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The map label for this gene is flhF [H]

Identifier: 84624135

GI number: 84624135

Start: 2757799

End: 2759457

Strand: Direct

Name: flhF [H]

Synonym: XOO_2478

Alternate gene names: 84624135

Gene position: 2757799-2759457 (Clockwise)

Preceding gene: 84624134

Following gene: 84624136

Centisome position: 55.82

GC content: 69.08

Gene sequence:

>1659_bases
ATGAAAATCAAACGCTTCGTTGCCCCGGACATGCGCACCGCCTTCCGCATGGTGCGTGAAGAACACGGCCCGGACGCAGT
GATCCTGTCCAACCGCCGTACCGCCGAAGGCATCGAGATCGTCGCTGCCAGCAACTACGACGAAGAACTCGTGCAGCGCG
CCCTGGAAACCGCGCGCTCCGAGACATCAGGACGCGCACATAATGCGGTGGGCCCCAATCAGCAGGCGCCTGCCCCGCAA
GCCCCGACCAAGCCTGCCGCGCCGGTGCATGCCCCGCTCAAGCTGGCTGCCAACGCCAACGTGAGCCAGCGCCAGCGCGT
TGCCAACGCCGCTGAAGACATGATCGCCGCCATGGCGCTGCGTCAGCCGGTCAACGTGCCGCGCCAGGCGCCGGCCGCCG
CACCGGTGCGCACCGCATCCATCCCCTCGCCGGCCGCCCAGGCATTGGCGCACGCGGTGGCCGTCACCACCGCACCGCGC
CAGGAACACGCGCTGTCGGCCGTGCCGGAACAGCTGTTCGCCGACTTTTTGACCACCGCTCCGATGCAGGCTCCGGTGCA
GGCTCCGGTCCACGCTGCCCCGGTGCAGGCACGCACGCCGATCATGGCCGCCGCGCTTGCCACCCACGCCTCTTACGGGC
AGGACGACGACGAGCAGGACGACGACGGCTTCGACATGGACGACGCGCTGCCGCCGATCGTGGTCGCCCCGGCCGCCGTG
GCGGTCGCTGCTGCCGCTCCGGCGCCGCAGAACGACGAAGAACTCAAGCAGCTGCGCGGTGAGCTGGCGCTGATGCGGCA
GATGATCGAGCGCGAAATGAACCGCCTCACCGACGAGCGCCTGCGTGGCTCACCGGCGCGTGCGCAGGCGCTGGAGCTGA
TGGACGACTACGGCTTCGATGCCGGCTTGACCCGCGATGTCGCCATGCAGATCCCGGCCGATACCGAATTGCACCGTGGC
CGCGGCCTGATGCTGGGCCTGCTGTCCAAGCGTCTGCCGGTGGCCCCGGTCGACCCGCTGGAACGCGGCGGCGTGATCGC
GTTGGTCGGCCCGACCGGCGCCGGCAAGACCACCACCATTGCCAAACTCGCCCAGCGCTTCGCCGCACAGCACACTCCGC
GCGACGTGGCCCTGGTCACCACCGACACCCAGCGCGTCGGCGGCCGCGAGCAGCTGCACAGCTACGGCCGTCAGCTCGGC
GTCGCGGTGCACGAAGCCGACAGCGCCGAAAGCCTGCTTGACCTGTTCGAGCGCCTGCGCGACTACAAGCTGGTGCTGAT
CGACACCGCCGGCATGGGCCAGCGCGACCGCGCCCTGGCCGCCCAGCTGAACTGGCTGCGCGCCGCCCAGCAGGTCACCT
CGCTGCTGGTGCTGCCTGCCAACGCCCATTTTTCCGACCTCGACGAGGTCGTACGCCGCTTCGCCCACGCCAAACCCCAA
GGCGTGGTGCTGACCAAACTCGACGAGACCGGCCGCTTCGGCAGCGCCTTGTCGGTGGTGGTCGACCACCAAATGCCCAT
CACCTGGGTGACTGACGGACAGCGGGTCCCCGACGACCTGCACCGCGCCAATGCCGCCAGTCTCGTTCTTCGCCTTGAAG
ATTTGCGGCGCGCTGCCGATAAGCCCTGTACTCCGGAGCACAACCATGCAGTCGCGTGA

Upstream 100 bases:

>100_bases
ACAACGACCGGGACAGGCACCTCATGACGCTGGCAACACACCCACATTCATCGCGCACCCGTCCGGCGTCCGGCGGGTCC
CGTTCCTCGAGGCAATTCGC

Downstream 100 bases:

>100_bases
ATACGCCAAGCTGACCAACGCCTTCCCCCTGTCGGCGACCCGTCCCGAGCCTTTGGGCCCGGTGCGCACCATTGCCGTGA
CCGGTGGCAAGGGTGGCGTG

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKIKRFVAPDMRTAFRMVREEHGPDAVILSNRRTAEGIEIVAASNYDEELVQRALETARSETSGRAHNAVGPNQQAPAPQ
APTKPAAPVHAPLKLAANANVSQRQRVANAAEDMIAAMALRQPVNVPRQAPAAAPVRTASIPSPAAQALAHAVAVTTAPR
QEHALSAVPEQLFADFLTTAPMQAPVQAPVHAAPVQARTPIMAAALATHASYGQDDDEQDDDGFDMDDALPPIVVAPAAV
AVAAAAPAPQNDEELKQLRGELALMRQMIEREMNRLTDERLRGSPARAQALELMDDYGFDAGLTRDVAMQIPADTELHRG
RGLMLGLLSKRLPVAPVDPLERGGVIALVGPTGAGKTTTIAKLAQRFAAQHTPRDVALVTTDTQRVGGREQLHSYGRQLG
VAVHEADSAESLLDLFERLRDYKLVLIDTAGMGQRDRALAAQLNWLRAAQQVTSLLVLPANAHFSDLDEVVRRFAHAKPQ
GVVLTKLDETGRFGSALSVVVDHQMPITWVTDGQRVPDDLHRANAASLVLRLEDLRRAADKPCTPEHNHAVA

Sequences:

>Translated_552_residues
MKIKRFVAPDMRTAFRMVREEHGPDAVILSNRRTAEGIEIVAASNYDEELVQRALETARSETSGRAHNAVGPNQQAPAPQ
APTKPAAPVHAPLKLAANANVSQRQRVANAAEDMIAAMALRQPVNVPRQAPAAAPVRTASIPSPAAQALAHAVAVTTAPR
QEHALSAVPEQLFADFLTTAPMQAPVQAPVHAAPVQARTPIMAAALATHASYGQDDDEQDDDGFDMDDALPPIVVAPAAV
AVAAAAPAPQNDEELKQLRGELALMRQMIEREMNRLTDERLRGSPARAQALELMDDYGFDAGLTRDVAMQIPADTELHRG
RGLMLGLLSKRLPVAPVDPLERGGVIALVGPTGAGKTTTIAKLAQRFAAQHTPRDVALVTTDTQRVGGREQLHSYGRQLG
VAVHEADSAESLLDLFERLRDYKLVLIDTAGMGQRDRALAAQLNWLRAAQQVTSLLVLPANAHFSDLDEVVRRFAHAKPQ
GVVLTKLDETGRFGSALSVVVDHQMPITWVTDGQRVPDDLHRANAASLVLRLEDLRRAADKPCTPEHNHAVA
>Mature_552_residues
MKIKRFVAPDMRTAFRMVREEHGPDAVILSNRRTAEGIEIVAASNYDEELVQRALETARSETSGRAHNAVGPNQQAPAPQ
APTKPAAPVHAPLKLAANANVSQRQRVANAAEDMIAAMALRQPVNVPRQAPAAAPVRTASIPSPAAQALAHAVAVTTAPR
QEHALSAVPEQLFADFLTTAPMQAPVQAPVHAAPVQARTPIMAAALATHASYGQDDDEQDDDGFDMDDALPPIVVAPAAV
AVAAAAPAPQNDEELKQLRGELALMRQMIEREMNRLTDERLRGSPARAQALELMDDYGFDAGLTRDVAMQIPADTELHRG
RGLMLGLLSKRLPVAPVDPLERGGVIALVGPTGAGKTTTIAKLAQRFAAQHTPRDVALVTTDTQRVGGREQLHSYGRQLG
VAVHEADSAESLLDLFERLRDYKLVLIDTAGMGQRDRALAAQLNWLRAAQQVTSLLVLPANAHFSDLDEVVRRFAHAKPQ
GVVLTKLDETGRFGSALSVVVDHQMPITWVTDGQRVPDDLHRANAASLVLRLEDLRRAADKPCTPEHNHAVA

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1788963, Length=201, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789874, Length=215, Percent_Identity=30.6976744186047, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 59345; Mature: 59345

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRFVAPDMRTAFRMVREEHGPDAVILSNRRTAEGIEIVAASNYDEELVQRALETARS
CCCCHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
ETSGRAHNAVGPNQQAPAPQAPTKPAAPVHAPLKLAANANVSQRQRVANAAEDMIAAMAL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHH
RQPVNVPRQAPAAAPVRTASIPSPAAQALAHAVAVTTAPRQEHALSAVPEQLFADFLTTA
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
PMQAPVQAPVHAAPVQARTPIMAAALATHASYGQDDDEQDDDGFDMDDALPPIVVAPAAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEHHHHH
AVAAAAPAPQNDEELKQLRGELALMRQMIEREMNRLTDERLRGSPARAQALELMDDYGFD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
AGLTRDVAMQIPADTELHRGRGLMLGLLSKRLPVAPVDPLERGGVIALVGPTGAGKTTTI
CCCCCHHEEECCCCCHHHCCCCHHHHHHHCCCCCCCCCCHHCCCEEEEECCCCCCCHHHH
AKLAQRFAAQHTPRDVALVTTDTQRVGGREQLHSYGRQLGVAVHEADSAESLLDLFERLR
HHHHHHHHHHCCCCCEEEEEECHHHCCCHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHC
DYKLVLIDTAGMGQRDRALAAQLNWLRAAQQVTSLLVLPANAHFSDLDEVVRRFAHAKPQ
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCC
GVVLTKLDETGRFGSALSVVVDHQMPITWVTDGQRVPDDLHRANAASLVLRLEDLRRAAD
CEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
KPCTPEHNHAVA
CCCCCCCCCCCC
>Mature Secondary Structure
MKIKRFVAPDMRTAFRMVREEHGPDAVILSNRRTAEGIEIVAASNYDEELVQRALETARS
CCCCHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
ETSGRAHNAVGPNQQAPAPQAPTKPAAPVHAPLKLAANANVSQRQRVANAAEDMIAAMAL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHH
RQPVNVPRQAPAAAPVRTASIPSPAAQALAHAVAVTTAPRQEHALSAVPEQLFADFLTTA
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
PMQAPVQAPVHAAPVQARTPIMAAALATHASYGQDDDEQDDDGFDMDDALPPIVVAPAAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEHHHHH
AVAAAAPAPQNDEELKQLRGELALMRQMIEREMNRLTDERLRGSPARAQALELMDDYGFD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
AGLTRDVAMQIPADTELHRGRGLMLGLLSKRLPVAPVDPLERGGVIALVGPTGAGKTTTI
CCCCCHHEEECCCCCHHHCCCCHHHHHHHCCCCCCCCCCHHCCCEEEEECCCCCCCHHHH
AKLAQRFAAQHTPRDVALVTTDTQRVGGREQLHSYGRQLGVAVHEADSAESLLDLFERLR
HHHHHHHHHHCCCCCEEEEEECHHHCCCHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHC
DYKLVLIDTAGMGQRDRALAAQLNWLRAAQQVTSLLVLPANAHFSDLDEVVRRFAHAKPQ
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCC
GVVLTKLDETGRFGSALSVVVDHQMPITWVTDGQRVPDDLHRANAASLVLRLEDLRRAAD
CEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
KPCTPEHNHAVA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9503621 [H]