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The map label for this gene is rob [H]

Identifier: 85058380

GI number: 85058380

Start: 712217

End: 713086

Strand: Reverse

Name: rob [H]

Synonym: SG0402

Alternate gene names: 85058380

Gene position: 713086-712217 (Counterclockwise)

Preceding gene: 85058381

Following gene: 85058378

Centisome position: 17.1

GC content: 58.85

Gene sequence:

>870_bases
ATGGATCAAGCCGGTATCATTCGTGACCTGTTAAGCTGGCTCGATAGCCATCTGGACCAGCCGCTGTCGCTGGATAATGT
CGCCGCCAAGGCCGGCTATTCCAAATGGCACCTGCAGCGTATGTTTAAAGAATTGACTGACCACGCCATCGGCGCTTATA
TTCGTGCGCGCAGGTTGTCCAAGGCCGCGGTGGCCCTGCGGCTGACCAGCCGGCCGATATTGGATATCGCGCTGCAGTAT
CGTTTCGATTCTCAGCAGACGTTCACCCGCGCTTTCAAAAAACAGTTCGCCCAGACGCCGGCGCTGTATCGCCGCAAAGA
AGAGTGGGATTCCGCCGGCATCAGACCGCCGATTCGCCTGGAACACATCGCGCTGCCGCAGGCGGAGTTCATCCGGCTGC
CGGCGATAGAACTGGTCGGCGTTACGCAAAGCTATACCTGCACCCTTGAGCAGCTCAGCAGTTTCCGTACCGAGTTGCGC
ATCCATTTCTGGCGCCAGTATCTCGGCGAGGCCAAATGCCTGCCTCCCACCCTGTACGGCCTGAACCATACCCGGCCCAG
TCTGGAAAAGGATGACGAGCAGGAAGTGCTCTACACCACCGCCATCGAACCGCAGCATATGCCGGAAAATGTGCACGGCG
GCGAGCACATCCATTTGGAAGGCGGCGAATACGCCCGCTTTATTTACCAGGGCCCGCCCCACGACCTGCAGGACTTTATC
CTGGCGCTGTACGGCACCTGCCTGCCGTCGCAAAGCCTGGTGCGCCGTAAGGGATGCGATATCGAACGCTTCTTTCCGCT
GGAACGCAAACCGGACGCGGAACTGCCGCACACCATTCGCTGCGAGTATTTGATTCCGGTAAGGCGTTAA

Upstream 100 bases:

>100_bases
ATAATAAGCCATTAAATCTTTTACGATCTGTTAGCCAGATTATTGCTATCATGCGTCGACACTTTTCTAATGACGCCATC
ACCGCTTTCTGAGGATTCTT

Downstream 100 bases:

>100_bases
CGCTGTAGCTCATCCAGGGCGGGCATATCCAGATGGGAGACATCGCCCGCCGTTTCCACCACCCAACCGGGCGCCAGCCA
CGGGCTTTGCTGGTAATCCA

Product: right origin-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDQAGIIRDLLSWLDSHLDQPLSLDNVAAKAGYSKWHLQRMFKELTDHAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRKEEWDSAGIRPPIRLEHIALPQAEFIRLPAIELVGVTQSYTCTLEQLSSFRTELR
IHFWRQYLGEAKCLPPTLYGLNHTRPSLEKDDEQEVLYTTAIEPQHMPENVHGGEHIHLEGGEYARFIYQGPPHDLQDFI
LALYGTCLPSQSLVRRKGCDIERFFPLERKPDAELPHTIRCEYLIPVRR

Sequences:

>Translated_289_residues
MDQAGIIRDLLSWLDSHLDQPLSLDNVAAKAGYSKWHLQRMFKELTDHAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRKEEWDSAGIRPPIRLEHIALPQAEFIRLPAIELVGVTQSYTCTLEQLSSFRTELR
IHFWRQYLGEAKCLPPTLYGLNHTRPSLEKDDEQEVLYTTAIEPQHMPENVHGGEHIHLEGGEYARFIYQGPPHDLQDFI
LALYGTCLPSQSLVRRKGCDIERFFPLERKPDAELPHTIRCEYLIPVRR
>Mature_289_residues
MDQAGIIRDLLSWLDSHLDQPLSLDNVAAKAGYSKWHLQRMFKELTDHAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRKEEWDSAGIRPPIRLEHIALPQAEFIRLPAIELVGVTQSYTCTLEQLSSFRTELR
IHFWRQYLGEAKCLPPTLYGLNHTRPSLEKDDEQEVLYTTAIEPQHMPENVHGGEHIHLEGGEYARFIYQGPPHDLQDFI
LALYGTCLPSQSLVRRKGCDIERFFPLERKPDAELPHTIRCEYLIPVRR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=289, Percent_Identity=66.0899653979239, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1790497, Length=106, Percent_Identity=55.6603773584906, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=48.5148514851485, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33503; Mature: 33503

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQAGIIRDLLSWLDSHLDQPLSLDNVAAKAGYSKWHLQRMFKELTDHAIGAYIRARRLS
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAVALRLTSRPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRKEEWDSAGIRPPIRL
HHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEE
EHIALPQAEFIRLPAIELVGVTQSYTCTLEQLSSFRTELRIHFWRQYLGEAKCLPPTLYG
HEECCCHHHHHCCCCCCEEECCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC
LNHTRPSLEKDDEQEVLYTTAIEPQHMPENVHGGEHIHLEGGEYARFIYQGPPHDLQDFI
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHH
LALYGTCLPSQSLVRRKGCDIERFFPLERKPDAELPHTIRCEYLIPVRR
HHHHHHCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MDQAGIIRDLLSWLDSHLDQPLSLDNVAAKAGYSKWHLQRMFKELTDHAIGAYIRARRLS
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAVALRLTSRPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRKEEWDSAGIRPPIRL
HHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEE
EHIALPQAEFIRLPAIELVGVTQSYTCTLEQLSSFRTELRIHFWRQYLGEAKCLPPTLYG
HEECCCHHHHHCCCCCCEEECCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC
LNHTRPSLEKDDEQEVLYTTAIEPQHMPENVHGGEHIHLEGGEYARFIYQGPPHDLQDFI
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHH
LALYGTCLPSQSLVRRKGCDIERFFPLERKPDAELPHTIRCEYLIPVRR
HHHHHHCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]