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The map label for this gene is fruB [H]

Identifier: 84624307

GI number: 84624307

Start: 2968889

End: 2971405

Strand: Direct

Name: fruB [H]

Synonym: XOO_2650

Alternate gene names: 84624307

Gene position: 2968889-2971405 (Clockwise)

Preceding gene: 84624305

Following gene: 84624308

Centisome position: 60.1

GC content: 70.12

Gene sequence:

>2517_bases
TTGTCCTCTCCGTCGCTCGCCCCCGTCAACCCCGACCTTGTGCGCCTGCGCGCGTCTGTCCGCGACAAGGACGACGCCAT
CGCTCAGGCTGCCCAGCTGCTGGTCGCGGCCGGCTGCGTGGCACCTGGCTACGACGCCAGCATGCGGCGCCGCGAATGCC
TGGCGAACACCTTTCTGGGCCACGGCCTGGCGATCCCGCACGGCGTCGTCGAAGACCGCCACCTGGTGCTTCGCGACGGT
ATCGCGGTGCTGCAGCTGCCCGAGGGAGTGGAATGGAACCCCGGCCAGACCACCCGGCTGGTGGTCGGCATCGCCGCGCA
GTCCGATACCCACATCACCTTGCTGCGCCGGCTGACCCGGTTGATCCAGGACCCGGCGCAGCTGGAAGCGCTGTTCAGCA
CCGACGACCCCGGCGTGATCGTCGCCGCACTCACCGGCGACCGGGCACCCGACACCAGCGCCGCGCCAGCCACCGATCTG
GCCGAAACATTCGAGTGGACCGTTGCTTATCCCAGCGGCCTGCACGCCCGTCCGGCCACCCGCTGGGCCGAGACCGCCCG
CGGCTTCTCGGCCCGCGCGCAGGTCCGCGCCGGCGACCAGGCCGCCGATGCCAAGAGCCTGGTCGGCCTGCTGCAGCTGG
GCCTGCGTGCCGGCGACAGCATCACCGTCTCGGCCGAGGGCAGCGACGCGGCGGCGCTGCTCAAGTGTCTGCGCGTGGTG
ATGGACAGCCTCACCGCGCAGGAAAAGGCCGATGCCGAGCGTGCCGCACAACGCCGCGCCGCCCCGGTGGCCGGCTGGAC
CCCGCCGCAGGCGCAGCCGGCCATCGTCGGCATCGGCGCCAGCCCGGGCGTGGCGATCGGCATCGTGCATCGCCTGCGTG
CGGCGCAGACCGAAGTGGCCGATCAACCGGTCGGCCTGGGCGATGGCGGCGCGCAGCTGCACGATGCGCTGACCCGTACC
CGCCAGCAGCTGGCGGCGATCCAGGACGACACCCAGCGCCGGCTCGGCGCCTCGGACGCGGCCATCTTCAAGGCCCAGGC
CGAACTGCTCAACGACAACGACCTGATCACCCGCACCTGCCAGCTGATGGTCGAAGGCCACGGCGTGGCATGGTCGTGGC
ACCAGGCGGTGGAACAGATTGCCTCCGGCCTGGCCGCTCTCGGCAACCCCGTGCTGGCCGGCCGCGCTGCCGATCTACGC
GATGTCGGTCGCCGCGTGCTAGCGCAGCTCGACCCGACCGCCGCTGGCGCCGGTCTGACCGACCTGCCCGCGCAGCCGTG
CATCCTGCTCGCCAGCGATCTGTCGCCGTCGGACACCGCCAATCTGGACACCGCGCGCGTGCTCGGCCTGGCCACCGCGC
AGGGCGGGCCGACCTCGCACACCGCAATTCTGTCGCGCACGCTCGGCCTGCCGGCGCTGGTCGCGGCCGGTGGCCAGCTG
CTGGATATCGAAGACGGCGTCACTGCCATCATCGACGGCAGCAGCGGGCGCCTGTACCTCAATCCGTCAGAACTCGATCT
GGACGCCGCACGCACGCATATCGCCGAACAGCAGGCGATTCGCGAGCGCGAAGCCGCGCAACGCGCATTGCCGGCCGAAA
CCACCGACGGCCACCACATCGACATTGGCGCCAACGTCAATCTGCCCGACCAGGTCGCGATGGCGCTGACCCAGGGCGCC
GAAGGCGTCGGCCTGATGCGCACCGAATTCCTGTTCCTGGAAAGCGGACGCACCCCCAGCGAAGACGAACAGCATGCCAC
CTATCTGGCAATGGCGCAGGCACTGGATGGCCGCCCGCTGATCGTGCGTGCGCTGGATATCGGTGGCGACAAGCAGGTGG
CGCATCTGGAATTGCCGCACGAAGAAAACCCGTTCCTGGGTGTGCGCGGCGCGCGCTTGCTGCTGCGTCGTCCCGACCTG
CTGGAGCCGCAACTGCGTGCGCTGTATCGCGCCGCCAAGGACGGTGCGCGGCTGTCGATCATGTTCCCGATGATCACCTC
GGTGCCGGAGCTGATCGCCTTGCGCGCCATCTGCGCACGCATCCGCGCCGAGCTCGACGCACCGGAAGTGCCGATCGGCA
TCATGATCGAAGTGCCGGCTGCCGCCGCACAGGCCGACGTACTGGCGCGCCACGCAGACTTCTTCTCGATCGGCACCAAC
GACCTTACCCAGTACGTGCTGGCGATCGACCGCCAGAATTCGGAACTGGCCGCTGAAGCCGACAGCCTGCACCCGGCCGT
GCTGCGCATGATCCGCAGCACCATTGAAGGTGCGCGCACACACGAGCGCTGGGTCGGCGTGTGCGGCGGTCTGGCCGGCG
ACGCCTTCGGCGCCAGCCTGCTGGCCGGCCTGGGCGTGCAGGAATTGTCGATGACGCCAAACGACATCCCCGCGGTGAAG
GCTCGCCTGCGCGGCGCGGCGCTGCGCCAGTTGCAGGAACTGGCCGAACAGGCGCTGGCCTGCGAAACCGCCGAACAGGT
GCGCGCATTGGAAGCCAAGCGCGAGGACGCGGCATGA

Upstream 100 bases:

>100_bases
AACCCGAAAGCAATACAACGGTTTTGTGCAGTGCACATTGACGTAAATTGTGGGAACGTTCCCACTACACTTTCCAGCCC
GAACTCATCCGGAGCCCGCC

Downstream 100 bases:

>100_bases
GCCTGCAAGCCATCACCGTCACGCTGAATCCGGCGATCGACCAGACCATCCAGCTGGAACATCTGCAGCCTGGCGCAGTG
CATCGCGCCAGCAGCGTGCG

Product: multiphosphoryl transfer protein

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MSSPSLAPVNPDLVRLRASVRDKDDAIAQAAQLLVAAGCVAPGYDASMRRRECLANTFLGHGLAIPHGVVEDRHLVLRDG
IAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFSTDDPGVIVAALTGDRAPDTSAAPATDL
AETFEWTVAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAALLKCLRVV
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPVGLGDGGAQLHDALTRT
RQQLAAIQDDTQRRLGASDAAIFKAQAELLNDNDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLR
DVGRRVLAQLDPTAAGAGLTDLPAQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LDIEDGVTAIIDGSSGRLYLNPSELDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPDQVAMALTQGA
EGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDL
LEPQLRALYRAAKDGARLSIMFPMITSVPELIALRAICARIRAELDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNSELAAEADSLHPAVLRMIRSTIEGARTHERWVGVCGGLAGDAFGASLLAGLGVQELSMTPNDIPAVK
ARLRGAALRQLQELAEQALACETAEQVRALEAKREDAA

Sequences:

>Translated_838_residues
MSSPSLAPVNPDLVRLRASVRDKDDAIAQAAQLLVAAGCVAPGYDASMRRRECLANTFLGHGLAIPHGVVEDRHLVLRDG
IAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFSTDDPGVIVAALTGDRAPDTSAAPATDL
AETFEWTVAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAALLKCLRVV
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPVGLGDGGAQLHDALTRT
RQQLAAIQDDTQRRLGASDAAIFKAQAELLNDNDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLR
DVGRRVLAQLDPTAAGAGLTDLPAQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LDIEDGVTAIIDGSSGRLYLNPSELDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPDQVAMALTQGA
EGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDL
LEPQLRALYRAAKDGARLSIMFPMITSVPELIALRAICARIRAELDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNSELAAEADSLHPAVLRMIRSTIEGARTHERWVGVCGGLAGDAFGASLLAGLGVQELSMTPNDIPAVK
ARLRGAALRQLQELAEQALACETAEQVRALEAKREDAA
>Mature_837_residues
SSPSLAPVNPDLVRLRASVRDKDDAIAQAAQLLVAAGCVAPGYDASMRRRECLANTFLGHGLAIPHGVVEDRHLVLRDGI
AVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFSTDDPGVIVAALTGDRAPDTSAAPATDLA
ETFEWTVAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAALLKCLRVVM
DSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPVGLGDGGAQLHDALTRTR
QQLAAIQDDTQRRLGASDAAIFKAQAELLNDNDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRD
VGRRVLAQLDPTAAGAGLTDLPAQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQLL
DIEDGVTAIIDGSSGRLYLNPSELDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPDQVAMALTQGAE
GVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLL
EPQLRALYRAAKDGARLSIMFPMITSVPELIALRAICARIRAELDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTND
LTQYVLAIDRQNSELAAEADSLHPAVLRMIRSTIEGARTHERWVGVCGGLAGDAFGASLLAGLGVQELSMTPNDIPAVKA
RLRGAALRQLQELAEQALACETAEQVRALEAKREDAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=572, Percent_Identity=39.1608391608392, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788726, Length=684, Percent_Identity=31.5789473684211, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI48994992, Length=704, Percent_Identity=32.2443181818182, Blast_Score=289, Evalue=5e-79,
Organism=Escherichia coli, GI1789193, Length=499, Percent_Identity=35.6713426853707, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1788494, Length=127, Percent_Identity=46.4566929133858, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787994, Length=431, Percent_Identity=25.2900232018561, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI226510935, Length=331, Percent_Identity=26.5861027190332, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790027, Length=149, Percent_Identity=34.8993288590604, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88351; Mature: 88220

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSLAPVNPDLVRLRASVRDKDDAIAQAAQLLVAAGCVAPGYDASMRRRECLANTFLG
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HGLAIPHGVVEDRHLVLRDGIAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTR
CCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
LIQDPAQLEALFSTDDPGVIVAALTGDRAPDTSAAPATDLAETFEWTVAYPSGLHARPAT
HHCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCHH
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAALLKCLRVV
HHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
DQPVGLGDGGAQLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDNDLITRTC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHH
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPTAAGAGLT
HHHEECCCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
DLPAQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
CCCCCCEEEEECCCCCCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCE
LDIEDGVTAIIDGSSGRLYLNPSELDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
EEECCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DIGANVNLPDQVAMALTQGAEGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPL
EECCCCCCHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCE
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
EEEEEECCCCCEEEEEECCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
MFPMITSVPELIALRAICARIRAELDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCH
DLTQYVLAIDRQNSELAAEADSLHPAVLRMIRSTIEGARTHERWVGVCGGLAGDAFGASL
HHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LAGLGVQELSMTPNDIPAVKARLRGAALRQLQELAEQALACETAEQVRALEAKREDAA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSPSLAPVNPDLVRLRASVRDKDDAIAQAAQLLVAAGCVAPGYDASMRRRECLANTFLG
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HGLAIPHGVVEDRHLVLRDGIAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTR
CCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
LIQDPAQLEALFSTDDPGVIVAALTGDRAPDTSAAPATDLAETFEWTVAYPSGLHARPAT
HHCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCHH
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAALLKCLRVV
HHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
DQPVGLGDGGAQLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDNDLITRTC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHH
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPTAAGAGLT
HHHEECCCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
DLPAQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
CCCCCCEEEEECCCCCCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCE
LDIEDGVTAIIDGSSGRLYLNPSELDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
EEECCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DIGANVNLPDQVAMALTQGAEGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPL
EECCCCCCHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCE
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
EEEEEECCCCCEEEEEECCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
MFPMITSVPELIALRAICARIRAELDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCH
DLTQYVLAIDRQNSELAAEADSLHPAVLRMIRSTIEGARTHERWVGVCGGLAGDAFGASL
HHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LAGLGVQELSMTPNDIPAVKARLRGAALRQLQELAEQALACETAEQVRALEAKREDAA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]