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The map label for this gene is cobS [H]

Identifier: 84622900

GI number: 84622900

Start: 1353094

End: 1353828

Strand: Direct

Name: cobS [H]

Synonym: XOO_1243

Alternate gene names: 84622900

Gene position: 1353094-1353828 (Clockwise)

Preceding gene: 84622899

Following gene: 84622908

Centisome position: 27.39

GC content: 68.98

Gene sequence:

>735_bases
ATGATCGAAGGCTTGCTCGCCGCAGTCGGGTTTCTGACCCGCATGCCGGTGCCCGGCTGGGTGTTCGCGCGGCCCGGTGC
GCAGGCGCGCTCGTTGCTGTGGTATCCACTGGTCGGCGTGCTGATCGGCGCAGTATTGAGCGCGCTGGCGTGGTGCCTGC
GCGATGTGGCGCCGCTGCTGGGAGCCGCGTTGCTGCTGAGCAGCTGGGTCTGGCTTACCGGCGCCTTGCATATCGATGGC
TTGGCAGACACGGCCGATGCTTGGGTTGGCGGCATGGGCGATCGCATGCGTACGCTGGCGATCATGAAAGACCCCACCAG
TGGCCCGATGGCGCTGGCTGCGGTGGTGTTGGCGCTGCTGCTCAAGTGCGCGGCGCTGGCCACGCTGTTGCCCGCTGCGT
CCGACACCGTGTGGCTGGCGCCGCTGCTGGCACGCACGGCGTTGGTGGCGGCATTTGTGACCACGCCCTACGTGCGTGCC
GGCGGGCTTGGTAGTGGCTTGGTCGGCAGTGCGCGTGCGGGCTTGCTGCTGTCGTTGCTGCTCGGGCTGGGCTGCGCCTT
CGCCGCTGGGGTCGCGCGCGGGATGCTCGCCTGCGTTGTCGCTGGCGTGGTGTTCGTGCTGTGGCGTTGCGCTTGCCTGC
GGCGCCTGGGCGGCATGACCGGCGATACCTGCGGTGCGTTGGTGGAGGTGAGCGAAACTGCTGTGCTGGTGGCGTTGGCG
CTGCAAGCGAGCTGA

Upstream 100 bases:

>100_bases
GCATGACCGCGCTTGCTGTGCCGCATGCCTCGCTGCATCCGCTGGCGTGGCCGCCGGCCGCAGCGAACCCGGCAGCACTG
CCTGCGGTCTCCAGCGCCGG

Downstream 100 bases:

>100_bases
AGGTTACGGGTAAGACGATTGCAGTGTCCCTGGAGCGGCTACCAAAACCTAGCGAGCAGCTGTCAGGTCGGCGCGGCCGG
TGCTCGGTGCGGCATGTGCC

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIEGLLAAVGFLTRMPVPGWVFARPGAQARSLLWYPLVGVLIGAVLSALAWCLRDVAPLLGAALLLSSWVWLTGALHIDG
LADTADAWVGGMGDRMRTLAIMKDPTSGPMALAAVVLALLLKCAALATLLPAASDTVWLAPLLARTALVAAFVTTPYVRA
GGLGSGLVGSARAGLLLSLLLGLGCAFAAGVARGMLACVVAGVVFVLWRCACLRRLGGMTGDTCGALVEVSETAVLVALA
LQAS

Sequences:

>Translated_244_residues
MIEGLLAAVGFLTRMPVPGWVFARPGAQARSLLWYPLVGVLIGAVLSALAWCLRDVAPLLGAALLLSSWVWLTGALHIDG
LADTADAWVGGMGDRMRTLAIMKDPTSGPMALAAVVLALLLKCAALATLLPAASDTVWLAPLLARTALVAAFVTTPYVRA
GGLGSGLVGSARAGLLLSLLLGLGCAFAAGVARGMLACVVAGVVFVLWRCACLRRLGGMTGDTCGALVEVSETAVLVALA
LQAS
>Mature_244_residues
MIEGLLAAVGFLTRMPVPGWVFARPGAQARSLLWYPLVGVLIGAVLSALAWCLRDVAPLLGAALLLSSWVWLTGALHIDG
LADTADAWVGGMGDRMRTLAIMKDPTSGPMALAAVVLALLLKCAALATLLPAASDTVWLAPLLARTALVAAFVTTPYVRA
GGLGSGLVGSARAGLLLSLLLGLGCAFAAGVARGMLACVVAGVVFVLWRCACLRRLGGMTGDTCGALVEVSETAVLVALA
LQAS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=34.9397590361446, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 24831; Mature: 24831

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEGLLAAVGFLTRMPVPGWVFARPGAQARSLLWYPLVGVLIGAVLSALAWCLRDVAPLL
CHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAALLLSSWVWLTGALHIDGLADTADAWVGGMGDRMRTLAIMKDPTSGPMALAAVVLALL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHH
LKCAALATLLPAASDTVWLAPLLARTALVAAFVTTPYVRAGGLGSGLVGSARAGLLLSLL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHH
LGLGCAFAAGVARGMLACVVAGVVFVLWRCACLRRLGGMTGDTCGALVEVSETAVLVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LQAS
HHCC
>Mature Secondary Structure
MIEGLLAAVGFLTRMPVPGWVFARPGAQARSLLWYPLVGVLIGAVLSALAWCLRDVAPLL
CHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAALLLSSWVWLTGALHIDGLADTADAWVGGMGDRMRTLAIMKDPTSGPMALAAVVLALL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHH
LKCAALATLLPAASDTVWLAPLLARTALVAAFVTTPYVRAGGLGSGLVGSARAGLLLSLL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHH
LGLGCAFAAGVARGMLACVVAGVVFVLWRCACLRRLGGMTGDTCGALVEVSETAVLVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LQAS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA