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The map label for this gene is rpoH [H]

Identifier: 84625707

GI number: 84625707

Start: 4568194

End: 4569069

Strand: Reverse

Name: rpoH [H]

Synonym: XOO_4050

Alternate gene names: 84625707

Gene position: 4569069-4568194 (Counterclockwise)

Preceding gene: 84625708

Following gene: 84625704

Centisome position: 92.49

GC content: 62.1

Gene sequence:

>876_bases
ATGAACCAGACTACTTCGACTGCCCTTGTGGCAAACAATCTCCCGATTCCCAGTGCGCTCGGTTCGCTGGACGCCTACAT
CGGTGCCGTGCACCAGATTCCGGTGCTGTCGGTCGATGAGGAGCAGAATCTGGCCCGCCGCTTCCGCGACGAGCTGGATT
TGGACGCCGCGCGCGAACTGGTCCATTCCCACCTGCGCTTCGTGGTGCATGTGGCCCGCGGCTATAACGGCTACGGCCTG
CCGCTGGGCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTCAAGCGCTTCGACCCGGACATGGGCGTGCG
TTTGGTCAGCTTCGCGGTGCACTGGATCCGTGCCGAAATGCACGAGTTCATCCTGAAGAACTGGCGCATCGTCAAGGTCG
CCACGACCAAGGCGCAGCGCAAGCTGTTCTTCAACCTGCGCAAGTCCAAGACCCGTCTGGGCTGGCTCAATGCGTCGGAA
GTGACTGCGGTCGCCAAGGACTTGAACGTCTCAGAGCGTGAAGTGATGGAGATGGAGTCGCGTCTGTCGGGTCGCGACAT
CGGTTTCGACGCCTCGTCCGACGAAGACGACGATCACGGCCCGCCGTCGCCGGTGAGCTACCTGGTCGCCAACGAAGAAG
ACCCGTCGCAGGCGTACGAGCGCCACGACAGCGAAGACAACCAGTTGCAGTTGCTGCGCGAAGGCATGGCCGGTCTGGAT
ACGCGTTCGCGCGACATCGTCAAGCGTCGCTGGCTGGATTCGGATTCCAAGGTGACGCTGCAGGAGCTGGCCGATGAGTA
CGGCGTGTCGGCCGAGCGTATCCGCCAGATCGAGGCGAATGCGCTGAAGAAGATGAAGGCGCTGTTCGTCGCCTGA

Upstream 100 bases:

>100_bases
AACAACGGTGACGCAAAAAGCCCGAACGTTCTATAAATGGAATGCTGGGAACTCTTTCTGTACCCGGCAGTCCAATAGTT
CGACTGCTAAGATGGTGCCT

Downstream 100 bases:

>100_bases
TCCTTTCGATCGTGCGATCGCTGTTGTACAAACGGCCCGGTTTTCCGGGCCGTTTGCAGATGTGGTGTTACGCGATTGCG
ACTCCGGCGCGCGCGCCACC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQNLARRFRDELDLDAARELVHSHLRFVVHVARGYNGYGL
PLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASE
VTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
TRSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA

Sequences:

>Translated_291_residues
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQNLARRFRDELDLDAARELVHSHLRFVVHVARGYNGYGL
PLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASE
VTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
TRSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
>Mature_291_residues
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQNLARRFRDELDLDAARELVHSHLRFVVHVARGYNGYGL
PLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASE
VTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
TRSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1789098, Length=283, Percent_Identity=27.208480565371, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=28.936170212766, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32812; Mature: 32812

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQNLARRFRDELDLDAAREL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCHHHHHHH
VHSHLRFVVHVARGYNGYGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEM
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASEVTAVAKDLNVSEREVMEMES
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
RLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHCCC
TRSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
CCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQNLARRFRDELDLDAAREL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCHHHHHHH
VHSHLRFVVHVARGYNGYGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEM
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASEVTAVAKDLNVSEREVMEMES
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
RLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHCCC
TRSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
CCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168128; 10952301 [H]