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The map label for this gene is fliA [H]

Identifier: 84624137

GI number: 84624137

Start: 2760325

End: 2761080

Strand: Direct

Name: fliA [H]

Synonym: XOO_2480

Alternate gene names: 84624137

Gene position: 2760325-2761080 (Clockwise)

Preceding gene: 84624136

Following gene: 84624138

Centisome position: 55.87

GC content: 64.95

Gene sequence:

>756_bases
ATGAACACCACCGCCGCAGCGCAGTACCGTGCCGTCCAGCGCAACAACGCCGACAACGTGGTAACCCAGCACGCCGACCT
GGTTCGCCGCATCGCCCACCACCTGGCCGCGCGTCTGCCGGCCAGCGTGGAAGTGGACGATCTGATCCAGGCTGGCATGA
TCGGCCTGATCGAGGCTTCGCGCAGCTATGACTCCGAACAGGGCGCCTCGTTCGAAACCTACGCCTCGATCCGTATCCGC
GGTTCGATGATCGACGAAATCCGCCGCGGCGACTGGGTGCCGCGCTCGGTGCACCGCCGCGCCCGCGATGCCGCTGCCGC
TGTGCGCAAGATCGAACAGAACACCGGCCGCGCCGCTGCCGCCGCCGAAGTGGCCGCCGCAATGGAAATGCCGCTGCCCG
ATTACCTGCGTCTGATGGAAGACGCCGCACGCGGCCAGGTGCTGAGCCTGGAATCGCGCATCGAAGATCACGGCGAGCTG
GACACCACCGCCAAGGGTGGCCCCAACCCGCAGCAGATGATGGAACGTAGCGAATTCGGCCGCGAACTGGGCAAGGCCAT
CGGCCAATTGCCCGAGCGCGAGCAGCTGGTGCTGTCGCTGTACTACGAACAGGAATTGAATCTGAAAGAAATCGGCGCCG
TGCTCGGTGTCAGCGAATCGCGCGTCTGCCAGATCCACGGCCAGGCGGTGGTGCGTTTGCGGGGCCGCCTCAAAGTCTTC
GAACTGGCCGATGCCGGTGTCGAAATTGACGAATAA

Upstream 100 bases:

>100_bases
CCGAGATCGCACGTCGCACATCGCGCTGGCAGGCTCCCACCGTGCCGCGCGGCAATGTCGAGTTTTTCGTCGAACGCATC
ATCCAACGGGGGGTTGCCGC

Downstream 100 bases:

>100_bases
TTTTTCGCGTATTTTTTCCTGTTAGGAGCTTGATGTGAACAAGAACATGCGGATCCTGATCGTGGACGACTTCTCGACGA
TGCGACGTATCGTCAAGAAT

Product: RNA polymerase sigma factor FliA

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNTTAAAQYRAVQRNNADNVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYASIRIR
GSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAAAEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGEL
DTTAKGGPNPQQMMERSEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRLKVF
ELADAGVEIDE

Sequences:

>Translated_251_residues
MNTTAAAQYRAVQRNNADNVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYASIRIR
GSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAAAEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGEL
DTTAKGGPNPQQMMERSEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRLKVF
ELADAGVEIDE
>Mature_251_residues
MNTTAAAQYRAVQRNNADNVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYASIRIR
GSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAAAEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGEL
DTTAKGGPNPQQMMERSEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRLKVF
ELADAGVEIDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=50.6912442396313, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27684; Mature: 27684

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTAAAQYRAVQRNNADNVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEAS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RSYDSEQGASFETYASIRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAA
HCCCCCCCCCEEHEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
AAEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGELDTTAKGGPNPQQMMERSEFG
HHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
RELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRLKVF
HHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCEEE
ELADAGVEIDE
EECCCCCCCCC
>Mature Secondary Structure
MNTTAAAQYRAVQRNNADNVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEAS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RSYDSEQGASFETYASIRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAA
HCCCCCCCCCEEHEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
AAEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGELDTTAKGGPNPQQMMERSEFG
HHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
RELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRLKVF
HHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCEEE
ELADAGVEIDE
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]