Click here to switch to the map view.

The map label for this gene is dosP [H]

Identifier: 84624372

GI number: 84624372

Start: 3059421

End: 3061664

Strand: Direct

Name: dosP [H]

Synonym: XOO_2715

Alternate gene names: 84624372

Gene position: 3059421-3061664 (Clockwise)

Preceding gene: 84624371

Following gene: 84624377

Centisome position: 61.93

GC content: 63.06

Gene sequence:

>2244_bases
GTGCATGCGGACACGCCAAGCCACGAGCTGGTCAGACCCGGCCCGGTCACCGCTGGCATGGCCAAGGCCGGCGAACAGCC
TGAGCAACGCCGTTCGCGCCTGTTGGCCGAAGCACTGGATGGCAGCAGCAGCGCCATTCTCATCTGCGATCTGCAATCGC
GCCTGCTGTATGCCAACGACGGCTTCCAGCGCATGTTCGGCTACACCGAAGCCGAGCTGGTCGGTCAGCGTCCATCGGAT
GTCTTGACCCGCGCGCAAAGCGACCAAAGCGAAGTCGCCCGTGTGCGCGATCGCGCCGATGCCTTGCAGCACACCCGCGC
CGAACTGCTGGTGTACCGCAAGGACGGCACGCCGCTATGGATCTCGCTCAATTTCAACCCAATCTACGACGCCGACCACA
CCCCCTTGCATTACGTGGCGGTGCTGACCGACATCACCGACACCAAGATGCACGAGGTATTGCAGCACCGCGTGCTGGAA
GCCTTGCTGCAGGAACGCCCGTTGATCGAGGTGATGACGCTGATTTGCACCGAGGTCGAACGCATTGCACCGGACGTGCT
GGCCGCGGTGACGAGTGCGGACAATCAAGGCCGCCTGCATTCGCTGGCGGCACCTAGCCTGCCACCCGAGTACGCCGATG
CGGTCAATGGTTTGAAGATCGGCCCGGGCGCAGGCGCCTGCGGCACCGCGGCCTGGCGAGGTCGCGCGGTGATGGTGGAA
GACATCGCGACCGACCCGCTGTTCGAACCATGTCGCGAAACGATGTTGCGCTGGGACCTGCACGCCTGCTGGAGTACGCC
GATCCGCTCCAACAGCGCGCGCGTGCTCGGCACCTTCGCGCTGTATTACCGCAAGCCGCAACATGCCAATGCCTGGCATA
TGCGGCTGACCGATTTGTGCCAGCAACTGTGCACGTTGGCGCTGGAGCGCGAGCAGATCCGCCAGCGCGTGCATCAACTG
GCGTTCTACGACACGCTCACCGGTTTGCCGAATCGCATCATGTTCAGCGCGCGTGCCGAGCAGGCGCTGACGCAGGCCGA
ATACGCCGGCGAACCGGCAGCGTTGGTGTTCATCAACATCGATCGTTTCAAACTCATCAACGATAGCCAGGGTCATGCCG
CAGGCGACGGTCTGTTGCGCGATATCGCCCTACGCATCAACGATCAACTCACTGCCACAGCGATGCTGGCGCGCCTGTCA
GGCGACGAATTCGCGCTTGCGCTGCCGCAATGCAGCGCAGAACAGGCCAGTGCCGTCGCCGAGCGTGTGTTGGTCAGCAT
CGCCAACCCGTTGCCGGTTGGCCATATGACCGTGCATCCCTCGGCCAGTATCGGCGTGGCGATGTTTCCGGAGGACGGCC
GCGAGATCAACATCCTGTTGCGCCACGCCGATCTGGCGATGAATCGCGCCAAGAAGGAAGGCGGCGGGCGTTTCCGCTTC
TTCAGCGCCGACATGAACCGCATGGCGCAAGAACGCGTTGCGCTGGAAGCGGAGCTGCGCCAGGCCATCGGCCGCGATCA
GCTGCAACTACATTACCAGCCGCAAATACACAGCGCCGCGCCGCACACGCTGTATGGCGTGGAAGCGCTACTGCGCTGGA
ATCATCCGCAATTGGGCGCGGTGTCGCCGGCACAATTCTTACAGGTGGCCGAAGAGCAAGGCATGATGCCGCAGATCAAT
GCGTGGGTACTGCGCAGCGCCTGCCAGCAGGTCGCCGACTGGCGCCAGCGTGGCGTGCCGGTGGCACGTGTCTCGGTGAA
TCTGTCGGCAAGCGGTTTCGAAAGTCCACGCATGCTGTCGGATTTGCTCCAACTTATCGCCGACATGGGCGTAAAGCCCT
CCGACCTGACCCTGGAACTGACCGAAAGCGTGATGCTGTCCGGGCAACCGGGCGTGCTGGAAAACCTGCACGCCATTCGC
GAAGCCGGTATCTCGCTGTCGCTGGACGATTTCGGCACCGGCTATTCCAGTCTTAGCCATTTGCATCGCTTGCCGATCGA
CGAACTCAAGCTGGACATGAGCTTCGTCCGCGATATCGAACACAGCGAAGACGCGCGTGCACTCATCACCTCGGTGCTGC
GCATCGGCGAGCACCTGCGCAAGCACGTGGTCGCCGAAGGCGTGGAAAACGAAGCACAACGCCTGTTTCTCGCCGACCTG
GGCTGCGAGGTCTTACAAGGCTACCTGTTCTCGCGACCGCTGCCGGCCGCAGCGCTGGAAGCTTGGTTGGGCGCGCAGCC
TTAG

Upstream 100 bases:

>100_bases
GGCCGCGCATCCTTCGCACCCGAGCGACCCGACGCTTCCACTGACACCGGGAAGCGCGACGGACGCGGCGGCCGATCGCA
CGCCTGGGACGGCTGAACCG

Downstream 100 bases:

>100_bases
CGCGTACAACAAAACGACTGCGCGGCCGGTGCTCGGAATCCTCATGCAACGCGATTGCGGCATGGATGCTGCGCACTCGC
GTTCCCAGAGCGGCTAACAA

Product: c-di-GMP phosphodiesterase A

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MHADTPSHELVRPGPVTAGMAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGQRPSD
VLTRAQSDQSEVARVRDRADALQHTRAELLVYRKDGTPLWISLNFNPIYDADHTPLHYVAVLTDITDTKMHEVLQHRVLE
ALLQERPLIEVMTLICTEVERIAPDVLAAVTSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGAGACGTAAWRGRAVMVE
DIATDPLFEPCRETMLRWDLHACWSTPIRSNSARVLGTFALYYRKPQHANAWHMRLTDLCQQLCTLALEREQIRQRVHQL
AFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALVFINIDRFKLINDSQGHAAGDGLLRDIALRINDQLTATAMLARLS
GDEFALALPQCSAEQASAVAERVLVSIANPLPVGHMTVHPSASIGVAMFPEDGREINILLRHADLAMNRAKKEGGGRFRF
FSADMNRMAQERVALEAELRQAIGRDQLQLHYQPQIHSAAPHTLYGVEALLRWNHPQLGAVSPAQFLQVAEEQGMMPQIN
AWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLSDLLQLIADMGVKPSDLTLELTESVMLSGQPGVLENLHAIR
EAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALITSVLRIGEHLRKHVVAEGVENEAQRLFLADL
GCEVLQGYLFSRPLPAAALEAWLGAQP

Sequences:

>Translated_747_residues
MHADTPSHELVRPGPVTAGMAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGQRPSD
VLTRAQSDQSEVARVRDRADALQHTRAELLVYRKDGTPLWISLNFNPIYDADHTPLHYVAVLTDITDTKMHEVLQHRVLE
ALLQERPLIEVMTLICTEVERIAPDVLAAVTSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGAGACGTAAWRGRAVMVE
DIATDPLFEPCRETMLRWDLHACWSTPIRSNSARVLGTFALYYRKPQHANAWHMRLTDLCQQLCTLALEREQIRQRVHQL
AFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALVFINIDRFKLINDSQGHAAGDGLLRDIALRINDQLTATAMLARLS
GDEFALALPQCSAEQASAVAERVLVSIANPLPVGHMTVHPSASIGVAMFPEDGREINILLRHADLAMNRAKKEGGGRFRF
FSADMNRMAQERVALEAELRQAIGRDQLQLHYQPQIHSAAPHTLYGVEALLRWNHPQLGAVSPAQFLQVAEEQGMMPQIN
AWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLSDLLQLIADMGVKPSDLTLELTESVMLSGQPGVLENLHAIR
EAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALITSVLRIGEHLRKHVVAEGVENEAQRLFLADL
GCEVLQGYLFSRPLPAAALEAWLGAQP
>Mature_747_residues
MHADTPSHELVRPGPVTAGMAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGQRPSD
VLTRAQSDQSEVARVRDRADALQHTRAELLVYRKDGTPLWISLNFNPIYDADHTPLHYVAVLTDITDTKMHEVLQHRVLE
ALLQERPLIEVMTLICTEVERIAPDVLAAVTSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGAGACGTAAWRGRAVMVE
DIATDPLFEPCRETMLRWDLHACWSTPIRSNSARVLGTFALYYRKPQHANAWHMRLTDLCQQLCTLALEREQIRQRVHQL
AFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALVFINIDRFKLINDSQGHAAGDGLLRDIALRINDQLTATAMLARLS
GDEFALALPQCSAEQASAVAERVLVSIANPLPVGHMTVHPSASIGVAMFPEDGREINILLRHADLAMNRAKKEGGGRFRF
FSADMNRMAQERVALEAELRQAIGRDQLQLHYQPQIHSAAPHTLYGVEALLRWNHPQLGAVSPAQFLQVAEEQGMMPQIN
AWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLSDLLQLIADMGVKPSDLTLELTESVMLSGQPGVLENLHAIR
EAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALITSVLRIGEHLRKHVVAEGVENEAQRLFLADL
GCEVLQGYLFSRPLPAAALEAWLGAQP

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=721, Percent_Identity=29.126213592233, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1787541, Length=462, Percent_Identity=35.9307359307359, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI226510982, Length=451, Percent_Identity=30.3769401330377, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1790496, Length=264, Percent_Identity=32.1969696969697, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI87081845, Length=263, Percent_Identity=31.1787072243346, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788502, Length=249, Percent_Identity=31.7269076305221, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081743, Length=244, Percent_Identity=33.6065573770492, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1787055, Length=258, Percent_Identity=29.0697674418605, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788849, Length=447, Percent_Identity=25.7270693512304, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI87081980, Length=263, Percent_Identity=32.319391634981, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1786507, Length=239, Percent_Identity=30.9623430962343, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788381, Length=434, Percent_Identity=26.7281105990783, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI87082096, Length=456, Percent_Identity=24.780701754386, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693134, Length=218, Percent_Identity=29.3577981651376, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI87081977, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=34.1317365269461, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=29.4416243654822, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=30.2469135802469, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787262, Length=229, Percent_Identity=26.2008733624454, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787410, Length=150, Percent_Identity=28.6666666666667, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=134, Percent_Identity=37.3134328358209, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=28.6585365853659, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787802, Length=206, Percent_Identity=27.1844660194175, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789650, Length=478, Percent_Identity=20.5020920502092, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 82462; Mature: 82462

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHADTPSHELVRPGPVTAGMAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYAND
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCC
GFQRMFGYTEAELVGQRPSDVLTRAQSDQSEVARVRDRADALQHTRAELLVYRKDGTPLW
CHHHHHCCCHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEE
ISLNFNPIYDADHTPLHYVAVLTDITDTKMHEVLQHRVLEALLQERPLIEVMTLICTEVE
EEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RIAPDVLAAVTSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGAGACGTAAWRGRAVMVE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCHHHCCCEEEEE
DIATDPLFEPCRETMLRWDLHACWSTPIRSNSARVLGTFALYYRKPQHANAWHMRLTDLC
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
QQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALVFINI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHCCCCEEEEEEEE
DRFKLINDSQGHAAGDGLLRDIALRINDQLTATAMLARLSGDEFALALPQCSAEQASAVA
CEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHH
ERVLVSIANPLPVGHMTVHPSASIGVAMFPEDGREINILLRHADLAMNRAKKEGGGRFRF
HHHHHHHCCCCCCCEEEECCCCCCCEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEE
FSADMNRMAQERVALEAELRQAIGRDQLQLHYQPQIHSAAPHTLYGVEALLRWNHPQLGA
EHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
VSPAQFLQVAEEQGMMPQINAWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHH
DLLQLIADMGVKPSDLTLELTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSH
HHHHHHHHCCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHCCCEEEEHHCCCCHHHHHH
LHRLPIDELKLDMSFVRDIEHSEDARALITSVLRIGEHLRKHVVAEGVENEAQRLFLADL
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GCEVLQGYLFSRPLPAAALEAWLGAQP
HHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MHADTPSHELVRPGPVTAGMAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYAND
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCC
GFQRMFGYTEAELVGQRPSDVLTRAQSDQSEVARVRDRADALQHTRAELLVYRKDGTPLW
CHHHHHCCCHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEE
ISLNFNPIYDADHTPLHYVAVLTDITDTKMHEVLQHRVLEALLQERPLIEVMTLICTEVE
EEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RIAPDVLAAVTSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGAGACGTAAWRGRAVMVE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCHHHCCCEEEEE
DIATDPLFEPCRETMLRWDLHACWSTPIRSNSARVLGTFALYYRKPQHANAWHMRLTDLC
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
QQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALVFINI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHCCCCEEEEEEEE
DRFKLINDSQGHAAGDGLLRDIALRINDQLTATAMLARLSGDEFALALPQCSAEQASAVA
CEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHH
ERVLVSIANPLPVGHMTVHPSASIGVAMFPEDGREINILLRHADLAMNRAKKEGGGRFRF
HHHHHHHCCCCCCCEEEECCCCCCCEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEE
FSADMNRMAQERVALEAELRQAIGRDQLQLHYQPQIHSAAPHTLYGVEALLRWNHPQLGA
EHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
VSPAQFLQVAEEQGMMPQINAWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHH
DLLQLIADMGVKPSDLTLELTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSH
HHHHHHHHCCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHCCCEEEEHHCCCCHHHHHH
LHRLPIDELKLDMSFVRDIEHSEDARALITSVLRIGEHLRKHVVAEGVENEAQRLFLADL
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GCEVLQGYLFSRPLPAAALEAWLGAQP
HHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]