Click here to switch to the map view.

The map label for this gene is ligB [H]

Identifier: 84625182

GI number: 84625182

Start: 3986586

End: 3987482

Strand: Reverse

Name: ligB [H]

Synonym: XOO_3525

Alternate gene names: 84625182

Gene position: 3987482-3986586 (Counterclockwise)

Preceding gene: 84625183

Following gene: 84625181

Centisome position: 80.71

GC content: 64.33

Gene sequence:

>897_bases
ATGGCAAGCATCATCGGCGGTATCGGGACCTCGCACGTTCCCACCATCGGGGTGGCCTACGACAAGGGCAAACAGCAGGA
TCCGGTGTGGAAGCCGCTGTTTGACGGCTACGTGCCGGTGGCCGCGTGGCTGGCCGAACAACGGGCCGACGTGCTGGTGT
TCTTCTACAACGACCATTGCACCACGTTCTTTTTCGATCTGTACCCCACCTTCGCGCTCGGCGTGGGCGAACGCTTTCCG
GTTGCCGACGAGGGTGCGGGGCTGCGCAACTTGCCGCCGATTCGTGGCGATGTGCAGTTGCAGGCACATATCGCCGAATG
CCTGGTCAACGACGAATTCGACCTGACCGTGTTCCAGGACAAACCCATCGACCATGGCTGCGCAGCGCCGCTGCCGCTGC
TGTGGCCGCATGTGCCGGACTGGCCGGGCACGGTGGTACCGATCGCCATCAACGTGCTGCAGTACCCGCTGCCGACCGCA
CGGCGCTGCTATCGCCTGGGCCAGGCGGTGCGCCGAGCGATCGAGTCGTATCCGGAAGATCTTCGGGTGGTGGTCGTGGG
CACCGGTGGGCTATCGCACCAGATCCATGGCGAGCGCTCCGGTTTCAACAACACCGATTGGGACATGGAATTTCTGGACC
GGTTCGAGCATGCGCACGAGACCCTCACCGATCTCACCCACACCGATTACGTGCGCCTGGGCGGTGCCGAGAGCGTGGAG
CAGATCATGTGGCTGGCGATGCGCGGCGCGCTCGACGGGCCGATCCGCAAGCTGCATCAAAACTATTACCTGATGACCAC
CACTGCGATGACCGTGGTGCTGTACGAGCCGGGCGAAGAAGTGGCGGTCGCGCCGCGTTCGGCCGAGTGGCTCGCGCGCA
CTGCCGACGCGGCATGA

Upstream 100 bases:

>100_bases
CGGTCTTCCTTTCTTGGATCCATTGTGGTGCAGGTCCCGACTCACGTACTGATGCGCGCCCGCCCCGATCGGCGGCCGGC
CCGCGTCGCAGGAGCGCAGC

Downstream 100 bases:

>100_bases
GGAGGTACTGATGAATCCGCAAACGCATGGCATGGAACAGATCGAAGGCACGTACCTGTTCGATCTGCGCACCAGCAATC
GCGCGCTGCGCCTCAACCGA

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MASIIGGIGTSHVPTIGVAYDKGKQQDPVWKPLFDGYVPVAAWLAEQRADVLVFFYNDHCTTFFFDLYPTFALGVGERFP
VADEGAGLRNLPPIRGDVQLQAHIAECLVNDEFDLTVFQDKPIDHGCAAPLPLLWPHVPDWPGTVVPIAINVLQYPLPTA
RRCYRLGQAVRRAIESYPEDLRVVVVGTGGLSHQIHGERSGFNNTDWDMEFLDRFEHAHETLTDLTHTDYVRLGGAESVE
QIMWLAMRGALDGPIRKLHQNYYLMTTTAMTVVLYEPGEEVAVAPRSAEWLARTADAA

Sequences:

>Translated_298_residues
MASIIGGIGTSHVPTIGVAYDKGKQQDPVWKPLFDGYVPVAAWLAEQRADVLVFFYNDHCTTFFFDLYPTFALGVGERFP
VADEGAGLRNLPPIRGDVQLQAHIAECLVNDEFDLTVFQDKPIDHGCAAPLPLLWPHVPDWPGTVVPIAINVLQYPLPTA
RRCYRLGQAVRRAIESYPEDLRVVVVGTGGLSHQIHGERSGFNNTDWDMEFLDRFEHAHETLTDLTHTDYVRLGGAESVE
QIMWLAMRGALDGPIRKLHQNYYLMTTTAMTVVLYEPGEEVAVAPRSAEWLARTADAA
>Mature_297_residues
ASIIGGIGTSHVPTIGVAYDKGKQQDPVWKPLFDGYVPVAAWLAEQRADVLVFFYNDHCTTFFFDLYPTFALGVGERFPV
ADEGAGLRNLPPIRGDVQLQAHIAECLVNDEFDLTVFQDKPIDHGCAAPLPLLWPHVPDWPGTVVPIAINVLQYPLPTAR
RCYRLGQAVRRAIESYPEDLRVVVVGTGGLSHQIHGERSGFNNTDWDMEFLDRFEHAHETLTDLTHTDYVRLGGAESVEQ
IMWLAMRGALDGPIRKLHQNYYLMTTTAMTVVLYEPGEEVAVAPRSAEWLARTADAA

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=184, Percent_Identity=33.1521739130435, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 33171; Mature: 33040

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIIGGIGTSHVPTIGVAYDKGKQQDPVWKPLFDGYVPVAAWLAEQRADVLVFFYNDHC
CCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHCCCCEEEEEECCCC
TTFFFDLYPTFALGVGERFPVADEGAGLRNLPPIRGDVQLQAHIAECLVNDEFDLTVFQD
EEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
KPIDHGCAAPLPLLWPHVPDWPGTVVPIAINVLQYPLPTARRCYRLGQAVRRAIESYPED
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
LRVVVVGTGGLSHQIHGERSGFNNTDWDMEFLDRFEHAHETLTDLTHTDYVRLGGAESVE
EEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
QIMWLAMRGALDGPIRKLHQNYYLMTTTAMTVVLYEPGEEVAVAPRSAEWLARTADAA
HHHHHHHHCCCCHHHHHHHCCEEEEEEEEEEEEEECCCCCEEECCCCHHHHHHHCCCC
>Mature Secondary Structure 
ASIIGGIGTSHVPTIGVAYDKGKQQDPVWKPLFDGYVPVAAWLAEQRADVLVFFYNDHC
CCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHCCCCEEEEEECCCC
TTFFFDLYPTFALGVGERFPVADEGAGLRNLPPIRGDVQLQAHIAECLVNDEFDLTVFQD
EEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
KPIDHGCAAPLPLLWPHVPDWPGTVVPIAINVLQYPLPTARRCYRLGQAVRRAIESYPED
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
LRVVVVGTGGLSHQIHGERSGFNNTDWDMEFLDRFEHAHETLTDLTHTDYVRLGGAESVE
EEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
QIMWLAMRGALDGPIRKLHQNYYLMTTTAMTVVLYEPGEEVAVAPRSAEWLARTADAA
HHHHHHHHCCCCHHHHHHHCCEEEEEEEEEEEEEECCCCCEEECCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]