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The map label for this gene is trmD

Identifier: 84622878

GI number: 84622878

Start: 1329928

End: 1330707

Strand: Direct

Name: trmD

Synonym: XOO_1221

Alternate gene names: 84622878

Gene position: 1329928-1330707 (Clockwise)

Preceding gene: 84622877

Following gene: 84622879

Centisome position: 26.92

GC content: 67.05

Gene sequence:

>780_bases
GTGCGCATCGACGTCATCAGCCTGTTCCCCGAGTTCATCGCGCAATGCGCGGCGTTCGGCGTGGTCGGGCGGGCGCAGGA
GCGCGGCTTGCTGGAATTGCAGGGCTGGAATCCGCGCGAGCATGCACAGGGCAACTATCGCCGGGTGGACGACCGTCCGT
TCGGTGGCGGGCCCGGCATGGTGATGTTGATCGAGCCGCTGCGGGCCTGTCTGGATGCGGTGCAAGCCGCAGACGCGCGA
CCGGCACCGGTGATCTACTTCAGTCCGCAGGGGCGTCGGCTGACGCAAATGCTGGCGCGCGAGCTGGCGCAGCTGCCGCG
CATGGTGCTGTTGTGCGGGCGCTATGAAGGCGTGGACGAGCGCTTCCTGGCGCAGGCGGTGGATATGGAAATCTCCATCG
GCGACTACGTGTTGTCCGGCGGTGAACTGGGTGCGGCGGTTGTCGTGGACGTGGTGACGCGGTTGCAGGAGGGCGTGTTG
AACGACGCCGAATCGGCCGCTCAGGACAGTTTCGAAGGGCCGCAGGGCCTGCTGGACTGCCCGCACTACAGCCATCCGTC
GAGCCACGCCTGGGGCGATGTGCCGGAGGTGCTGCGGTCGGGCAATCATGCGGCCATTGCGCGCTGGCGCCGGCAGCAAT
CGCTGGGCCGGACCTGGTTGCGTCGCCCGGACCTGCTGGACGAGGCCGGGTTGGACAAGCATGATCGTCGTTTGCTGGAA
GAGTTTCGCCGCGAACTCGCCAAGGGCGACGAGGAATCAGGGTGCACACCTAGCCCTTGA

Upstream 100 bases:

>100_bases
ATCTGATCGTGGTCGACTGGGATCCGGATTTCTGATCAAAGCGGTGCCTCGGCGCTGCTTTTGCTTTACCCATTTCCGAT
TCCCGATTCCCGATTCTCCA

Downstream 100 bases:

>100_bases
AGATTTGATTGGGGTACAATATGCGGCTTGCTGGCCAGTCTTGCGTCTGGCCCTCCTACGAACCTCGAGCAATCGCCGTG
CGGCGACTGCCGGTCCAATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGMVMLIEPLRACLDAVQAADAR
PAPVIYFSPQGRRLTQMLARELAQLPRMVLLCGRYEGVDERFLAQAVDMEISIGDYVLSGGELGAAVVVDVVTRLQEGVL
NDAESAAQDSFEGPQGLLDCPHYSHPSSHAWGDVPEVLRSGNHAAIARWRRQQSLGRTWLRRPDLLDEAGLDKHDRRLLE
EFRRELAKGDEESGCTPSP

Sequences:

>Translated_259_residues
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGMVMLIEPLRACLDAVQAADAR
PAPVIYFSPQGRRLTQMLARELAQLPRMVLLCGRYEGVDERFLAQAVDMEISIGDYVLSGGELGAAVVVDVVTRLQEGVL
NDAESAAQDSFEGPQGLLDCPHYSHPSSHAWGDVPEVLRSGNHAAIARWRRQQSLGRTWLRRPDLLDEAGLDKHDRRLLE
EFRRELAKGDEESGCTPSP
>Mature_259_residues
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGMVMLIEPLRACLDAVQAADAR
PAPVIYFSPQGRRLTQMLARELAQLPRMVLLCGRYEGVDERFLAQAVDMEISIGDYVLSGGELGAAVVVDVVTRLQEGVL
NDAESAAQDSFEGPQGLLDCPHYSHPSSHAWGDVPEVLRSGNHAAIARWRRQQSLGRTWLRRPDLLDEAGLDKHDRRLLE
EFRRELAKGDEESGCTPSP

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=248, Percent_Identity=53.2258064516129, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_XANOM (Q2P651)

Other databases:

- EMBL:   AP008229
- RefSeq:   YP_450250.1
- ProteinModelPortal:   Q2P651
- SMR:   Q2P651
- STRING:   Q2P651
- GeneID:   3856575
- GenomeReviews:   AP008229_GR
- KEGG:   xom:XOO_1221
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q2P651
- ProtClustDB:   PRK00026
- BioCyc:   XORY342109:XOO1221-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28695; Mature: 28695

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHCCCCCEECCCCCCCCCCCH
VMLIEPLRACLDAVQAADARPAPVIYFSPQGRRLTQMLARELAQLPRMVLLCGRYEGVDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RFLAQAVDMEISIGDYVLSGGELGAAVVVDVVTRLQEGVLNDAESAAQDSFEGPQGLLDC
HHHHHHHCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCC
PHYSHPSSHAWGDVPEVLRSGNHAAIARWRRQQSLGRTWLRRPDLLDEAGLDKHDRRLLE
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
EFRRELAKGDEESGCTPSP
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHCCCCCEECCCCCCCCCCCH
VMLIEPLRACLDAVQAADARPAPVIYFSPQGRRLTQMLARELAQLPRMVLLCGRYEGVDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RFLAQAVDMEISIGDYVLSGGELGAAVVVDVVTRLQEGVLNDAESAAQDSFEGPQGLLDC
HHHHHHHCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCC
PHYSHPSSHAWGDVPEVLRSGNHAAIARWRRQQSLGRTWLRRPDLLDEAGLDKHDRRLLE
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
EFRRELAKGDEESGCTPSP
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA