Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 84623546

GI number: 84623546

Start: 2086013

End: 2087530

Strand: Direct

Name: oprM [H]

Synonym: XOO_1889

Alternate gene names: 84623546

Gene position: 2086013-2087530 (Clockwise)

Preceding gene: 84623545

Following gene: 84623547

Centisome position: 42.23

GC content: 68.58

Gene sequence:

>1518_bases
ATGAACGCTGCTAGTTCCCCGACCCAGACGTTGCGCCCGCGCTCGATGCGCTTGGCGCGGCCGTTCGTTATCACTGCCCT
TGCGCTGGCGCTGGCCGCCTGTGCCAGCAGCCGTGGCCTAACCCCGCAGGGTAGCGTGCTGGATCCCAGCAGGCTGCATG
CGGAGCGCACCCTGGCGCAGCAAACCACGCTGAGCCCGGCCGCGTGGCCGGCCAGCGATTGGTGGCGCGCGCTGGGCGAT
GCGCAGCTGGACGCGCTGATTGCCGAGGGCCTGCAGCACAGCCCCAGCCTTGTGGCGGCCGACGCGCGCTTGCATCGGGT
GCAGGCCCGTATCGACACTGCGCAGGCCAATCGTGGCCCCAGCTTGTCGGTGTCCGGTGGCTACACCGGCCTGCAGCTGC
CCGAATCCTTGGTCGGCGAAGAGATCGGTGGCAAATACGGCGGTAGTGCGCAGCTCGCGTTGAAGTTCCGCTACGGCGTC
GATCTGTGGGGCGGCAAGCGCAGCGCGTGGGAAGCGGCGGTCGATCAGGCGCATGCAGCCGAAGTGGATGCGCAGGCGGC
GCGGTTGAATCTGTCGTCGGCGATTGCCGAAGGCTATGCGCAGCTTGCTTACGCATGGAGTTTGCATGACCTGGCCAATG
ACGAATTAGGCCGCGCCCAGAAGCTGCTGGAATTGACCCGGCAACGCCGCAGCGCCGGCATCGACAGCGAGTTGCAGGTG
CGTCAGGCGCAGGCGCGCGTGCCGGCTGCGCAGCAGCAGCTGCAGTCCGCGCAGCAGCAGATCGACGAAGCCCGCACTGC
GCTGGCGGCGTTGGTCGGGCAGGGCCCGGATCGCGGCTTGGACATCGCCCGCCCGGTGCTGGGCGGGGGCATTGCCACCC
AGTTGCCGAGCATGCTGCCGGCCGATCTGCTCGGCCGTCGTCCCGACGTGGTCGCCGCGCGCTGGCGCGTGGAGGCGGCC
GACAAGGACATCGCGGTCGCCAAGACCCGCTTCTATCCCAGCCTCAACCTCACTGCGCTGGGCGGTGTGATCAACCCGGA
TGTCGGCAAGTTGCTGGACAGTGGCTCGGTGTTCGGTCTGGTCGCGCCGGCGCTGAGCCTGCCGATCTTCGACGGCGGCA
AGCTGCGCGCCAACCTGGCCGGCAGCGATGCGCAATACGACCTGGCCGTGGCCGACTACAACCAGAAAGTGATCTCTGCG
CTGCGCGAAGTGGCCGACCAGGTCACCGCCGTGCGCTCGCTGCAGCAACGCGCGCAGGCGCAGAACGACGCCGTGCAGAC
CGCCATGGCGGCCTTCGATCTGGCCCAGCAGCGCTACCGCGCCGGCATCGGCAGCTATCTGGAAGTGCTTCGCGTGCAGG
AGCAGGTGCTCGTCGCACGCCAACGCATGGCTGGGCTGCAGTCTCAACAACTTCTGGCCTCGGTGAGGTTGCAGCGTGCG
CTGGGCGGCGGATTCACGCCGGAGCCCGCGCGCGATACCGCACACACCGCGCCCACTTCCGCACAACCGAATTCCTGA

Upstream 100 bases:

>100_bases
GCGGCGAACGCGCCATCGAGCAAGGCCTGCGCGATCTGTCTGCGCCCGAGCGCGAACTTCTTACCTCACTTCTCATCCGT
GTCCGCGATGCACTCAACGC

Downstream 100 bases:

>100_bases
GACATCGACGATGAGCCAGACGACTTCAACTTCTCCTCAACCCGCACCAGCCGCGCCCAGCAAGCGCCGCGCTGCGCTGC
GTATCGTGGCCATTGTGGCG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNAASSPTQTLRPRSMRLARPFVITALALALAACASSRGLTPQGSVLDPSRLHAERTLAQQTTLSPAAWPASDWWRALGD
AQLDALIAEGLQHSPSLVAADARLHRVQARIDTAQANRGPSLSVSGGYTGLQLPESLVGEEIGGKYGGSAQLALKFRYGV
DLWGGKRSAWEAAVDQAHAAEVDAQAARLNLSSAIAEGYAQLAYAWSLHDLANDELGRAQKLLELTRQRRSAGIDSELQV
RQAQARVPAAQQQLQSAQQQIDEARTALAALVGQGPDRGLDIARPVLGGGIATQLPSMLPADLLGRRPDVVAARWRVEAA
DKDIAVAKTRFYPSLNLTALGGVINPDVGKLLDSGSVFGLVAPALSLPIFDGGKLRANLAGSDAQYDLAVADYNQKVISA
LREVADQVTAVRSLQQRAQAQNDAVQTAMAAFDLAQQRYRAGIGSYLEVLRVQEQVLVARQRMAGLQSQQLLASVRLQRA
LGGGFTPEPARDTAHTAPTSAQPNS

Sequences:

>Translated_505_residues
MNAASSPTQTLRPRSMRLARPFVITALALALAACASSRGLTPQGSVLDPSRLHAERTLAQQTTLSPAAWPASDWWRALGD
AQLDALIAEGLQHSPSLVAADARLHRVQARIDTAQANRGPSLSVSGGYTGLQLPESLVGEEIGGKYGGSAQLALKFRYGV
DLWGGKRSAWEAAVDQAHAAEVDAQAARLNLSSAIAEGYAQLAYAWSLHDLANDELGRAQKLLELTRQRRSAGIDSELQV
RQAQARVPAAQQQLQSAQQQIDEARTALAALVGQGPDRGLDIARPVLGGGIATQLPSMLPADLLGRRPDVVAARWRVEAA
DKDIAVAKTRFYPSLNLTALGGVINPDVGKLLDSGSVFGLVAPALSLPIFDGGKLRANLAGSDAQYDLAVADYNQKVISA
LREVADQVTAVRSLQQRAQAQNDAVQTAMAAFDLAQQRYRAGIGSYLEVLRVQEQVLVARQRMAGLQSQQLLASVRLQRA
LGGGFTPEPARDTAHTAPTSAQPNS
>Mature_505_residues
MNAASSPTQTLRPRSMRLARPFVITALALALAACASSRGLTPQGSVLDPSRLHAERTLAQQTTLSPAAWPASDWWRALGD
AQLDALIAEGLQHSPSLVAADARLHRVQARIDTAQANRGPSLSVSGGYTGLQLPESLVGEEIGGKYGGSAQLALKFRYGV
DLWGGKRSAWEAAVDQAHAAEVDAQAARLNLSSAIAEGYAQLAYAWSLHDLANDELGRAQKLLELTRQRRSAGIDSELQV
RQAQARVPAAQQQLQSAQQQIDEARTALAALVGQGPDRGLDIARPVLGGGIATQLPSMLPADLLGRRPDVVAARWRVEAA
DKDIAVAKTRFYPSLNLTALGGVINPDVGKLLDSGSVFGLVAPALSLPIFDGGKLRANLAGSDAQYDLAVADYNQKVISA
LREVADQVTAVRSLQQRAQAQNDAVQTAMAAFDLAQQRYRAGIGSYLEVLRVQEQVLVARQRMAGLQSQQLLASVRLQRA
LGGGFTPEPARDTAHTAPTSAQPNS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI2367348, Length=470, Percent_Identity=29.7872340425532, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786785, Length=480, Percent_Identity=25.4166666666667, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53586; Mature: 53586

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAASSPTQTLRPRSMRLARPFVITALALALAACASSRGLTPQGSVLDPSRLHAERTLAQ
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
QTTLSPAAWPASDWWRALGDAQLDALIAEGLQHSPSLVAADARLHRVQARIDTAQANRGP
HHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
SLSVSGGYTGLQLPESLVGEEIGGKYGGSAQLALKFRYGVDLWGGKRSAWEAAVDQAHAA
CEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECEEECCCCHHHHHHHHHHHHHH
EVDAQAARLNLSSAIAEGYAQLAYAWSLHDLANDELGRAQKLLELTRQRRSAGIDSELQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
RQAQARVPAAQQQLQSAQQQIDEARTALAALVGQGPDRGLDIARPVLGGGIATQLPSMLP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCC
ADLLGRRPDVVAARWRVEAADKDIAVAKTRFYPSLNLTALGGVINPDVGKLLDSGSVFGL
HHHHCCCCCCEEHHEEEECCCCCHHHHHHHCCCCCCHHEECCCCCCCHHHHHCCCCHHHH
VAPALSLPIFDGGKLRANLAGSDAQYDLAVADYNQKVISALREVADQVTAVRSLQQRAQA
HHHHHHCCCCCCCCEEEEECCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QNDAVQTAMAAFDLAQQRYRAGIGSYLEVLRVQEQVLVARQRMAGLQSQQLLASVRLQRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LGGGFTPEPARDTAHTAPTSAQPNS
HCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAASSPTQTLRPRSMRLARPFVITALALALAACASSRGLTPQGSVLDPSRLHAERTLAQ
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
QTTLSPAAWPASDWWRALGDAQLDALIAEGLQHSPSLVAADARLHRVQARIDTAQANRGP
HHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
SLSVSGGYTGLQLPESLVGEEIGGKYGGSAQLALKFRYGVDLWGGKRSAWEAAVDQAHAA
CEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECEEECCCCHHHHHHHHHHHHHH
EVDAQAARLNLSSAIAEGYAQLAYAWSLHDLANDELGRAQKLLELTRQRRSAGIDSELQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
RQAQARVPAAQQQLQSAQQQIDEARTALAALVGQGPDRGLDIARPVLGGGIATQLPSMLP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCC
ADLLGRRPDVVAARWRVEAADKDIAVAKTRFYPSLNLTALGGVINPDVGKLLDSGSVFGL
HHHHCCCCCCEEHHEEEECCCCCHHHHHHHCCCCCCHHEECCCCCCCHHHHHCCCCHHHH
VAPALSLPIFDGGKLRANLAGSDAQYDLAVADYNQKVISALREVADQVTAVRSLQQRAQA
HHHHHHCCCCCCCCEEEEECCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QNDAVQTAMAAFDLAQQRYRAGIGSYLEVLRVQEQVLVARQRMAGLQSQQLLASVRLQRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LGGGFTPEPARDTAHTAPTSAQPNS
HCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]