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The map label for this gene is pilJ [H]

Identifier: 84623307

GI number: 84623307

Start: 1798371

End: 1800407

Strand: Direct

Name: pilJ [H]

Synonym: XOO_1650

Alternate gene names: 84623307

Gene position: 1798371-1800407 (Clockwise)

Preceding gene: 84623306

Following gene: 84623308

Centisome position: 36.4

GC content: 64.65

Gene sequence:

>2037_bases
ATGAGTACCGCCCCGGACGCCCGCAAGACCAACAAGCTCGGCAGCGTCAGCACCAGCTTCTGGCTTGGCTTGCTGGTGTT
GTCGATGATCGTGTTTGGCGCCAACACCGGCGTCGCGACCTGGCAGAGCAGTCATCTTGCCGGCGCCGGTGTTAGCGCGG
CCGACTTGCAGGTGCTTTCGCAGCAGCTGGCTAACCAGGGCCGCGAAGCGGTCTCCGGCGACACTAAGGCATTTGCCGCG
TTCAAGGAGACCAAGGGCCGCATCGACAGCACGGTCAACGATCTGGATGGCCGTTACGGCCAGGAAGGCCCGATCGCCAG
CTCGATGTCGCAGCTCAAGGCGACCTGGGTGCCGCTGAGCAAGAACGCCGATCAGGTCATCGCCAGCGAACAGGCGGTGC
TGGCGATCGCAGTCAACGCCAACCGCTTCTCCGGCAGCGTCCCGCAATTGCAGGCGCAGTTGAACGAAGTGGTGCGTGCG
ATGACCGTCAGCGGCGCGCCGGCTTCGCAGATCTACAACACCTTGCAGCAGGTCGTGGTAGCCGGAACCATGGCGCGGCG
GGTGACCGAAATCCGCGCCGGCGAAGACGATGCGGTCGCGTCCGGCGACGCGCTGGCACGCGACTCGGTGGTGTTCACGC
AGATGCTCGAAGGCTTGCGCGCAGGCAACGAGGAACTCGGCATCGCCGCGGTCCGAAACCCGGCCGCGTTGTCGGCGCTG
GAACAGTCGCAGGCCCAGTGGACGAGGATGAAGAGGGACGTCGACGCCATCCTCGCCAACTCGCGCAACCTGTTTGCCGC
GCAGTCCTCGGCTGCCGCGCTGACGGCTGGCTCCAGCAAGATGCTCGACGACAGCAAGAAGCTGTTCGATGCGTTCTCTT
CGTTCGGTTCGGTGCGCGACACGCGCCTGTTCCCGAACTTCTGGCTGGGCGTGGTGTCCGGCCTGGTCGCGCTGCTGGCG
ATCATCGGCTTCGTGTGGAGCTCGGTGCGCGTGCGGACCCGCGAGCAGGACGTGCGCTACCAGTCGCAGGTGGAATTCAA
CAGCCGCAACCAGCAGGCGATTATGCGGTTGCTGGACGAAATCAGCTCGCTCGGTGAGGGTGACCTGACGGTGAAAGCCT
CGGTGACCGAGGACATGACCGGTGCCATCGCCGATGCCATCAACTACGCCGTGGACGAACTGCGCCACCTGGTGACCACC
ATCAACGACACCTCAGCCAAAGTGGCCGTGTCGACCCAGGAAACCCAGGCCACGGCCATGCAGCTGGCCGAAGCGGCCGG
CCAGCAGGCCAACCAGATCACCACTGCTTCCGAGCGCATCAGCGAAATCGCTGCCAGCATCGAACAGGTGTCGCGCAACT
CCACCGAGTCGGCCGAAGTGGCGCAGCGCTCGGTGGTCATCGCCGCCGAAGGTGCCGGCGTGGTGCGCGAGACGATTCAG
GGCATGGATCAGATTCGCGACCAGATCCAGGAAACCTCCAAGCGCATCAAGCGGCTGGGCGAATCGTCGCAGGAAATCGG
CTCGATCGTGGAACTGATCAACGACATTTCCGAGCAGACCAACATCCTGGCGCTCAACGCTGCGGTGCAGGCAGCATCGG
CCGGCGAGGCCGGCCGCGGTTTCGCCGTGGTGGCCGACGAAGTGCAGCGCCTGGCAGAACGCACCTCCAGTGCGACCCGA
CGCATTGAAGGTCTGGTGCAGACCATTCAGGCCGATACCAACGAAGCGGTCAGCTCGATGGAGCAGACCACCTCCGAAGT
GGTGTCCGGTGCGCGCCTGGCCGAAGACGCCGGCACCGCGCTGACCGAAATCGAGCGCGTGTCCAACGCCTTGAATAACC
TGATTAAGAACATCTCCATCGCCGCGCACCAGCAGTCGGCCGCGGCGACGGATATCACACAGACCATGGGCGTGATCCGT
CAGATCACCAGCCAGACATCGCAGGGTGCGGAGCAGACGGCCGAATCGATCGGCAACCTGGCTCAGCTCGCTGCCGATCT
GCGCCGTTCGGTCGCCGACTTCAAGCTGCCGGCGTGA

Upstream 100 bases:

>100_bases
GGGGGTGTTTTCCCTGGCCTTGCTGTCGCGCACTCCTGAATTCCGGCAGGCCGCGGCCTAAGCCGCGACCCTGCCACGTC
ATACAGATCGAGGTCGAACC

Downstream 100 bases:

>100_bases
CAACGACGCGGAACGGGAAAGGATCAGCAGATGGCGCCTAGTAGCGTTACGTCGATGCAGAAAGTGACCGGTTGCTGCGC
TAGCGGCAAGTCGGTCGTAG

Product: pilus biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSTAPDARKTNKLGSVSTSFWLGLLVLSMIVFGANTGVATWQSSHLAGAGVSAADLQVLSQQLANQGREAVSGDTKAFAA
FKETKGRIDSTVNDLDGRYGQEGPIASSMSQLKATWVPLSKNADQVIASEQAVLAIAVNANRFSGSVPQLQAQLNEVVRA
MTVSGAPASQIYNTLQQVVVAGTMARRVTEIRAGEDDAVASGDALARDSVVFTQMLEGLRAGNEELGIAAVRNPAALSAL
EQSQAQWTRMKRDVDAILANSRNLFAAQSSAAALTAGSSKMLDDSKKLFDAFSSFGSVRDTRLFPNFWLGVVSGLVALLA
IIGFVWSSVRVRTREQDVRYQSQVEFNSRNQQAIMRLLDEISSLGEGDLTVKASVTEDMTGAIADAINYAVDELRHLVTT
INDTSAKVAVSTQETQATAMQLAEAAGQQANQITTASERISEIAASIEQVSRNSTESAEVAQRSVVIAAEGAGVVRETIQ
GMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRGFAVVADEVQRLAERTSSATR
RIEGLVQTIQADTNEAVSSMEQTTSEVVSGARLAEDAGTALTEIERVSNALNNLIKNISIAAHQQSAAATDITQTMGVIR
QITSQTSQGAEQTAESIGNLAQLAADLRRSVADFKLPA

Sequences:

>Translated_678_residues
MSTAPDARKTNKLGSVSTSFWLGLLVLSMIVFGANTGVATWQSSHLAGAGVSAADLQVLSQQLANQGREAVSGDTKAFAA
FKETKGRIDSTVNDLDGRYGQEGPIASSMSQLKATWVPLSKNADQVIASEQAVLAIAVNANRFSGSVPQLQAQLNEVVRA
MTVSGAPASQIYNTLQQVVVAGTMARRVTEIRAGEDDAVASGDALARDSVVFTQMLEGLRAGNEELGIAAVRNPAALSAL
EQSQAQWTRMKRDVDAILANSRNLFAAQSSAAALTAGSSKMLDDSKKLFDAFSSFGSVRDTRLFPNFWLGVVSGLVALLA
IIGFVWSSVRVRTREQDVRYQSQVEFNSRNQQAIMRLLDEISSLGEGDLTVKASVTEDMTGAIADAINYAVDELRHLVTT
INDTSAKVAVSTQETQATAMQLAEAAGQQANQITTASERISEIAASIEQVSRNSTESAEVAQRSVVIAAEGAGVVRETIQ
GMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRGFAVVADEVQRLAERTSSATR
RIEGLVQTIQADTNEAVSSMEQTTSEVVSGARLAEDAGTALTEIERVSNALNNLIKNISIAAHQQSAAATDITQTMGVIR
QITSQTSQGAEQTAESIGNLAQLAADLRRSVADFKLPA
>Mature_677_residues
STAPDARKTNKLGSVSTSFWLGLLVLSMIVFGANTGVATWQSSHLAGAGVSAADLQVLSQQLANQGREAVSGDTKAFAAF
KETKGRIDSTVNDLDGRYGQEGPIASSMSQLKATWVPLSKNADQVIASEQAVLAIAVNANRFSGSVPQLQAQLNEVVRAM
TVSGAPASQIYNTLQQVVVAGTMARRVTEIRAGEDDAVASGDALARDSVVFTQMLEGLRAGNEELGIAAVRNPAALSALE
QSQAQWTRMKRDVDAILANSRNLFAAQSSAAALTAGSSKMLDDSKKLFDAFSSFGSVRDTRLFPNFWLGVVSGLVALLAI
IGFVWSSVRVRTREQDVRYQSQVEFNSRNQQAIMRLLDEISSLGEGDLTVKASVTEDMTGAIADAINYAVDELRHLVTTI
NDTSAKVAVSTQETQATAMQLAEAAGQQANQITTASERISEIAASIEQVSRNSTESAEVAQRSVVIAAEGAGVVRETIQG
MDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRGFAVVADEVQRLAERTSSATRR
IEGLVQTIQADTNEAVSSMEQTTSEVVSGARLAEDAGTALTEIERVSNALNNLIKNISIAAHQQSAAATDITQTMGVIRQ
ITSQTSQGAEQTAESIGNLAQLAADLRRSVADFKLPA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=283, Percent_Identity=30.0353356890459, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788195, Length=280, Percent_Identity=29.6428571428571, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787690, Length=317, Percent_Identity=28.391167192429, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI2367378, Length=321, Percent_Identity=28.0373831775701, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1789453, Length=346, Percent_Identity=28.0346820809249, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 71810; Mature: 71679

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAPDARKTNKLGSVSTSFWLGLLVLSMIVFGANTGVATWQSSHLAGAGVSAADLQVLS
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QQLANQGREAVSGDTKAFAAFKETKGRIDSTVNDLDGRYGQEGPIASSMSQLKATWVPLS
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
KNADQVIASEQAVLAIAVNANRFSGSVPQLQAQLNEVVRAMTVSGAPASQIYNTLQQVVV
CCHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AGTMARRVTEIRAGEDDAVASGDALARDSVVFTQMLEGLRAGNEELGIAAVRNPAALSAL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEECCHHHHHHH
EQSQAQWTRMKRDVDAILANSRNLFAAQSSAAALTAGSSKMLDDSKKLFDAFSSFGSVRD
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHEECCCHHHHHHHHHHHHHHHHHCCCHH
TRLFPNFWLGVVSGLVALLAIIGFVWSSVRVRTREQDVRYQSQVEFNSRNQQAIMRLLDE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISSLGEGDLTVKASVTEDMTGAIADAINYAVDELRHLVTTINDTSAKVAVSTQETQATAM
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHH
QLAEAAGQQANQITTASERISEIAASIEQVSRNSTESAEVAQRSVVIAAEGAGVVRETIQ
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHHHH
GMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCC
FAVVADEVQRLAERTSSATRRIEGLVQTIQADTNEAVSSMEQTTSEVVSGARLAEDAGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTEIERVSNALNNLIKNISIAAHQQSAAATDITQTMGVIRQITSQTSQGAEQTAESIGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AQLAADLRRSVADFKLPA
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STAPDARKTNKLGSVSTSFWLGLLVLSMIVFGANTGVATWQSSHLAGAGVSAADLQVLS
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QQLANQGREAVSGDTKAFAAFKETKGRIDSTVNDLDGRYGQEGPIASSMSQLKATWVPLS
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
KNADQVIASEQAVLAIAVNANRFSGSVPQLQAQLNEVVRAMTVSGAPASQIYNTLQQVVV
CCHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AGTMARRVTEIRAGEDDAVASGDALARDSVVFTQMLEGLRAGNEELGIAAVRNPAALSAL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEECCHHHHHHH
EQSQAQWTRMKRDVDAILANSRNLFAAQSSAAALTAGSSKMLDDSKKLFDAFSSFGSVRD
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHEECCCHHHHHHHHHHHHHHHHHCCCHH
TRLFPNFWLGVVSGLVALLAIIGFVWSSVRVRTREQDVRYQSQVEFNSRNQQAIMRLLDE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISSLGEGDLTVKASVTEDMTGAIADAINYAVDELRHLVTTINDTSAKVAVSTQETQATAM
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHH
QLAEAAGQQANQITTASERISEIAASIEQVSRNSTESAEVAQRSVVIAAEGAGVVRETIQ
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHHHH
GMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCC
FAVVADEVQRLAERTSSATRRIEGLVQTIQADTNEAVSSMEQTTSEVVSGARLAEDAGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTEIERVSNALNNLIKNISIAAHQQSAAATDITQTMGVIRQITSQTSQGAEQTAESIGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AQLAADLRRSVADFKLPA
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]