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The map label for this gene is purR [H]

Identifier: 84623607

GI number: 84623607

Start: 2151356

End: 2152525

Strand: Direct

Name: purR [H]

Synonym: XOO_1950

Alternate gene names: 84623607

Gene position: 2151356-2152525 (Clockwise)

Preceding gene: 84623606

Following gene: 84623608

Centisome position: 43.55

GC content: 64.53

Gene sequence:

>1170_bases
ATGTATGCGCCTTTCTTTCACCGCAAGGCTTGCCACGTGACACCCGCCAAGCCGACACCCGAGTGCACTGCGGGTGGTGA
CGCGCGACGCGCGCGTCGCGGCGCGGTGACCTTGCGCGATATCGCAGGGGCCATTGGTGTGTCGCGGGCGACGGTGTCGC
TGGTGCTGCGCGGTAGCCCGCTGGTGCATGCAAGCACGCGCGCGTGCGTGGAGGCCGAGCTGGATCGGCAGAATTATGTC
TACAACCGTACTGCGGCGAATTTGCGCCAGCGCACCTCGTCCAGCATCGCGCTGGTCATCAACGATTTGTCCAACCCCTT
TTTTGCCGAATTCGCCGCCGGTGTCAACGAAGCATTGGGCGCGGCCGGTTATGTGACCCTGCTCGGCAGCACCGGCGAAT
CGACGCAGCGTCAACAGGTGGTGCTGACCTCGTTGATGGAGCACACGCCGGCGGGCATCATCCTGTCGCCGGCCGAAGGC
AGCCAGGCGCAGGCGCTCAGCCAGGTACTCGGCCGCCAACGTAATGTGGTGCTGTTCAACCGTGAGGTAGAAGGCGCGCA
GTGGGATCTGCTGGCGTTGGACAATGCGCAGGGCGCGTTTCTGGCGTGCACCCATCTGATCGCGCAAGGGCATCGGCGCA
TCGTGTTTTTTGGTGGGCATGCTGCATCCAGTTCATGTCGCCAGCGTCGCGCAGGCTATGCGCGCGCGATGCAGGAAGCA
GGCTTGGACACCCTGTACTTCGAGGCGGCACCCAATCGCCAGGAAGCTGCACTATGTGGCGTGCGCTTGCTCAGCAGCGA
TGCGCAAGCTGCGACTGCGGCAGTCTGCTACAACGACAGCGTTGCGCTGGGCTTGATGGCGGCGTTGGCGAAGCACGGCA
TCCTGCCAGGCCGCGACTTTGCGATGACCGGCTTCGACGACATCGCCGAAGCAGCGTTGTTCACGCCTGCATTGACAACC
CTGGCCGCACACCCGCGTGCGCGCGGAAGACAGGCCGCGCAACTGGTATTGCAGCGGATCGGTAGCCCCGAGCTCGCCGC
GCAGCAATTGACCGCAGCAGTCGCCCTGAAAATTCGCGCAAGCAGCGCCTGCGCGCCCGTAGCTCGCATGTCATCACCTC
CCCCTAGCAGTCCATTCGCCCATTCCCCCACCAAGGCCTCAGGCCGCTGA

Upstream 100 bases:

>100_bases
GTCGCGGATTTCTGTGCGGGCGCTGGATTGTCCATGTTGCAGTGCCGCATGCATTGGCAGCCCGGAAAAGGCCTAATTGG
ATCGATCCAATTTGATGCCA

Downstream 100 bases:

>100_bases
CCAGGCAATCCCTATGGTTTCCCTTTCCACGCAATCCTCCGTGCCCAGTGGCAACAAGGCGGCCGTCAACAACGGCGTCG
CGTTGTCGGTGGTGACCACG

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MYAPFFHRKACHVTPAKPTPECTAGGDARRARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRACVEAELDRQNYV
YNRTAANLRQRTSSSIALVINDLSNPFFAEFAAGVNEALGAAGYVTLLGSTGESTQRQQVVLTSLMEHTPAGIILSPAEG
SQAQALSQVLGRQRNVVLFNREVEGAQWDLLALDNAQGAFLACTHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEA
GLDTLYFEAAPNRQEAALCGVRLLSSDAQAATAAVCYNDSVALGLMAALAKHGILPGRDFAMTGFDDIAEAALFTPALTT
LAAHPRARGRQAAQLVLQRIGSPELAAQQLTAAVALKIRASSACAPVARMSSPPPSSPFAHSPTKASGR

Sequences:

>Translated_389_residues
MYAPFFHRKACHVTPAKPTPECTAGGDARRARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRACVEAELDRQNYV
YNRTAANLRQRTSSSIALVINDLSNPFFAEFAAGVNEALGAAGYVTLLGSTGESTQRQQVVLTSLMEHTPAGIILSPAEG
SQAQALSQVLGRQRNVVLFNREVEGAQWDLLALDNAQGAFLACTHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEA
GLDTLYFEAAPNRQEAALCGVRLLSSDAQAATAAVCYNDSVALGLMAALAKHGILPGRDFAMTGFDDIAEAALFTPALTT
LAAHPRARGRQAAQLVLQRIGSPELAAQQLTAAVALKIRASSACAPVARMSSPPPSSPFAHSPTKASGR
>Mature_389_residues
MYAPFFHRKACHVTPAKPTPECTAGGDARRARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRACVEAELDRQNYV
YNRTAANLRQRTSSSIALVINDLSNPFFAEFAAGVNEALGAAGYVTLLGSTGESTQRQQVVLTSLMEHTPAGIILSPAEG
SQAQALSQVLGRQRNVVLFNREVEGAQWDLLALDNAQGAFLACTHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEA
GLDTLYFEAAPNRQEAALCGVRLLSSDAQAATAAVCYNDSVALGLMAALAKHGILPGRDFAMTGFDDIAEAALFTPALTT
LAAHPRARGRQAAQLVLQRIGSPELAAQQLTAAVALKIRASSACAPVARMSSPPPSSPFAHSPTKASGR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=32.1799307958478, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=31.1926605504587, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=26.9005847953216, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=29.6969696969697, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=29.7385620915033, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=26.2686567164179, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=26.1538461538462, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786540, Length=343, Percent_Identity=26.2390670553936, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789068, Length=289, Percent_Identity=26.2975778546713, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=25.0783699059561, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40967; Mature: 40967

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAPFFHRKACHVTPAKPTPECTAGGDARRARRGAVTLRDIAGAIGVSRATVSLVLRGSP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCC
LVHASTRACVEAELDRQNYVYNRTAANLRQRTSSSIALVINDLSNPFFAEFAAGVNEALG
CEECCHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHC
AAGYVTLLGSTGESTQRQQVVLTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFN
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEE
REVEGAQWDLLALDNAQGAFLACTHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEA
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GLDTLYFEAAPNRQEAALCGVRLLSSDAQAATAAVCYNDSVALGLMAALAKHGILPGRDF
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHHHHCCCCCCCCE
AMTGFDDIAEAALFTPALTTLAAHPRARGRQAAQLVLQRIGSPELAAQQLTAAVALKIRA
EECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEC
SSACAPVARMSSPPPSSPFAHSPTKASGR
CCCCCHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYAPFFHRKACHVTPAKPTPECTAGGDARRARRGAVTLRDIAGAIGVSRATVSLVLRGSP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCC
LVHASTRACVEAELDRQNYVYNRTAANLRQRTSSSIALVINDLSNPFFAEFAAGVNEALG
CEECCHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHC
AAGYVTLLGSTGESTQRQQVVLTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFN
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEE
REVEGAQWDLLALDNAQGAFLACTHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEA
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GLDTLYFEAAPNRQEAALCGVRLLSSDAQAATAAVCYNDSVALGLMAALAKHGILPGRDF
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHHHHCCCCCCCCE
AMTGFDDIAEAALFTPALTTLAAHPRARGRQAAQLVLQRIGSPELAAQQLTAAVALKIRA
EECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEC
SSACAPVARMSSPPPSSPFAHSPTKASGR
CCCCCHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA