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The map label for this gene is rsmD

Identifier: 85058061

GI number: 85058061

Start: 129640

End: 130245

Strand: Reverse

Name: rsmD

Synonym: SG0083

Alternate gene names: 85058061

Gene position: 130245-129640 (Counterclockwise)

Preceding gene: 85058066

Following gene: 85058060

Centisome position: 3.12

GC content: 61.88

Gene sequence:

>606_bases
ATGGCAAAAAACAATACTGCCCGTTCCGCCGGCCAAATCCGTATCATCGGCGGCCGCTGGCGCGGCCGTAAGCTGCCGGT
GCCAGACAGTCCGGGCTTGCGGCCGACGACCGACCGTGTGCGGGAAACGCTGTTTAATTGGCTGGCGCCGGTCATTCAGG
GCGCGCGCTGCCTGGATTGTTTCGCCGGCAGCGGCGCTTTAGGTCTTGAGGCACTCTCGCGCGCCGCCGCCAGCGTCACG
CTATTGGAGCAGGATCGCGCGGTCAGCGCGCAGCTTGCCCGCAATTTGCTGGTGTTGCAGGCACAGCAGGCGGAGGTCAT
CACCACCGACAGCCTGCGTTGGCTTGCGCAGCCGGGCGCCGTTTTCGATGTCGTCTTCATCGATCCCCCCTTTCGTCACG
GGATGATAACCGCTACGGTGACCGCGCTGGAGCAGCACCGGCGTTTGGCGGACGACTCCTGGATTTACATTGAAACCGAA
ACGGAAGGCCCCGCTCCCGAGGTGCCGGCCCATTGGCAGCTACATCGGGAAAAAATTGCCGGTCAAATCTCCTACCGGCT
CTATATCCGTCACGCTACGCCGGAGAAACAACATGATACTGATTAA

Upstream 100 bases:

>100_bases
ATGCGAAATACCGTTTCCCACGTCCCTCCTCCCCGCTAGAATAGACTATCGTTGACATTCGTTGTGGTCTTTGCGCGTTT
TGACTCGTAACAAGATATCT

Downstream 100 bases:

>100_bases
TCTGGGCCGCCTGTTGATGCTGGTGGTGTGGAGCTTTTTGTTATTTAACCTGATACAGCCGTTTCCGAAGCCGCTGAAAT
ATTTTCTCGATATTGGCCTG

Product: 16S rRNA m(2)G966-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MAKNNTARSAGQIRIIGGRWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIQGARCLDCFAGSGALGLEALSRAAASVT
LLEQDRAVSAQLARNLLVLQAQQAEVITTDSLRWLAQPGAVFDVVFIDPPFRHGMITATVTALEQHRRLADDSWIYIETE
TEGPAPEVPAHWQLHREKIAGQISYRLYIRHATPEKQHDTD

Sequences:

>Translated_201_residues
MAKNNTARSAGQIRIIGGRWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIQGARCLDCFAGSGALGLEALSRAAASVT
LLEQDRAVSAQLARNLLVLQAQQAEVITTDSLRWLAQPGAVFDVVFIDPPFRHGMITATVTALEQHRRLADDSWIYIETE
TEGPAPEVPAHWQLHREKIAGQISYRLYIRHATPEKQHDTD
>Mature_200_residues
AKNNTARSAGQIRIIGGRWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIQGARCLDCFAGSGALGLEALSRAAASVTL
LEQDRAVSAQLARNLLVLQAQQAEVITTDSLRWLAQPGAVFDVVFIDPPFRHGMITATVTALEQHRRLADDSWIYIETET
EGPAPEVPAHWQLHREKIAGQISYRLYIRHATPEKQHDTD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=199, Percent_Identity=63.3165829145729, Blast_Score=260, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22321; Mature: 22189

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNNTARSAGQIRIIGGRWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIQGARCLDC
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
FAGSGALGLEALSRAAASVTLLEQDRAVSAQLARNLLVLQAQQAEVITTDSLRWLAQPGA
HCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHEEEECCCEEEECCCHHHHCCCCC
VFDVVFIDPPFRHGMITATVTALEQHRRLADDSWIYIETETEGPAPEVPAHWQLHREKIA
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHC
GQISYRLYIRHATPEKQHDTD
CEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AKNNTARSAGQIRIIGGRWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIQGARCLDC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
FAGSGALGLEALSRAAASVTLLEQDRAVSAQLARNLLVLQAQQAEVITTDSLRWLAQPGA
HCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHEEEECCCEEEECCCHHHHCCCCC
VFDVVFIDPPFRHGMITATVTALEQHRRLADDSWIYIETETEGPAPEVPAHWQLHREKIA
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHC
GQISYRLYIRHATPEKQHDTD
CEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]