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The map label for this gene is ispG [H]

Identifier: 84623752

GI number: 84623752

Start: 2318026

End: 2319291

Strand: Direct

Name: ispG [H]

Synonym: XOO_2095

Alternate gene names: 84623752

Gene position: 2318026-2319291 (Clockwise)

Preceding gene: 84623749

Following gene: 84623753

Centisome position: 46.92

GC content: 65.17

Gene sequence:

>1266_bases
ATGCATGACGCCGTGACCCGCCCAACCCCTCCCGCCGACGCCACCGCCTGGCCGCGCCGCATCACTCAGGCCGTCAAGAT
CGGCGGCGTGACCGTGGGCGGAGGCCATCCGGTCGTCGTCCAGTCGATGACCAACACCGACACTGCCGACATTGCCGGCA
GCGTCAAGCAGGTGGCCGATCTGTGGCGCGCCGGCTCGGAAATGGTGCGCCTGACCGTCAACAACGCCGAGTCCGCTGCC
GCCATCCCGCGCATCGTCGACAAGCTGCGCATGATGGGCATCGACGTACCGTTGATCGGCGACTTCCATTACAACGGCCA
CCAGTTGCTGGCGGCAGAGCCGGCCTGCGCCGAGGCGCTGGCCAAGTACCGCATCAATCCCGGCAATGTCGGTTTCGGCA
AGAAGAAGGATCTGCAGTTCGGTCAGTTGATCGAATGTGCGATCAAGTACGACAAACCGGTGCGCATCGGCGCTAACTGG
GGCTCGCTGGACCAGTCGCTGGCCGCGCAGCTGATGGACGAAAACGCGCAGCGCGAAACGCCCTGGGATGCCGGCCGGGT
GCTGCGCGAGGCACTGATCCGCTCGGCCTTGGATTCGGCCGAGCGTGCGGTGGAGCTGGGCCTGCCGCGTGAGCGCATCA
TTCTGTCGGCCAAGGTGTCCGGCGTGCAGGAGTTGATTGCGGTGTATCGCGACATGGCCAGCCGCTGCGATTTCGCGTTG
CATCTGGGCCTGACCGAAGCCGGGATCGGCAGCAAGGGCATCGTGGCCTCGGCGGCGGCACTGAGCGTGCTGTTGCAGGA
AGGCATCGGCGACACCATCCGTATCTCGCTGACCCCAGAGCCGGGGCAGTCGCGCACTCACGAAGTGATCGTCGCGCAGG
AACTGCTGCAGACCACCGGCCAGCGTGCGTTTACGCCGATGGTCACCGCCTGTCCGGGCTGCGGCCGCACCACCTCCGAG
TTCTTCCAGGAGCTGGCCGGGGTGGTGCAGAACCATGTGCGCGCCAAGATGCCGGAATGGAAGATCACCAATCCCGGCGC
CGAGAACATGACGCTGGCGGTGATGGGCTGCGTGGTCAACGGACCAGGCGAATCGCGCCACGCCAATATCGGTATTTCGT
TGCCGGGTACCGGCGAGGCGCCATCCGCGCCAGTGTTCATCGATGGCGAAAAAAGCGTCATCCTGCGCGGCGAAAACATC
GCTCAAGAGTTCATTGGCCTGATCGATCAATACGTCGAACGCACCTATGCCCGCCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
GGGGAGGTGGTGGCCTGCGACAAACCGTCGCATCGGCCCGAATGGCGGCCCGCACGGCGCTTCAGCTGTGAGCGCACGAC
GGACAGGCTAAAATACTGCG

Downstream 100 bases:

>100_bases
ATCGCCGGCCGGTGTCAAGGGATGGTTGCGTAGCACTGCGTGGCGGACGGCGCTGTTGTTCGCCGGGGTGCTGCTACCGC
TGGGTTTATTCGTAGACCTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MHDAVTRPTPPADATAWPRRITQAVKIGGVTVGGGHPVVVQSMTNTDTADIAGSVKQVADLWRAGSEMVRLTVNNAESAA
AIPRIVDKLRMMGIDVPLIGDFHYNGHQLLAAEPACAEALAKYRINPGNVGFGKKKDLQFGQLIECAIKYDKPVRIGANW
GSLDQSLAAQLMDENAQRETPWDAGRVLREALIRSALDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTHEVIVAQELLQTTGQRAFTPMVTACPGCGRTTSE
FFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPSAPVFIDGEKSVILRGENI
AQEFIGLIDQYVERTYARRAG

Sequences:

>Translated_421_residues
MHDAVTRPTPPADATAWPRRITQAVKIGGVTVGGGHPVVVQSMTNTDTADIAGSVKQVADLWRAGSEMVRLTVNNAESAA
AIPRIVDKLRMMGIDVPLIGDFHYNGHQLLAAEPACAEALAKYRINPGNVGFGKKKDLQFGQLIECAIKYDKPVRIGANW
GSLDQSLAAQLMDENAQRETPWDAGRVLREALIRSALDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTHEVIVAQELLQTTGQRAFTPMVTACPGCGRTTSE
FFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPSAPVFIDGEKSVILRGENI
AQEFIGLIDQYVERTYARRAG
>Mature_421_residues
MHDAVTRPTPPADATAWPRRITQAVKIGGVTVGGGHPVVVQSMTNTDTADIAGSVKQVADLWRAGSEMVRLTVNNAESAA
AIPRIVDKLRMMGIDVPLIGDFHYNGHQLLAAEPACAEALAKYRINPGNVGFGKKKDLQFGQLIECAIKYDKPVRIGANW
GSLDQSLAAQLMDENAQRETPWDAGRVLREALIRSALDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTHEVIVAQELLQTTGQRAFTPMVTACPGCGRTTSE
FFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPSAPVFIDGEKSVILRGENI
AQEFIGLIDQYVERTYARRAG

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=400, Percent_Identity=32.75, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45027; Mature: 45027

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDAVTRPTPPADATAWPRRITQAVKIGGVTVGGGHPVVVQSMTNTDTADIAGSVKQVAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LWRAGSEMVRLTVNNAESAAAIPRIVDKLRMMGIDVPLIGDFHYNGHQLLAAEPACAEAL
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECEECCCCEEEECCCHHHHHH
AKYRINPGNVGFGKKKDLQFGQLIECAIKYDKPVRIGANWGSLDQSLAAQLMDENAQRET
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCC
PWDAGRVLREALIRSALDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCEEE
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTHEVIVAQELLQTTG
EECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCC
QRAFTPMVTACPGCGRTTSEFFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVN
CHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEEEEC
GPGESRHANIGISLPGTGEAPSAPVFIDGEKSVILRGENIAQEFIGLIDQYVERTYARRA
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MHDAVTRPTPPADATAWPRRITQAVKIGGVTVGGGHPVVVQSMTNTDTADIAGSVKQVAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LWRAGSEMVRLTVNNAESAAAIPRIVDKLRMMGIDVPLIGDFHYNGHQLLAAEPACAEAL
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECEECCCCEEEECCCHHHHHH
AKYRINPGNVGFGKKKDLQFGQLIECAIKYDKPVRIGANWGSLDQSLAAQLMDENAQRET
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCC
PWDAGRVLREALIRSALDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCEEE
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTHEVIVAQELLQTTG
EECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCC
QRAFTPMVTACPGCGRTTSEFFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVN
CHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEEEEC
GPGESRHANIGISLPGTGEAPSAPVFIDGEKSVILRGENIAQEFIGLIDQYVERTYARRA
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]