPVSIDKNVKRAIFASTLGTIIEWYDYGLYAAASGLIINKLFFPQLSQLGGMLEAFATFAIGFIIRP
VGGIVISHIGDKFGRKPELILPTWHSIGIWAPILLIFLRILQGFGAGAELAGAITLVVEYTPVKRRGFFTVIPNTATNFG
VLIATVTFLFFSWLPEETLLGWVWRISFQLSVLLFVIALYIRAKLDETPEYVNAIDKAQRERQQQKAPIRQLFKESPREL
LCGFFAMGGHQALTYVLNTFALSYMINTLGMSKTDDLMVLIIAFTFTLICGPIGGWLADRYGSTKIFICGALFFCLPPVL
SGRYQKYFISGAGRKRHL
>Mature_338_residues
MYAGERSMNPISQQPVSIDKNVKRAIFASTLGTIIEWYDYGLYAAASGLIINKLFFPQLSQLGGMLEAFATFAIGFIIRP
VGGIVISHIGDKFGRKPELILPTWHSIGIWAPILLIFLRILQGFGAGAELAGAITLVVEYTPVKRRGFFTVIPNTATNFG
VLIATVTFLFFSWLPEETLLGWVWRISFQLSVLLFVIALYIRAKLDETPEYVNAIDKAQRERQQQKAPIRQLFKESPREL
LCGFFAMGGHQALTYVLNTFALSYMINTLGMSKTDDLMVLIIAFTFTLICGPIGGWLADRYGSTKIFICGALFFCLPPVL
SGRYQKYFISGAGRKRHL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=310, Percent_Identity=32.9032258064516, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1790550, Length=335, Percent_Identity=31.9402985074627, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789941, Length=321, Percent_Identity=31.7757009345794, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788942, Length=323, Percent_Identity=28.1733746130031, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 37748; Mature: 37748

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAGERSMNPISQQPVSIDKNVKRAIFASTLGTIIEWYDYGLYAAASGLIINKLFFPQLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGGMLEAFATFAIGFIIRPVGGIVISHIGDKFGRKPELILPTWHSIGIWAPILLIFLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHH
LQGFGAGAELAGAITLVVEYTPVKRRGFFTVIPNTATNFGVLIATVTFLFFSWLPEETLL
HHCCCCCHHHHHEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
GWVWRISFQLSVLLFVIALYIRAKLDETPEYVNAIDKAQRERQQQKAPIRQLFKESPREL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LCGFFAMGGHQALTYVLNTFALSYMINTLGMSKTDDLMVLIIAFTFTLICGPIGGWLADR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGSTKIFICGALFFCLPPVLSGRYQKYFISGAGRKRHL
CCCCEEHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCC
>Mature Secondary Structure
MYAGERSMNPISQQPVSIDKNVKRAIFASTLGTIIEWYDYGLYAAASGLIINKLFFPQLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGGMLEAFATFAIGFIIRPVGGIVISHIGDKFGRKPELILPTWHSIGIWAPILLIFLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHH
LQGFGAGAELAGAITLVVEYTPVKRRGFFTVIPNTATNFGVLIATVTFLFFSWLPEETLL
HHCCCCCHHHHHEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
GWVWRISFQLSVLLFVIALYIRAKLDETPEYVNAIDKAQRERQQQKAPIRQLFKESPREL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LCGFFAMGGHQALTYVLNTFALSYMINTLGMSKTDDLMVLIIAFTFTLICGPIGGWLADR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGSTKIFICGALFFCLPPVLSGRYQKYFISGAGRKRHL
CCCCEEHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]