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The map label for this gene is pcaQ [H]

Identifier: 84625180

GI number: 84625180

Start: 3985148

End: 3986149

Strand: Reverse

Name: pcaQ [H]

Synonym: XOO_3523

Alternate gene names: 84625180

Gene position: 3986149-3985148 (Counterclockwise)

Preceding gene: 84625181

Following gene: 84625172

Centisome position: 80.69

GC content: 63.37

Gene sequence:

>1002_bases
ATGACCATGTCCTCCGTGTTCTTTGAACATCGCATCAAGGTGCGGCACCTGCGTGTGATCGAAGCGCTGGACAGGCAGAA
AAGCCTGTTGCGCGCCTCACGCGTCTTGAATGTCACACAGCCTGCGCTCACGCGTGCCCTGCAGGAGATCGAAGAGATCG
TTGGCGCGCCATTGTTCGAGCGGCACTCGCGTGGCGTGCGCCCCAACGCGATGGGCGAGGTCCTGGTGCAGACCGCGCAT
GTGGTGCTGGGCCAGCTACGACGCGCGCAGGACAACTTCGAAGGACTGCTGCAGGACGATGCGCTTACCATTACCGTCGG
CGCCTTGCCGGTAGCGGCCAGCGGTTTGCTGCCGGCGGTGCTGGCCGCGTTGTATCGCACTGAACCCACGCTGCGGGTGC
GTTTGCTGCACGGCCGCACCGATGAGCTGTTGCCGAAGCTGGCCGGCAACGAGATCGATCTGGTGGTCGGGCGGTTGTAT
CCGCTGGCCCGTTACGACGCACTGATTCGCAAGGTGTTGTACCAGGATCCGATTGCGCTGGTGGCGCGTAGCGACCACCC
GCTGTTCGCCAATGGCCCAGTGCGCATCGCCGAAGCGGCGGCTTACCGGCAGGTGCTGCCGACCTTGAGCCAACATGTCG
AGCGCGACGTGGCACAGGTGATGCGCGAACACGGCCTGGCAACGCGCGACCAATTGCGTTCCAGTTCGGCCAGTTTCACG
CGCGAAATTCTGCTCGGTACCGACAGCATCGCGGTAATGCCCAGCATGATGGTGGCTGGCGATATCGCACGCGGCGAGTT
GCGTGCCTTCCAGCTGCAACAGGCCTCACAGGCGCGTGCGGGCGGGGTGATCTATCGCGACGGCGCCGCAATCCACAAGC
CTGGTGTGCGCTTGCTGATGCGCGAACTTGAGAATCAGCTCGCGCTCATGGCCGAACAGGGCGCGGTGTGTGTAGATGAT
CTTATCGGCGAAGACCACGTGTTGCTGGACGAGTCTGCGTGA

Upstream 100 bases:

>100_bases
TCATGCAGACACGGCGTGTGCCCGATGCGCGCTGAGCGACGCGTAGCGACGATGCGCCGTATTGCCGCGTTACCCGACTT
CCCAACGTAAGGCCCACCCC

Downstream 100 bases:

>100_bases
TTGCAATACGGCTTGCGCACCGAGCGCCGGATCGCCGCTCATGCCGACGCGCACAAACGCTCGACAGGTGTCGAACGCGT
AGCGGTTCGATGTAGCAGAA

Product: transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTMSSVFFEHRIKVRHLRVIEALDRQKSLLRASRVLNVTQPALTRALQEIEEIVGAPLFERHSRGVRPNAMGEVLVQTAH
VVLGQLRRAQDNFEGLLQDDALTITVGALPVAASGLLPAVLAALYRTEPTLRVRLLHGRTDELLPKLAGNEIDLVVGRLY
PLARYDALIRKVLYQDPIALVARSDHPLFANGPVRIAEAAAYRQVLPTLSQHVERDVAQVMREHGLATRDQLRSSSASFT
REILLGTDSIAVMPSMMVAGDIARGELRAFQLQQASQARAGGVIYRDGAAIHKPGVRLLMRELENQLALMAEQGAVCVDD
LIGEDHVLLDESA

Sequences:

>Translated_333_residues
MTMSSVFFEHRIKVRHLRVIEALDRQKSLLRASRVLNVTQPALTRALQEIEEIVGAPLFERHSRGVRPNAMGEVLVQTAH
VVLGQLRRAQDNFEGLLQDDALTITVGALPVAASGLLPAVLAALYRTEPTLRVRLLHGRTDELLPKLAGNEIDLVVGRLY
PLARYDALIRKVLYQDPIALVARSDHPLFANGPVRIAEAAAYRQVLPTLSQHVERDVAQVMREHGLATRDQLRSSSASFT
REILLGTDSIAVMPSMMVAGDIARGELRAFQLQQASQARAGGVIYRDGAAIHKPGVRLLMRELENQLALMAEQGAVCVDD
LIGEDHVLLDESA
>Mature_332_residues
TMSSVFFEHRIKVRHLRVIEALDRQKSLLRASRVLNVTQPALTRALQEIEEIVGAPLFERHSRGVRPNAMGEVLVQTAHV
VLGQLRRAQDNFEGLLQDDALTITVGALPVAASGLLPAVLAALYRTEPTLRVRLLHGRTDELLPKLAGNEIDLVVGRLYP
LARYDALIRKVLYQDPIALVARSDHPLFANGPVRIAEAAAYRQVLPTLSQHVERDVAQVMREHGLATRDQLRSSSASFTR
EILLGTDSIAVMPSMMVAGDIARGELRAFQLQQASQARAGGVIYRDGAAIHKPGVRLLMRELENQLALMAEQGAVCVDDL
IGEDHVLLDESA

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=307, Percent_Identity=27.3615635179153, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787601, Length=247, Percent_Identity=25.9109311740891, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788887, Length=178, Percent_Identity=30.8988764044944, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693105, Length=183, Percent_Identity=26.2295081967213, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789506, Length=247, Percent_Identity=18.6234817813765, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081766, Length=184, Percent_Identity=27.1739130434783, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=24.390243902439, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36634; Mature: 36503

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSSVFFEHRIKVRHLRVIEALDRQKSLLRASRVLNVTQPALTRALQEIEEIVGAPLFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHH
RHSRGVRPNAMGEVLVQTAHVVLGQLRRAQDNFEGLLQDDALTITVGALPVAASGLLPAV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
LAALYRTEPTLRVRLLHGRTDELLPKLAGNEIDLVVGRLYPLARYDALIRKVLYQDPIAL
HHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHE
VARSDHPLFANGPVRIAEAAAYRQVLPTLSQHVERDVAQVMREHGLATRDQLRSSSASFT
EECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHH
REILLGTDSIAVMPSMMVAGDIARGELRAFQLQQASQARAGGVIYRDGAAIHKPGVRLLM
HHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
RELENQLALMAEQGAVCVDDLIGEDHVLLDESA
HHHHHHHHHHHHCCCEEHHHHCCCCCEEECCCC
>Mature Secondary Structure 
TMSSVFFEHRIKVRHLRVIEALDRQKSLLRASRVLNVTQPALTRALQEIEEIVGAPLFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHH
RHSRGVRPNAMGEVLVQTAHVVLGQLRRAQDNFEGLLQDDALTITVGALPVAASGLLPAV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
LAALYRTEPTLRVRLLHGRTDELLPKLAGNEIDLVVGRLYPLARYDALIRKVLYQDPIAL
HHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHE
VARSDHPLFANGPVRIAEAAAYRQVLPTLSQHVERDVAQVMREHGLATRDQLRSSSASFT
EECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHH
REILLGTDSIAVMPSMMVAGDIARGELRAFQLQQASQARAGGVIYRDGAAIHKPGVRLLM
HHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
RELENQLALMAEQGAVCVDDLIGEDHVLLDESA
HHHHHHHHHHHHCCCEEHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]