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The map label for this gene is aspS [H]

Identifier: 84623197

GI number: 84623197

Start: 1696018

End: 1697784

Strand: Direct

Name: aspS [H]

Synonym: XOO_1540

Alternate gene names: 84623197

Gene position: 1696018-1697784 (Clockwise)

Preceding gene: 84623195

Following gene: 84623198

Centisome position: 34.33

GC content: 65.03

Gene sequence:

>1767_bases
ATGCGTACCCACTTCTGCGGCCTGGTCGATGAGACCTTGATCGGCCAAACCGTCACTCTCGCCGGCTGGACCGACGTGGC
CCGTAACCTGGGCGGGGTGTGCTTCATCGACCTGCGCGACCACGAAGGCATCGTGCAGGTGACGGTGGAACCGGTGGCCG
GCGACGACGCCAGCGCAGAGCTGTTCAAGGTGGCCGCCAGCCTGGGCTACGAGGACGTGTTGCAGGTCGAAGGCGTTGTG
CGCGCGCGCCATGCGGTGAACGACAAGCTGCGCACCGGCAAGGTGGAAGTGATCGCCACGCGCATCAGCATCCTCAACAA
GGCCGCGCCGCTGCCGTTCCACGCGCATGAGAATCCGGGCGAAGAAACCCGCCTGAAGTACCGCTATCTGGACCTGCGTC
GCCCGGAAATGCAGCGCATGCAGCGCACCCGCATCAAGCTGGTGCAGGCGCTGCGCCGGCATCTGGATGCGCGCGATTTT
CAGGACATCGAAACCCCGATCCTGACCAAGGCCACCCCGGAAGGCGCGCGCGACTTCCTGGTGCCGGCGCGCATGCATCC
GGGCGAGTTCTATGCGCTGCCGCAGAGCCCGCAGCTGTTCAAGCAGATCCTGATGGTGGCCGGCTTCGACCGTTACTACC
AGATCGCGCGCTGCTTCCGCGACGAAGCGCTACGCGCCGACCGCCAGCTGGAATTCACCCAGCTCGACATGGAATTCGCT
TTCGTGCGCGAACGCGACGTGCAGGATTTTGTCGAAGACATGATGCGCGCCATCTTCAAGGAAGTGGTGGATGTCGATCT
GGCCGCGCAGTTCCCGCGCATGACCTGGGCCGAGGCGATGCGTCGCTACGGTTCGGACAAGCCGGACCTGCGCATCGCCC
TGGAATTGGTCGACGTGGCGGAACTGGTCAAATCCAGCGAATTTCCGGTGTTCACTGCCGCCGCCAACGATGCCGATGGC
CGCGTGGCTGCGCTGCGCATCCCCGGCGGCGCCACGCTGTCGCGCAAGCAGATCGACGACTACGCCGCGCATGCCGCCAA
ATATGGTGCCAAGGGCCTGGCCTATATCAAGCTGTCGGAGACCGGCGAGGTCAGCTCGCCGATCGCCAAGTTCTTCAGCG
AAGAAGCCTTCGCCGCGCTGCTCAAGCATGTAGGCGCCGGCAATGGCGACATCGTGTTCTTCGGTGCCGGTGGCTACACC
AAGGTGTCCGACTTCATGGGCGCGCTGCGCCTGAAGGCCGGCAAGGAGTTCGATCTGGTCGCAGAGGGCTGGGCGCCGCT
GTGGGTCACTGATTTCCCGATGTTCGAATGGGACGAGGAAGCGCAGCGCTACGTGGCCCTGCATCACCCCTTCACCGCAC
CGGCGGTGGACGATATCGCCGACCTGCGCGCCAACGCCCGCACCGCGGTCTCGCGCGGTTACGACATGGTGCTCAACGGT
AATGAAATCGGCGGCGGCTCGATCCGTATCCATCGCCCCGACATGCAGAGCGCGGTGTTCGAGTTGCTGGGCATCGGTGC
AGAAGAAGCGCGGGCCAAGTTCGGCTTCCTGCTGGATGCGTTGAACTATGGCGCCCCGCCGCACGGTGGCATCGCGTTCG
GTATCGACCGCATCGCCGCGCTGATGGCCGGCACCGAGTCCATCCGCGACGTGATCCCGTTCCCCAAGACCACCGGCGCG
CAGGATCTGATGACCGACGCCCCGTCGCCGATCGCCGCAGACCAACTTGCCGAAGTGCACGTGCAGGTCCGCTCGAAGCA
GGTCTGA

Upstream 100 bases:

>100_bases
GCGCCCGTGAGCGCCTGCGCATTTGCTCCTGTCGCCCGCCGGCCCCAGAATATCCGGCTTTCCGGCGCTGTCCTGCGTCG
GGTTTTCAGCGGAGCTTTCG

Downstream 100 bases:

>100_bases
TCCGCGATCAAGCACGGCCACGCCTAGCGCGTGGCCGTGTTATTTATGCGCGGCGAGCGACCTTCCGGGAACGGCATCGC
TTGGCCAATCTGCGCGGCGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRTGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMMRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVAELVKSSEFPVFTAAANDADG
RVAALRIPGGATLSRKQIDDYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEEAFAALLKHVGAGNGDIVFFGAGGYT
KVSDFMGALRLKAGKEFDLVAEGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRSKQV

Sequences:

>Translated_588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRTGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMMRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVAELVKSSEFPVFTAAANDADG
RVAALRIPGGATLSRKQIDDYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEEAFAALLKHVGAGNGDIVFFGAGGYT
KVSDFMGALRLKAGKEFDLVAEGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRSKQV
>Mature_588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRTGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMMRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVAELVKSSEFPVFTAAANDADG
RVAALRIPGGATLSRKQIDDYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEEAFAALLKHVGAGNGDIVFFGAGGYT
KVSDFMGALRLKAGKEFDLVAEGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRSKQV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=40.2337228714524, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI45439306, Length=255, Percent_Identity=31.3725490196078, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI5031815, Length=288, Percent_Identity=26.0416666666667, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI194272210, Length=288, Percent_Identity=26.0416666666667, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=55.6492411467116, Blast_Score=648, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=327, Percent_Identity=27.82874617737, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790571, Length=310, Percent_Identity=28.7096774193548, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=35.9531772575251, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17551876, Length=266, Percent_Identity=26.6917293233083, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=264, Percent_Identity=24.6212121212121, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=645, Percent_Identity=32.2480620155039, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=30.4, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=257, Percent_Identity=29.1828793774319, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=174, Percent_Identity=24.7126436781609, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=37.3134328358209, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=27.9245283018868, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=27.34375, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=27.34375, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65072; Mature: 65072

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAE
CCCCHHCHHHHHHCCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCHHH
LFKVAASLGYEDVLQVEGVVRARHAVNDKLRTGKVEVIATRISILNKAAPLPFHAHENPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCC
EETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
HHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCCCCCHHCC
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHH
FVRERDVQDFVEDMMRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHH
ELVKSSEFPVFTAAANDADGRVAALRIPGGATLSRKQIDDYAAHAAKYGAKGLAYIKLSE
HHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
TGEVSSPIAKFFSEEAFAALLKHVGAGNGDIVFFGAGGYTKVSDFMGALRLKAGKEFDLV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEE
AEGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
ECCCCCEEEECCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
CEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAADQLAEVHVQVRSKQV
HHHCHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAE
CCCCHHCHHHHHHCCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCHHH
LFKVAASLGYEDVLQVEGVVRARHAVNDKLRTGKVEVIATRISILNKAAPLPFHAHENPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCC
EETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
HHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCCCCCHHCC
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHH
FVRERDVQDFVEDMMRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHH
ELVKSSEFPVFTAAANDADGRVAALRIPGGATLSRKQIDDYAAHAAKYGAKGLAYIKLSE
HHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
TGEVSSPIAKFFSEEAFAALLKHVGAGNGDIVFFGAGGYTKVSDFMGALRLKAGKEFDLV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEE
AEGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
ECCCCCEEEECCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
CEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAADQLAEVHVQVRSKQV
HHHCHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]