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The map label for this gene is insH10 [H]

Identifier: 84623114

GI number: 84623114

Start: 1588266

End: 1589249

Strand: Direct

Name: insH10 [H]

Synonym: XOO_1457

Alternate gene names: 84623114

Gene position: 1588266-1589249 (Clockwise)

Preceding gene: 84623110

Following gene: 84623115

Centisome position: 32.15

GC content: 58.84

Gene sequence:

>984_bases
ATGCAGCTTTCTTTCGGCAATGCGGAGTACAACGGCAAGCGTAAGCGGACACGGCGCGAGGTGTTCTTGTCCGAGATGAA
TCAGGTGGTGCCTTGGAAAGGCCTGCTGGCGTTGATCGAGCCGCACTACCCGACGTCGGGCCAGCCGGGCCGGCAGCCAT
ATCCACTGGAGATGATGCTGCGCATCCATTTTTTGCAGCAGTGGAACGCACTGAGCGATCCGGCAGCGGAAGAAGCGTTG
TACGACACGGTGTCGATGCGCCGTTTCGCCAAGATAGGTGGTCTGGATGAGGTGCCTGACGAGACCACGATTCTCAACTT
CCGCCACTTGCTGGAGCGGCATGATTTGGCGCGCAAGCTGTTTGATCGGGTCAACGCACACTTGTTGCGCAAGGGGCAGA
GCCTGCGAGGCGGCACGATCGTGGACGCCACGATCATTGCTGCGCCCAGTTCGATCAAGAACAAGGACGGTGCGCGCACC
CCTGAAATGCATCAGACCAAAAAGGGCAACCAGTACTACTTCGGGATGAAAGCGCACATTGGCGTGGACGAGGAGTCTGG
CCTGGTGCACCACGTGGAATGCACAGCAGCCAACGTGGCCGACATCACGAAGGCGCACAAGCTGCTACACGGCAAGGAGG
GCACGGTGAGCGGCGACAGCGGCTACACCGGGCTGGACAAGCACGAAGAGTTGAGGACCAAGCGCAAGCTACGCTACCTG
ATCGCCGAGAAGCCCTCGAAGCTGAAGCAGATCAAGAGCAAACGCGAACTGAAGTTGACCAAGCGCTGGGAGCACGCTAA
AGCCAGCCTGCATGCGAAGGTGGAACATCTTTTTTCGGGTGATCAAGCGCCAGTTTGGCTACGCCAAGGTGCGCTATCGC
GGTTTGGCGAAGAACACCGCACAGGTGCTGACGCTGTTTGCACTGTCGAATCTGTGGCTGACGCGCAAAGAGTTAATGCC
TGCCGCAGGGAGGTGCGCCTGTAA

Upstream 100 bases:

>100_bases
CACAAAGGTACGATTATTTGTAACACAGTAGAATTAAGGAGCCTCTGAACAACTCCCTTTGATCATGCAACAATGGTATA
GAGCCAACGGGACAACGACG

Downstream 100 bases:

>100_bases
CCCGGGCAATATCCCGAAAACGCGCCGAAGGCGGAAAAAATCAGTGGTTTGAGCGCCCTCAGCGCGGCGGATGTGGCTTG
CTTCGTTTTCCGGCCCCGTT

Product: ISXoo6 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQLSFGNAEYNGKRKRTRREVFLSEMNQVVPWKGLLALIEPHYPTSGQPGRQPYPLEMMLRIHFLQQWNALSDPAAEEAL
YDTVSMRRFAKIGGLDEVPDETTILNFRHLLERHDLARKLFDRVNAHLLRKGQSLRGGTIVDATIIAAPSSIKNKDGART
PEMHQTKKGNQYYFGMKAHIGVDEESGLVHHVECTAANVADITKAHKLLHGKEGTVSGDSGYTGLDKHEELRTKRKLRYL
IAEKPSKLKQIKSKRELKLTKRWEHAKASLHAKVEHLFSGDQAPVWLRQGALSRFGEEHRTGADAVCTVESVADAQRVNA
CRREVRL

Sequences:

>Translated_327_residues
MQLSFGNAEYNGKRKRTRREVFLSEMNQVVPWKGLLALIEPHYPTSGQPGRQPYPLEMMLRIHFLQQWNALSDPAAEEAL
YDTVSMRRFAKIGGLDEVPDETTILNFRHLLERHDLARKLFDRVNAHLLRKGQSLRGGTIVDATIIAAPSSIKNKDGART
PEMHQTKKGNQYYFGMKAHIGVDEESGLVHHVECTAANVADITKAHKLLHGKEGTVSGDSGYTGLDKHEELRTKRKLRYL
IAEKPSKLKQIKSKRELKLTKRWEHAKASLHAKVEHLFSGDQAPVWLRQGALSRFGEEHRTGADAVCTVESVADAQRVNA
CRREVRL
>Mature_327_residues
MQLSFGNAEYNGKRKRTRREVFLSEMNQVVPWKGLLALIEPHYPTSGQPGRQPYPLEMMLRIHFLQQWNALSDPAAEEAL
YDTVSMRRFAKIGGLDEVPDETTILNFRHLLERHDLARKLFDRVNAHLLRKGQSLRGGTIVDATIIAAPSSIKNKDGART
PEMHQTKKGNQYYFGMKAHIGVDEESGLVHHVECTAANVADITKAHKLLHGKEGTVSGDSGYTGLDKHEELRTKRKLRYL
IAEKPSKLKQIKSKRELKLTKRWEHAKASLHAKVEHLFSGDQAPVWLRQGALSRFGEEHRTGADAVCTVESVADAQRVNA
CRREVRL

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=278, Percent_Identity=53.9568345323741, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI1788303, Length=278, Percent_Identity=53.5971223021583, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1789921, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1789612, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1789355, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1788518, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1788342, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1787592, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1786877, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1786764, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1786453, Length=278, Percent_Identity=53.2374100719424, Blast_Score=297, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37010; Mature: 37010

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSFGNAEYNGKRKRTRREVFLSEMNQVVPWKGLLALIEPHYPTSGQPGRQPYPLEMML
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
RIHFLQQWNALSDPAAEEALYDTVSMRRFAKIGGLDEVPDETTILNFRHLLERHDLARKL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FDRVNAHLLRKGQSLRGGTIVDATIIAAPSSIKNKDGARTPEMHQTKKGNQYYFGMKAHI
HHHHHHHHHHCCCCCCCCEEEEHEEEECCCHHCCCCCCCCCHHHHCCCCCEEEEEEEEEE
GVDEESGLVHHVECTAANVADITKAHKLLHGKEGTVSGDSGYTGLDKHEELRTKRKLRYL
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IAEKPSKLKQIKSKRELKLTKRWEHAKASLHAKVEHLFSGDQAPVWLRQGALSRFGEEHR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TGADAVCTVESVADAQRVNACRREVRL
CCCCCEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLSFGNAEYNGKRKRTRREVFLSEMNQVVPWKGLLALIEPHYPTSGQPGRQPYPLEMML
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
RIHFLQQWNALSDPAAEEALYDTVSMRRFAKIGGLDEVPDETTILNFRHLLERHDLARKL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FDRVNAHLLRKGQSLRGGTIVDATIIAAPSSIKNKDGARTPEMHQTKKGNQYYFGMKAHI
HHHHHHHHHHCCCCCCCCEEEEHEEEECCCHHCCCCCCCCCHHHHCCCCCEEEEEEEEEE
GVDEESGLVHHVECTAANVADITKAHKLLHGKEGTVSGDSGYTGLDKHEELRTKRKLRYL
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IAEKPSKLKQIKSKRELKLTKRWEHAKASLHAKVEHLFSGDQAPVWLRQGALSRFGEEHR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TGADAVCTVESVADAQRVNACRREVRL
CCCCCEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]