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The map label for this gene is rnt [H]

Identifier: 84623997

GI number: 84623997

Start: 2600795

End: 2601430

Strand: Direct

Name: rnt [H]

Synonym: XOO_2340

Alternate gene names: 84623997

Gene position: 2600795-2601430 (Clockwise)

Preceding gene: 84623996

Following gene: 84623998

Centisome position: 52.65

GC content: 63.36

Gene sequence:

>636_bases
ATGAACGAACCGGTCGATGCACAGCCTGCCCCCTATTTTTTGCCGATGTCGCGCCGCTTTCGCGGCTATCTGCCCGTGGT
GGTGGACGTCGAAACGGGCGGGTTCGATTCGAATAAGCATGCGCTGCTGGAAATCGCCTGCGTGCCGATCGAAATGGATG
CCGACGGTCGCTTCTTTCCCGGCGACACCGCCAGCGCGCATCTGGTGCCTGCGCCCGGGCTGGAGATCGACCCGAAATCG
CTGGAAATCACCGGCATCGTGCTCGACCACCCGTTTCGCTTGGCGAAACAGGAAAAGGACGCACTGGACCATGTGTTCGC
GCCGGTACGCGCGGCAGTGAAGAAATACGGCTGCCAGCGTGCGATCCTGGTTGGCCACAACGCGCATTTCGATCTGAACT
TTCTCAACGCGGCGGTGGCGCGCGTGGGCCACAAACGCAACCCGTTTCATCCCTTCAGCGTGTTCGATACGGTGACACTG
GCCGGCGTGGCTTACGGCCAGACCGTGCTGGCACGAGCCGCCCAGGCCGCGGGCCTGGACTGGAATGCCGCCGACGCGCA
CAGCGCGGTCTACGACACTGAACAGACCGCACGCCTGTTCTGCAAGATCGCCAACGCCTGGCCGGGGCCGGTTTAG

Upstream 100 bases:

>100_bases
ACGGGATGACGGCCCCGGCGATGCATGGCGCTTCCACCCGGAACACCTGCAGCCTTGCGTTCATGGCTGAACCCGGGGCG
CGACCTGCTAGGCTATCCGG

Downstream 100 bases:

>100_bases
TTCCACTGTGTTACAAATAATCGTATCTTTGTGCCACTATTGGAAGACCTTCAGGCCGCGTGGGAGAGCTGTGTTGAATA
GGTCACTGGAAGTGCGTTTT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNEPVDAQPAPYFLPMSRRFRGYLPVVVDVETGGFDSNKHALLEIACVPIEMDADGRFFPGDTASAHLVPAPGLEIDPKS
LEITGIVLDHPFRLAKQEKDALDHVFAPVRAAVKKYGCQRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTL
AGVAYGQTVLARAAQAAGLDWNAADAHSAVYDTEQTARLFCKIANAWPGPV

Sequences:

>Translated_211_residues
MNEPVDAQPAPYFLPMSRRFRGYLPVVVDVETGGFDSNKHALLEIACVPIEMDADGRFFPGDTASAHLVPAPGLEIDPKS
LEITGIVLDHPFRLAKQEKDALDHVFAPVRAAVKKYGCQRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTL
AGVAYGQTVLARAAQAAGLDWNAADAHSAVYDTEQTARLFCKIANAWPGPV
>Mature_211_residues
MNEPVDAQPAPYFLPMSRRFRGYLPVVVDVETGGFDSNKHALLEIACVPIEMDADGRFFPGDTASAHLVPAPGLEIDPKS
LEITGIVLDHPFRLAKQEKDALDHVFAPVRAAVKKYGCQRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTL
AGVAYGQTVLARAAQAAGLDWNAADAHSAVYDTEQTARLFCKIANAWPGPV

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=192, Percent_Identity=50.5208333333333, Blast_Score=201, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.- [C]

Molecular weight: Translated: 22898; Mature: 22898

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPVDAQPAPYFLPMSRRFRGYLPVVVDVETGGFDSNKHALLEIACVPIEMDADGRFFP
CCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCC
GDTASAHLVPAPGLEIDPKSLEITGIVLDHPFRLAKQEKDALDHVFAPVRAAVKKYGCQR
CCCCCEEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTLAGVAYGQTVLARAAQAAGLD
EEEEECCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WNAADAHSAVYDTEQTARLFCKIANAWPGPV
CCCCCCCHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNEPVDAQPAPYFLPMSRRFRGYLPVVVDVETGGFDSNKHALLEIACVPIEMDADGRFFP
CCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCC
GDTASAHLVPAPGLEIDPKSLEITGIVLDHPFRLAKQEKDALDHVFAPVRAAVKKYGCQR
CCCCCEEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTLAGVAYGQTVLARAAQAAGLD
EEEEECCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WNAADAHSAVYDTEQTARLFCKIANAWPGPV
CCCCCCCHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA