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The map label for this gene is murD [H]

Identifier: 84622977

GI number: 84622977

Start: 1440568

End: 1441974

Strand: Direct

Name: murD [H]

Synonym: XOO_1320

Alternate gene names: 84622977

Gene position: 1440568-1441974 (Clockwise)

Preceding gene: 84622976

Following gene: 84622978

Centisome position: 29.16

GC content: 66.45

Gene sequence:

>1407_bases
GTGCGAATTTCGCAGTTTGAAGGCAAGGCGGTTGCGTTGTGGGGCTGGGGACGCGAAGGACGCGGGGCGTATCGTGCGCT
GCGCGCGCAGCTGCCCACCCAATCGCTGACGATGTTCTGTAACGCTGAAGAGGTGCGCGAACTCGAATCGCTTGCAGACG
CAGCCTTGCATGTGGAAACCGATGCCAGCGCGCAGGCGTTGGGGCGATTCGAGATTGTGGTGAAGTCGCCCGGCATCAGC
CCGTATCGCGCTGAGGCACTGGCCGCCGCCGCGCAGGGCACGCAATTCATTGGCGGCACCGCGCTGTGGTTTGCCGAGCA
CGCGCAGCCGGACGGCAGCGTGCCTGGCGCCATTTGCGTTACCGGCACCAAGGGCAAGAGCACCACCACTGCGTTGCTGG
CGCACTTGCTGCGCGTGGCCGGGCATCGCACTGCGTTGGTCGGCAATATCGGCCAGCCCCTGCTGGAAGTGCTGGCACCG
CAGCCGCCGCCGGCGTATTGGGCGATCGAGTTGTCCAGCTATCAAACCGGTGATGTCGGACGCAGCGGCGCGCGCCCGGA
GCTGGCGGTGGTGTTGAATCTATTTCCCGAGCATCTGGACTGGCACGGCGATGAGGCGCGCTATGTGCGCGACAAGCTGT
CGCTGGTGACCGAAGGCCGGCCGCGGATCGTCTTGCTCAACGCGGCCGACCCGCTTCTTGCCAGTTTGCAGCTGCCCGAC
AGCGAGGTGCTGTGGTTCAACCATCCCGAGGGCTGGCATTTGCGTGGCGATGTGGTCTATCGCGGCGAACAAGCCATTTT
CGATAGCGCTGACGTGCCGCTACCGGGCGTGCACAATCGCCGCAACCTGTGCGCGGTGCTGGCAGCGCTGGAAGCCCTGG
GCCTGGATGCTGAAGCACTGGCGCCGGCAGCCTTGAGCTTTCGTCCGTTGCCGAACCGCCTGCAGGTGCTCGGCAGCGTG
GACGGCATCAGCTACGTCAACGACTCGATCAGCACCACGCCGTATGCCTCGCTGGCCGCATTGGCGTGCTTCGCGCAGCG
TCGCGTTGCCTTGCTGGTCGGCGGGCACGACCGTGGCCTGGACTGGCACGATTTCGCCCGGCACATGGCGCAGCAGGCGC
CGCTGGAAATCGTCACCATGGCTGCCAATGGCCCGCGTATCCATGCGTTGCTGGCACCGCTGGCCGACGCCGGGCGCTTC
GGCTTGCATGCCGCAAACGATCTGGAACACGCCATGCAACTGGCGCGCGATGCGTTGGGCGGGCAGGGGGGCGTGGTGCT
GCTCTCGCCGGGGGCGCCCAGCTTCGGCGCCTATAGCGACTACGTTGCGCGCGGTCGGCACTTTGCCCAGCTGGCGGGGT
TCGACCCGGCGGCGATCAGCGCGATTCCCGGGTTGGGTGTGCATTAA

Upstream 100 bases:

>100_bases
GACATCCAGCCGCAGCTCGACCCGAATGACCTGCAGGTCGAGCCACTGATGGCCATCGAAGGCGAGCACCGGATTCCGCC
AGCGCTGTGGGAGCGCGTGC

Downstream 100 bases:

>100_bases
TCCGACTAACAGACCGACTGCGCTCATCGCTAGAAGTGCGGAGTCGGTGCTCGGAATCGGCAAGTACCGCTCGCTCTGGT
TTCTCTGCACCGTCCACATT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRISQFEGKAVALWGWGREGRGAYRALRAQLPTQSLTMFCNAEEVRELESLADAALHVETDASAQALGRFEIVVKSPGIS
PYRAEALAAAAQGTQFIGGTALWFAEHAQPDGSVPGAICVTGTKGKSTTTALLAHLLRVAGHRTALVGNIGQPLLEVLAP
QPPPAYWAIELSSYQTGDVGRSGARPELAVVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIVLLNAADPLLASLQLPD
SEVLWFNHPEGWHLRGDVVYRGEQAIFDSADVPLPGVHNRRNLCAVLAALEALGLDAEALAPAALSFRPLPNRLQVLGSV
DGISYVNDSISTTPYASLAALACFAQRRVALLVGGHDRGLDWHDFARHMAQQAPLEIVTMAANGPRIHALLAPLADAGRF
GLHAANDLEHAMQLARDALGGQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIPGLGVH

Sequences:

>Translated_468_residues
MRISQFEGKAVALWGWGREGRGAYRALRAQLPTQSLTMFCNAEEVRELESLADAALHVETDASAQALGRFEIVVKSPGIS
PYRAEALAAAAQGTQFIGGTALWFAEHAQPDGSVPGAICVTGTKGKSTTTALLAHLLRVAGHRTALVGNIGQPLLEVLAP
QPPPAYWAIELSSYQTGDVGRSGARPELAVVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIVLLNAADPLLASLQLPD
SEVLWFNHPEGWHLRGDVVYRGEQAIFDSADVPLPGVHNRRNLCAVLAALEALGLDAEALAPAALSFRPLPNRLQVLGSV
DGISYVNDSISTTPYASLAALACFAQRRVALLVGGHDRGLDWHDFARHMAQQAPLEIVTMAANGPRIHALLAPLADAGRF
GLHAANDLEHAMQLARDALGGQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIPGLGVH
>Mature_468_residues
MRISQFEGKAVALWGWGREGRGAYRALRAQLPTQSLTMFCNAEEVRELESLADAALHVETDASAQALGRFEIVVKSPGIS
PYRAEALAAAAQGTQFIGGTALWFAEHAQPDGSVPGAICVTGTKGKSTTTALLAHLLRVAGHRTALVGNIGQPLLEVLAP
QPPPAYWAIELSSYQTGDVGRSGARPELAVVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIVLLNAADPLLASLQLPD
SEVLWFNHPEGWHLRGDVVYRGEQAIFDSADVPLPGVHNRRNLCAVLAALEALGLDAEALAPAALSFRPLPNRLQVLGSV
DGISYVNDSISTTPYASLAALACFAQRRVALLVGGHDRGLDWHDFARHMAQQAPLEIVTMAANGPRIHALLAPLADAGRF
GLHAANDLEHAMQLARDALGGQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIPGLGVH

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=388, Percent_Identity=27.5773195876289, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49747; Mature: 49747

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISQFEGKAVALWGWGREGRGAYRALRAQLPTQSLTMFCNAEEVRELESLADAALHVET
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHEEEEC
DASAQALGRFEIVVKSPGISPYRAEALAAAAQGTQFIGGTALWFAEHAQPDGSVPGAICV
CCCHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCEEEE
TGTKGKSTTTALLAHLLRVAGHRTALVGNIGQPLLEVLAPQPPPAYWAIELSSYQTGDVG
ECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RSGARPELAVVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIVLLNAADPLLASLQLPD
CCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHEEECCC
SEVLWFNHPEGWHLRGDVVYRGEQAIFDSADVPLPGVHNRRNLCAVLAALEALGLDAEAL
CCEEEEECCCCCEEECCEEECCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
APAALSFRPLPNRLQVLGSVDGISYVNDSISTTPYASLAALACFAQRRVALLVGGHDRGL
CCHHHCCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCC
DWHDFARHMAQQAPLEIVTMAANGPRIHALLAPLADAGRFGLHAANDLEHAMQLARDALG
CHHHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
GQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIPGLGVH
CCCCEEEECCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MRISQFEGKAVALWGWGREGRGAYRALRAQLPTQSLTMFCNAEEVRELESLADAALHVET
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHEEEEC
DASAQALGRFEIVVKSPGISPYRAEALAAAAQGTQFIGGTALWFAEHAQPDGSVPGAICV
CCCHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCEEEE
TGTKGKSTTTALLAHLLRVAGHRTALVGNIGQPLLEVLAPQPPPAYWAIELSSYQTGDVG
ECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RSGARPELAVVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIVLLNAADPLLASLQLPD
CCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHEEECCC
SEVLWFNHPEGWHLRGDVVYRGEQAIFDSADVPLPGVHNRRNLCAVLAALEALGLDAEAL
CCEEEEECCCCCEEECCEEECCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
APAALSFRPLPNRLQVLGSVDGISYVNDSISTTPYASLAALACFAQRRVALLVGGHDRGL
CCHHHCCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCC
DWHDFARHMAQQAPLEIVTMAANGPRIHALLAPLADAGRFGLHAANDLEHAMQLARDALG
CHHHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
GQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIPGLGVH
CCCCEEEECCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]