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The map label for this gene is tuf1 [H]

Identifier: 84625058

GI number: 84625058

Start: 3861359

End: 3862549

Strand: Reverse

Name: tuf1 [H]

Synonym: XOO_3401

Alternate gene names: 84625058

Gene position: 3862549-3861359 (Counterclockwise)

Preceding gene: 84625059

Following gene: 84625057

Centisome position: 78.19

GC content: 62.22

Gene sequence:

>1191_bases
ATGGCAAAAGCTAAATTCGAGCGCACCAAGCCGCACGTGAACGTCGGCACCATCGGTCACGTTGACCATGGCAAGACCAC
GTTGACCGCTGCGCTGACCAAGATCGGTGCAGAGCGTTTCGGTGGTGAGTTCAAGGCGTACGACGCGATCGACGCGGCGC
CGGAAGAGAAGGCGCGCGGCATCACGATTTCGACCGCGCACGTCGAATACGAATCCCCGTCGCGCCACTACGCGCACGTG
GATTGCCCCGGTCACGCCGACTATGTCAAGAACATGATCACCGGTGCAGCGCAGATGGACGGCGCGATTCTGGTGTGTTC
GGCCGCGGACGGCCCGATGCCGCAGACCCGTGAGCACATCCTGCTCTCGCGTCAGGTCGGTGTGCCGCACATCGTCGTGT
TCCTGAACAAGGCCGACATGGTCGACGACGCCGAATTGCTCGAGCTAGTCGAAATGGAAGTGCGCGAGTTGCTCAGCAAG
TACGACTTCCCGGGCGACGACACCCCAATCATCCACGGTTCGGCGCGTCTGGCGCTGGATGGCGATCAGAGCGAGATCGG
CGTGCCGGCGATCCTGAAGCTGGTCGACGCGCTCGACACCTTCATCCCGGAGCCGACCCGCGACGTCGATCGTCCGTTCC
TGATGCCGGTGGAAGACGTATTCTCGATCTCGGGCCGCGGCACCGTGGTGACTGGTCGTATCGAGCGCGGCATCATCAAG
GTGGGCGACGAAATCGAAATCGTCGGTATCCGTGCGACGCAGAAGACCACGGTGACGGGCGTGGAAATGTTCCGCAAGCT
GCTTGACCAGGGTCAGGCGGGCGACAACGCGGGTCTGCTGCTGCGCGGCACCAAGCGCGACGACGTCGAGCGTGGCCAGG
TGCTGTGCAAGCCGGGTTCGATCAAGCCGCACACCGAGTTCGAAGCCGAAGTCTACGTGCTGTCGAAGGATGAAGGTGGC
CGTCATACCCCGTTCTTCAAGGGCTACCGTCCGCAGTTGTACTTTCGCACCACCGACATCACGGGCGCGATTGATTTGCC
TGAAGGGGTGGAGATGGTGATGCCTGGCGACAACGTCAAGATGACGGTGACGTTGATCAACCCGGTGGCGATGGACGAAG
GCCTGCGTTTCGCGATCCGCGAAGGCGGCCGTACCGTCGGTGCCGGCGTGGTGTCCAAGATCATCAAGTAA

Upstream 100 bases:

>100_bases
AGCACCTCCTTGGTAAGGAGGAGGTCGAAGGTTCGATTCCTTTCGTGAGCACCATTGCTCAATCGTCTTCAACTCCAAAC
GATTACCGAGACACGCACCC

Downstream 100 bases:

>100_bases
TGCCTGCGTCCAGCCGCTTTATGGTGGTTGGACTCAAACGAATGGCGGGCCGGCTGAACCGCGGTCCGCCTTCGTTGTCT
TAGGGTTTGCGGTAAACGAG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIHGSARLALDGDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
VGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGG
RHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVKMTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIHGSARLALDGDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
VGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGG
RHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVKMTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK
>Mature_395_residues
AKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPSRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIHGSARLALDGDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIKV
GDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGGR
HTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVKMTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=29.0540540540541, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=30.2483069977427, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=28.6666666666667, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=30.2483069977427, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI194018522, Length=442, Percent_Identity=29.185520361991, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018520, Length=442, Percent_Identity=29.185520361991, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194097354, Length=442, Percent_Identity=29.185520361991, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=29.7687861271676, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI46094014, Length=320, Percent_Identity=30.3125, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4503507, Length=357, Percent_Identity=28.0112044817927, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI82546879, Length=432, Percent_Identity=24.7685185185185, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=77.9746835443038, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.9746835443038, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.1005291005291, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=31.6455696202532, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789108, Length=326, Percent_Identity=29.4478527607362, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.1303258145363, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.2196382428941, Blast_Score=347, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=29.5964125560538, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=29.5964125560538, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=29.0993071593533, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=27.1111111111111, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=444, Percent_Identity=26.8018018018018, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.7658862876254, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=444, Percent_Identity=26.8018018018018, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17509919, Length=441, Percent_Identity=25.3968253968254, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=26.1802575107296, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.8291457286432, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=451, Percent_Identity=30.5986696230599, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=451, Percent_Identity=30.5986696230599, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=29.6511627906977, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=459, Percent_Identity=23.3115468409586, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.7310924369748, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.5241730279898, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.5241730279898, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=52.0725388601036, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=28.5714285714286, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=28.5714285714286, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI17137380, Length=340, Percent_Identity=31.4705882352941, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.4754098360656, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45550900, Length=441, Percent_Identity=27.6643990929705, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24647046, Length=434, Percent_Identity=26.4976958525346, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24647048, Length=432, Percent_Identity=26.6203703703704, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43081; Mature: 42950

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCC
ITISTAHVEYESPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALD
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEC
GDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEE
VGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGS
ECCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCC
IKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVK
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCEE
MTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCC
ITISTAHVEYESPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALD
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEC
GDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEE
VGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGS
ECCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCC
IKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVK
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCEE
MTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA