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The map label for this gene is lptD

Identifier: 85058405

GI number: 85058405

Start: 749314

End: 751656

Strand: Reverse

Name: lptD

Synonym: SG0427

Alternate gene names: 85058405

Gene position: 751656-749314 (Counterclockwise)

Preceding gene: 85058407

Following gene: 85058404

Centisome position: 18.02

GC content: 55.74

Gene sequence:

>2343_bases
ATGAAAAAACGTTTACCCACACTGCTGGCCAGCCTTATCGGGTCCGCTCTTTATAGCCAGCAGGCGCTGGCCGATCTGGC
GGATCAATGCCTGTTGGGCGTGCCGGCCTATACCAAACCGCTGGTCAGCGGCGATGCCAATTCGCTGCCGGTGCATATCC
AGGCCGACAAGGCCGCAGCGAATTATCCCGAACATGCGCTCTTTAGCGGCAACGTGTATATTGAACAGGGCAACAGCACG
CTCACTGCCGATGAAGTGCAGCTCACGCAGCGCCAGGAGCAGAACAACGCGCCGCTGCGTACCGCCACGGCCACGGGGAA
TGTCAATTATTCTAGCAACGAGATCAAGCTGCAGGGGCCAAAAGCCTGGTCCAATCTCAATACCAAAGATACGGATGTCT
ATCAGGGCAACTACCAGATAGTGGGCAGACAAGGCCGCGGCGACGCCGATACTATGAAACAGCGCGGCGATAACCGCTAT
ACGGTATTGGAAAACGGCAGTTTCACCTCCTGTTTGCCCGGGGACGACAGCTGGAGCGTTGTGGGATCGGAGGTCATCCA
CGACCGCGAAGAGCAGGTCGCCGAGATTTGGAACGCGCGCTTTAAAATCGGCAAAGTGCCGGTATTCTACAGCCCGTATT
TGCAAATACCGGTGGGCGACAAGCGCCGTTCCGGCTTTCTTATCCCCAATGCCAAATACGGCAGCAACAACGGCTTCGAG
TTCAGCGCACCCTATTACCTGAATCTGGCGCCGAATTATGACGCCACGATCATGCCGAATTACATGAGTAAACGCGGTAC
CCAGATCCAGACCGAGTTCCGCTACCACACCACACCGGGCGAAGGGCTGGTGGAGTTCGACTGGCTGCCTAAAGACCGGG
TTTACAGCAGCGAGCACGCCAGCGACGGCGATAGCGATCGCTGGTTGCTCTACTGGCGCCACAACGGGGTCATGGATCAG
GTATGGCGTTTTAACGTCGATTACACCAAAGTCAGCGACTCCAACTACTTTGACGATCTGGATTCCAAATACGGTAGCAC
CACCGACGGCTACGCCACGCAGAAATTCAGTTTCGGCTATGCCGACGAGAACTGGGACACCGCCCTGTCATACAAACAGT
TCCAGGTCTTTGACACCAACAGCAGCGACGCTTACCGCACCGCTCCTCAGCTCGATGTGACCTACTATAAAAACGACGTT
GGTCCGTTTGACTTCAAAGTATTCAGCCAGGCGGCCAAATTCACCAACGTTAACAACAGTTACCCTGAAGCGACTCGGTT
ACACATCGAGCCCACGCTCAACCTGCCGCTGGCCAACCGTTGGGGCAGCTTAAATACTGAAGCCAAGCTGATGGCCACGC
ATTATCAGCAGGAAAATATCGACGAATATAATGAAAATACCGGCACCGGCCGCCATCTGAAAGGCTCGGTAAACCGCGTG
TTGCCGCAGTTCAAAACCGACGGCAAGATGGTGTTCGAGCGCGATATGGATTACGCACCGGACTACACGCAGACGCTGGA
ACCGCGCCTGCAGTACCTGTATGTGCCCTATCGCAACCAGAACGATATCGGCGTCTATGACTCCACCATCCTGCAAACCG
ACTACTCCGGTCTGTTCCGCGATCGAACTTACAGCGGCCTGGACCGCATCTCCTCCGCCAACCAATTGGCGGGCGGTGTC
ACCACCCGAATTTATGACGATCAGCGCGCGGAACGATTTAACGCTTCCGTCGGTCAAATCTACTACTTCTCGCGGCCTCG
TACCGGTGATATCACCGGCACCTGGGATAATTATGATAATACCGGCAGCGTGGTATGGGCGGGAGATAGCTACTGGCGTA
TCAGCAATCAGTGGGGGATCCGCGGCGGCCTGCAGTATGACTCCCGGCTGAACAGCGTTGCGTTGGGCGATGCGGTGCTG
GAGTACCGACGCGACGAAAACCGCATCCTGCAGCTAAATTACCATTACGCCAGCCCGCAATATATTGAGCAAATGCTGTC
CGATATTAGCCATACCGGCTATCAGCAGGGCATTTCGCAGGTGGGTGTAACCGGCAGCTGGCAGCTGGTGGACCGCTGGT
CGCTGGTTGGGGCGTATTATTACGATACCAAGGCGAATCAGCCGGCGGACCAACTGGTCGGCCTGCAATACAACACCTGC
TGCTGGGCTATCAATGTGGGCTATGAGCGGAAGATTACCGGCTGGAACAGCACCGACAGCTCCAGCCAGTACGACAACAA
AGTGTCGTTTAATATTGAACTGCGCGGTTTGAGCAGCAATTATGGCCTGGGTACGGACAAGATGCTGGCCTCCGGCATTC
TGCCCTATCAGCGGGCGTTCTAA

Upstream 100 bases:

>100_bases
AAACGGCAGGATTTAACACTGGCGCAACGCTGGTGAGTGATATAGCCTCTAACCATTTGCCGCGATGAGCCCTGGATGAC
GGAACACCTGAAAAACGCTT

Downstream 100 bases:

>100_bases
GCTGCAGTCTCGACGCAAACATTGAACTTAATCCGCATATGCGGCTGATATATATGGAAAAGTTATGAAAAACTGGAGAA
CGTTTATCCTCGGCCTCGCC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 780; Mature: 780

Protein sequence:

>780_residues
MKKRLPTLLASLIGSALYSQQALADLADQCLLGVPAYTKPLVSGDANSLPVHIQADKAAANYPEHALFSGNVYIEQGNST
LTADEVQLTQRQEQNNAPLRTATATGNVNYSSNEIKLQGPKAWSNLNTKDTDVYQGNYQIVGRQGRGDADTMKQRGDNRY
TVLENGSFTSCLPGDDSWSVVGSEVIHDREEQVAEIWNARFKIGKVPVFYSPYLQIPVGDKRRSGFLIPNAKYGSNNGFE
FSAPYYLNLAPNYDATIMPNYMSKRGTQIQTEFRYHTTPGEGLVEFDWLPKDRVYSSEHASDGDSDRWLLYWRHNGVMDQ
VWRFNVDYTKVSDSNYFDDLDSKYGSTTDGYATQKFSFGYADENWDTALSYKQFQVFDTNSSDAYRTAPQLDVTYYKNDV
GPFDFKVFSQAAKFTNVNNSYPEATRLHIEPTLNLPLANRWGSLNTEAKLMATHYQQENIDEYNENTGTGRHLKGSVNRV
LPQFKTDGKMVFERDMDYAPDYTQTLEPRLQYLYVPYRNQNDIGVYDSTILQTDYSGLFRDRTYSGLDRISSANQLAGGV
TTRIYDDQRAERFNASVGQIYYFSRPRTGDITGTWDNYDNTGSVVWAGDSYWRISNQWGIRGGLQYDSRLNSVALGDAVL
EYRRDENRILQLNYHYASPQYIEQMLSDISHTGYQQGISQVGVTGSWQLVDRWSLVGAYYYDTKANQPADQLVGLQYNTC
CWAINVGYERKITGWNSTDSSSQYDNKVSFNIELRGLSSNYGLGTDKMLASGILPYQRAF

Sequences:

>Translated_780_residues
MKKRLPTLLASLIGSALYSQQALADLADQCLLGVPAYTKPLVSGDANSLPVHIQADKAAANYPEHALFSGNVYIEQGNST
LTADEVQLTQRQEQNNAPLRTATATGNVNYSSNEIKLQGPKAWSNLNTKDTDVYQGNYQIVGRQGRGDADTMKQRGDNRY
TVLENGSFTSCLPGDDSWSVVGSEVIHDREEQVAEIWNARFKIGKVPVFYSPYLQIPVGDKRRSGFLIPNAKYGSNNGFE
FSAPYYLNLAPNYDATIMPNYMSKRGTQIQTEFRYHTTPGEGLVEFDWLPKDRVYSSEHASDGDSDRWLLYWRHNGVMDQ
VWRFNVDYTKVSDSNYFDDLDSKYGSTTDGYATQKFSFGYADENWDTALSYKQFQVFDTNSSDAYRTAPQLDVTYYKNDV
GPFDFKVFSQAAKFTNVNNSYPEATRLHIEPTLNLPLANRWGSLNTEAKLMATHYQQENIDEYNENTGTGRHLKGSVNRV
LPQFKTDGKMVFERDMDYAPDYTQTLEPRLQYLYVPYRNQNDIGVYDSTILQTDYSGLFRDRTYSGLDRISSANQLAGGV
TTRIYDDQRAERFNASVGQIYYFSRPRTGDITGTWDNYDNTGSVVWAGDSYWRISNQWGIRGGLQYDSRLNSVALGDAVL
EYRRDENRILQLNYHYASPQYIEQMLSDISHTGYQQGISQVGVTGSWQLVDRWSLVGAYYYDTKANQPADQLVGLQYNTC
CWAINVGYERKITGWNSTDSSSQYDNKVSFNIELRGLSSNYGLGTDKMLASGILPYQRAF
>Mature_780_residues
MKKRLPTLLASLIGSALYSQQALADLADQCLLGVPAYTKPLVSGDANSLPVHIQADKAAANYPEHALFSGNVYIEQGNST
LTADEVQLTQRQEQNNAPLRTATATGNVNYSSNEIKLQGPKAWSNLNTKDTDVYQGNYQIVGRQGRGDADTMKQRGDNRY
TVLENGSFTSCLPGDDSWSVVGSEVIHDREEQVAEIWNARFKIGKVPVFYSPYLQIPVGDKRRSGFLIPNAKYGSNNGFE
FSAPYYLNLAPNYDATIMPNYMSKRGTQIQTEFRYHTTPGEGLVEFDWLPKDRVYSSEHASDGDSDRWLLYWRHNGVMDQ
VWRFNVDYTKVSDSNYFDDLDSKYGSTTDGYATQKFSFGYADENWDTALSYKQFQVFDTNSSDAYRTAPQLDVTYYKNDV
GPFDFKVFSQAAKFTNVNNSYPEATRLHIEPTLNLPLANRWGSLNTEAKLMATHYQQENIDEYNENTGTGRHLKGSVNRV
LPQFKTDGKMVFERDMDYAPDYTQTLEPRLQYLYVPYRNQNDIGVYDSTILQTDYSGLFRDRTYSGLDRISSANQLAGGV
TTRIYDDQRAERFNASVGQIYYFSRPRTGDITGTWDNYDNTGSVVWAGDSYWRISNQWGIRGGLQYDSRLNSVALGDAVL
EYRRDENRILQLNYHYASPQYIEQMLSDISHTGYQQGISQVGVTGSWQLVDRWSLVGAYYYDTKANQPADQLVGLQYNTC
CWAINVGYERKITGWNSTDSSSQYDNKVSFNIELRGLSSNYGLGTDKMLASGILPYQRAF

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=786, Percent_Identity=65.5216284987277, Blast_Score=1083, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_SODGM (Q2NVX3)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454107.1
- ProteinModelPortal:   Q2NVX3
- SMR:   Q2NVX3
- STRING:   Q2NVX3
- GeneID:   3867538
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0427
- NMPDR:   fig|343509.6.peg.991
- eggNOG:   COG1452
- HOGENOM:   HBG563511
- OMA:   TPYYWNI
- PhylomeDB:   Q2NVX3
- ProtClustDB:   PRK03761
- BioCyc:   SGLO343509:SG0427-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543

Pfam domain/function: PF03968 OstA; PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 88445; Mature: 88445

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLPTLLASLIGSALYSQQALADLADQCLLGVPAYTKPLVSGDANSLPVHIQADKAAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHCCCCCCCEEEEEECCCCC
NYPEHALFSGNVYIEQGNSTLTADEVQLTQRQEQNNAPLRTATATGNVNYSSNEIKLQGP
CCCCCEEEECEEEEECCCCEEEHHHHHHHHHHHHCCCCEEEEEECCCCEECCCEEEEECC
KAWSNLNTKDTDVYQGNYQIVGRQGRGDADTMKQRGDNRYTVLENGSFTSCLPGDDSWSV
CCCCCCCCCCCEEEECCEEEEECCCCCCHHHHHHCCCCEEEEEECCCEEEECCCCCCHHH
VGSEVIHDREEQVAEIWNARFKIGKVPVFYSPYLQIPVGDKRRSGFLIPNAKYGSNNGFE
HHHHHHHCHHHHHHHHHCCCEEECCCEEEECCEEEECCCCCCCCCEEECCCCCCCCCCEE
FSAPYYLNLAPNYDATIMPNYMSKRGTQIQTEFRYHTTPGEGLVEFDWLPKDRVYSSEHA
EECCEEEEECCCCCCEECCHHHHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCC
SDGDSDRWLLYWRHNGVMDQVWRFNVDYTKVSDSNYFDDLDSKYGSTTDGYATQKFSFGY
CCCCCCEEEEEEECCCCHHHHEEECCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEECCC
ADENWDTALSYKQFQVFDTNSSDAYRTAPQLDVTYYKNDVGPFDFKVFSQAAKFTNVNNS
CCCCCCCCCCEEEEEEEECCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCC
YPEATRLHIEPTLNLPLANRWGSLNTEAKLMATHYQQENIDEYNENTGTGRHLKGSVNRV
CCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCHHHHCCCCCCCCEECCCHHHH
LPQFKTDGKMVFERDMDYAPDYTQTLEPRLQYLYVPYRNQNDIGVYDSTILQTDYSGLFR
CCCCCCCCCEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCEEEECCCHHHHH
DRTYSGLDRISSANQLAGGVTTRIYDDQRAERFNASVGQIYYFSRPRTGDITGTWDNYDN
CCCHHHHHHHCCHHHHCCCEEEEEECCHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCC
TGSVVWAGDSYWRISNQWGIRGGLQYDSRLNSVALGDAVLEYRRDENRILQLNYHYASPQ
CCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCHH
YIEQMLSDISHTGYQQGISQVGVTGSWQLVDRWSLVGAYYYDTKANQPADQLVGLQYNTC
HHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEEEEEEEECCCCCCCHHHHCCCEECCE
CWAINVGYERKITGWNSTDSSSQYDNKVSFNIELRGLSSNYGLGTDKMLASGILPYQRAF
EEEEECCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MKKRLPTLLASLIGSALYSQQALADLADQCLLGVPAYTKPLVSGDANSLPVHIQADKAAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHCCCCCCCEEEEEECCCCC
NYPEHALFSGNVYIEQGNSTLTADEVQLTQRQEQNNAPLRTATATGNVNYSSNEIKLQGP
CCCCCEEEECEEEEECCCCEEEHHHHHHHHHHHHCCCCEEEEEECCCCEECCCEEEEECC
KAWSNLNTKDTDVYQGNYQIVGRQGRGDADTMKQRGDNRYTVLENGSFTSCLPGDDSWSV
CCCCCCCCCCCEEEECCEEEEECCCCCCHHHHHHCCCCEEEEEECCCEEEECCCCCCHHH
VGSEVIHDREEQVAEIWNARFKIGKVPVFYSPYLQIPVGDKRRSGFLIPNAKYGSNNGFE
HHHHHHHCHHHHHHHHHCCCEEECCCEEEECCEEEECCCCCCCCCEEECCCCCCCCCCEE
FSAPYYLNLAPNYDATIMPNYMSKRGTQIQTEFRYHTTPGEGLVEFDWLPKDRVYSSEHA
EECCEEEEECCCCCCEECCHHHHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCC
SDGDSDRWLLYWRHNGVMDQVWRFNVDYTKVSDSNYFDDLDSKYGSTTDGYATQKFSFGY
CCCCCCEEEEEEECCCCHHHHEEECCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEECCC
ADENWDTALSYKQFQVFDTNSSDAYRTAPQLDVTYYKNDVGPFDFKVFSQAAKFTNVNNS
CCCCCCCCCCEEEEEEEECCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCC
YPEATRLHIEPTLNLPLANRWGSLNTEAKLMATHYQQENIDEYNENTGTGRHLKGSVNRV
CCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCHHHHCCCCCCCCEECCCHHHH
LPQFKTDGKMVFERDMDYAPDYTQTLEPRLQYLYVPYRNQNDIGVYDSTILQTDYSGLFR
CCCCCCCCCEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCEEEECCCHHHHH
DRTYSGLDRISSANQLAGGVTTRIYDDQRAERFNASVGQIYYFSRPRTGDITGTWDNYDN
CCCHHHHHHHCCHHHHCCCEEEEEECCHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCC
TGSVVWAGDSYWRISNQWGIRGGLQYDSRLNSVALGDAVLEYRRDENRILQLNYHYASPQ
CCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCHH
YIEQMLSDISHTGYQQGISQVGVTGSWQLVDRWSLVGAYYYDTKANQPADQLVGLQYNTC
HHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEEEEEEEECCCCCCCHHHHCCCEECCE
CWAINVGYERKITGWNSTDSSSQYDNKVSFNIELRGLSSNYGLGTDKMLASGILPYQRAF
EEEEECCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA