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The map label for this gene is dnaB [H]

Identifier: 84623071

GI number: 84623071

Start: 1538462

End: 1539892

Strand: Direct

Name: dnaB [H]

Synonym: XOO_1414

Alternate gene names: 84623071

Gene position: 1538462-1539892 (Clockwise)

Preceding gene: 84623070

Following gene: 84623072

Centisome position: 31.14

GC content: 61.91

Gene sequence:

>1431_bases
ATGTCCGCTCGTCCTGGTTTCCGTTCCAAGCGCAATCGTGATCGCGACGACGACGATTACGATCGTCCCGAGCCGCGTCT
GGACCAGCTGCGGGTGCCGCCGCATTCGGTCGAGGCCGAGCAGGCTGTGCTGGGTGGCTTGATGCTGGCGCCGGACGCGT
TCGACCGGGTCAACGATCAGCTCACCGAAAACGACTTCTACCGACGCGATCATCGGCTGATCTACCGCGCGATTCGCGAA
TTGAACCAAAAAGATCGCCCATTCGACGCAGTGACCCTGGGCGAATGGTTCGAATCGCAGGGCAAGCTGGAGCAGGTGGG
CGATGGCGCCTACCTGATCGAGCTGGCCAGCACCACGCCGTCGGCAGCCAACATCGCCGCGTATGCGGAAATCGTGCGCG
ACAAGGCGGTGCTGCGGCAGTTGATCGATGTGGGCACCACGATCGTCAACGATGGCTTCCAGCCAGAGGGCCGCGACAGC
ATCGAGTTGCTGTCCTCTGCGGAAAAGGCCGTGTTCAAGATCGCCGAAGCCGGCGCGCGCGGGCGGGCCGATTTCGTGGC
GATGCCCGGCGCCTTGAAGGATGCCTTCGAAGAGCTGCGCAACCGTTTCGAAAACGGCGGCAACATCACCGGCTTGCCGA
CCGGCTATAACGATTTCGATGCGATGACGGCCGGCCTGCAGCCGACCGACTTGATCATCCTGGCCGCGCGTCCGGCCATG
GGCAAGACCACCTTCGCGCTGAACATTGCCGAATACGCCGCGATCAAGTCCAAGAAGGGCGTGGCGGTGTTTTCGATGGA
AATGTCGGCCTCGCAGCTGGCGATGCGCCTGATCTCCTCCAACGGTCGCATCAATGCGCAGCGTCTGCGGACCGGTGCGC
TGGAAGACGAGGATTGGGCACGCGTGACCGGTGCGATCAAGATGCTGAAAGAAACCAAGATCTTCATCGACGACACCCCG
GGCGTGTCGCCGGAAGTGCTGCGTTCCAAGTGCCGCCGCCTCAAGCGCGAACACGACCTGGGCTTGATCGTCATCGACTA
CCTGCAGCTGATGTCGGTGCCCGGCAACAGCGAAAACCGCGCGACCGAAATTTCGGAAATTTCGCGTGGCCTGAAGGGGC
TTGCGAAGGAACTCAACGTGCCGGTGATCGCGTTGTCGCAGCTCAACCGCTCGCTGGAAACGCGGACCGATAAGCGCCCG
GTGATGGCCGACTTGCGCGAATCCGGCGCAATCGAGCAGGACGCGGACATGATCGTCTTTATCTACCGCGACGATTACTA
CAACAAGGAAAATTCGCCGGACAAGGGCCTGGCCGAGATCATCATCGGCAAGCACCGCGGCGGCCCGACCGGCTCGTGCA
AGCTCAAGTTCTTCGGTGAGTACACCCGCTTCGACAATCTGGCGCACGACTCGGTGGGCAGTTTCGAGTAA

Upstream 100 bases:

>100_bases
CCGCCTATACACCAGTTATCCACAGAGTTGTGCATGGTCGTCGGCGTCGCCGATGACTATGCTTCCTGCCCTTGTCTGCC
CGCGTCAGGTTTGTCTGTCC

Downstream 100 bases:

>100_bases
GCGCAGTGGACCCGCTGGGCGGCCATCCGTCGCCCGGCATCGCGCCATCGTTGCGGCCGCTGGCTTATGCTGCGCAGCCT
CATCGTTCGCTGCCGAGTTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSARPGFRSKRNRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQLTENDFYRRDHRLIYRAIRE
LNQKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTPSAANIAAYAEIVRDKAVLRQLIDVGTTIVNDGFQPEGRDS
IELLSSAEKAVFKIAEAGARGRADFVAMPGALKDAFEELRNRFENGGNITGLPTGYNDFDAMTAGLQPTDLIILAARPAM
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWARVTGAIKMLKETKIFIDDTP
GVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENRATEISEISRGLKGLAKELNVPVIALSQLNRSLETRTDKRP
VMADLRESGAIEQDADMIVFIYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE

Sequences:

>Translated_476_residues
MSARPGFRSKRNRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQLTENDFYRRDHRLIYRAIRE
LNQKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTPSAANIAAYAEIVRDKAVLRQLIDVGTTIVNDGFQPEGRDS
IELLSSAEKAVFKIAEAGARGRADFVAMPGALKDAFEELRNRFENGGNITGLPTGYNDFDAMTAGLQPTDLIILAARPAM
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWARVTGAIKMLKETKIFIDDTP
GVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENRATEISEISRGLKGLAKELNVPVIALSQLNRSLETRTDKRP
VMADLRESGAIEQDADMIVFIYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE
>Mature_475_residues
SARPGFRSKRNRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQLTENDFYRRDHRLIYRAIREL
NQKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTPSAANIAAYAEIVRDKAVLRQLIDVGTTIVNDGFQPEGRDSI
ELLSSAEKAVFKIAEAGARGRADFVAMPGALKDAFEELRNRFENGGNITGLPTGYNDFDAMTAGLQPTDLIILAARPAMG
KTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWARVTGAIKMLKETKIFIDDTPG
VSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENRATEISEISRGLKGLAKELNVPVIALSQLNRSLETRTDKRPV
MADLRESGAIEQDADMIVFIYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=452, Percent_Identity=53.9823008849557, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52897; Mature: 52766

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARPGFRSKRNRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCHHCCCHHHHHHHHH
LTENDFYRRDHRLIYRAIRELNQKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTP
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCHHHCCCCEEEEEEECCCC
SAANIAAYAEIVRDKAVLRQLIDVGTTIVNDGFQPEGRDSIELLSSAEKAVFKIAEAGAR
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRADFVAMPGALKDAFEELRNRFENGGNITGLPTGYNDFDAMTAGLQPTDLIILAARPAM
CCCCEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHCCCCCCCEEEEEECCCC
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWA
CCCEEEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEHHHHHCCCCCCHHHH
RVTGAIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENR
HHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCH
ATEISEISRGLKGLAKELNVPVIALSQLNRSLETRTDKRPVMADLRESGAIEQDADMIVF
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEE
IYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE
EEECCCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEEECHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
SARPGFRSKRNRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQ
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCHHCCCHHHHHHHHH
LTENDFYRRDHRLIYRAIRELNQKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTP
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCHHHCCCCEEEEEEECCCC
SAANIAAYAEIVRDKAVLRQLIDVGTTIVNDGFQPEGRDSIELLSSAEKAVFKIAEAGAR
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRADFVAMPGALKDAFEELRNRFENGGNITGLPTGYNDFDAMTAGLQPTDLIILAARPAM
CCCCEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHCCCCCCCEEEEEECCCC
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWA
CCCEEEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEHHHHHCCCCCCHHHH
RVTGAIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENR
HHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCH
ATEISEISRGLKGLAKELNVPVIALSQLNRSLETRTDKRPVMADLRESGAIEQDADMIVF
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEE
IYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE
EEECCCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEEECHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]