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The map label for this gene is fliJ

Identifier: 85058027

GI number: 85058027

Start: 66023

End: 66472

Strand: Reverse

Name: fliJ

Synonym: SG0049

Alternate gene names: 85058027

Gene position: 66472-66023 (Counterclockwise)

Preceding gene: 85058028

Following gene: 85058026

Centisome position: 1.59

GC content: 52.22

Gene sequence:

>450_bases
ATGACCAGTACATCGCCTATCGAAACCTTGAGGGAGCATGCTGAACGGGCTTTGGAAGAAGCCCTGCTTCAGCTCGGCGC
CTGCCGCAGCGCGCACGCCAGCGCCGTGGCGCAGCTAAATCAGTTGATGAGTTACGAGCAAGAATATCGCAAACAACTTG
AGGATAATATGACCGGCACCGGTCTGAATGTTGATAAATGGATTAATTATCAGTCCTTTATTGCCTCTCTGTATAAAATT
GTCGCCCATCACAGCCAGCAGGTGTCGGTGTTTCAATCGCGGGTCGATCTGGCGGTGGCCCAGTGGCACCAAAAAAAACA
GCGGCTTAATGCGTTCGAGATCCTCAAAACGCGTTCCGAGACCGCGCGTCAACTACGCGAAAATCGCCGCAGTCAAAAAC
AAATGGATGAGTTCGCGCTGCGGGCGTCCTTAAGGAGAGTGGAATCATGA

Upstream 100 bases:

>100_bases
TATTTGCGCCAGGATGTCTCGCAACCTTGTAGTTACACCCAATCCTGTCAACAGCTGTCCGATCTCCTCATCCCCCCTTC
AACCGCATAAGGAATCCTTC

Downstream 100 bases:

>100_bases
TACCCACCGCGAGCATGATTATCAGGGACAGAGGGCCCATTATCACGACCGCCGCCGCAACCGAATTGCACCAGAGTGAA
TTTGCTGACGCCTTACAACA

Product: flagellar biosynthesis chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MTSTSPIETLREHAERALEEALLQLGACRSAHASAVAQLNQLMSYEQEYRKQLEDNMTGTGLNVDKWINYQSFIASLYKI
VAHHSQQVSVFQSRVDLAVAQWHQKKQRLNAFEILKTRSETARQLRENRRSQKQMDEFALRASLRRVES

Sequences:

>Translated_149_residues
MTSTSPIETLREHAERALEEALLQLGACRSAHASAVAQLNQLMSYEQEYRKQLEDNMTGTGLNVDKWINYQSFIASLYKI
VAHHSQQVSVFQSRVDLAVAQWHQKKQRLNAFEILKTRSETARQLRENRRSQKQMDEFALRASLRRVES
>Mature_148_residues
TSTSPIETLREHAERALEEALLQLGACRSAHASAVAQLNQLMSYEQEYRKQLEDNMTGTGLNVDKWINYQSFIASLYKIV
AHHSQQVSVFQSRVDLAVAQWHQKKQRLNAFEILKTRSETARQLRENRRSQKQMDEFALRASLRRVES

Specific function: The fliJ protein is a flagellar protein that affects chemotactic events. Mutations in fliJ results in failure to respond to chemotactic stimuli [H]

COG id: COG2882

COG function: function code NUO; Flagellar biosynthesis chaperone

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliJ family [H]

Homologues:

Organism=Escherichia coli, GI1788252, Length=148, Percent_Identity=41.8918918918919, Blast_Score=118, Evalue=2e-28,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018006
- InterPro:   IPR012823 [H]

Pfam domain/function: PF02050 FliJ [H]

EC number: NA

Molecular weight: Translated: 17277; Mature: 17146

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTSPIETLREHAERALEEALLQLGACRSAHASAVAQLNQLMSYEQEYRKQLEDNMTGT
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLNVDKWINYQSFIASLYKIVAHHSQQVSVFQSRVDLAVAQWHQKKQRLNAFEILKTRSE
CCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TARQLRENRRSQKQMDEFALRASLRRVES
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSTSPIETLREHAERALEEALLQLGACRSAHASAVAQLNQLMSYEQEYRKQLEDNMTGT
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLNVDKWINYQSFIASLYKIVAHHSQQVSVFQSRVDLAVAQWHQKKQRLNAFEILKTRSE
CCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TARQLRENRRSQKQMDEFALRASLRRVES
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8631687 [H]