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The map label for this gene is menE [H]

Identifier: 84622735

GI number: 84622735

Start: 1179235

End: 1180734

Strand: Direct

Name: menE [H]

Synonym: XOO_1078

Alternate gene names: 84622735

Gene position: 1179235-1180734 (Clockwise)

Preceding gene: 84622734

Following gene: 84622736

Centisome position: 23.87

GC content: 67.6

Gene sequence:

>1500_bases
ATGAGTGCACGATTCGGCCAATTGCTCGCTGAGCCACACGGCCATGCACTGCGGGTGCTGAGCGCCACCAGCAACCTGCA
CGCGCAGACCTTGGCCGACGCTGTCGCGCGGTTGGCAGCCTGCCTGCAGACCGCGGGGCTGACGCGGGTCGCCAGCCGGC
TGGACAACGGTCCGGACTGGTTGATCCTGGATCTGGCGCTACGTCTGATCGACGCCGTGCACGTGCCATTGCCGACGTTC
TTCTCCGATGGCCAGGTACTGCACGCGCTGACCTCCAGTGGCGCGCAATGCGTGGTGACCGATGCGGCCGCCGCCGCGCC
GCCCGGCACCAGCGGCCCACTCCCAGCGCTGCAGCAAGATGCGCTGCGCTGCTGGCGGGTGGCAGCGATCATCGAGCGAC
CTGTATTGCCGGATGCCACTGCCTGCATCACCTACACCTCCGGCACCACGGGTCGCCCCAAGGGCGTGTGCCTGTCGGCT
GACAGTTTGCTCACGGTGGCGGCGTCGCTGGTAGATGCCTCGGCTGCCATCGCACCGCGTCGGCATCTTTGCCTGATGCC
GTTGTCTACCTTGCTGGAGAACGTGGCCGGGCTGTACGCGACCTTGTTGTCCGGTGCGCAGGTGGCGCTGCCGTCGCTGG
CGCAGATCGGGTATACCGGCGCCAGCGGGCTGGATGTGCCTGCGCTGCTGCGCTGCCTGCACCAGTATCAACCCGAAAGC
GTGATCCTGGTGCCGCAGTTGTTGCTCGCCCTGGTCAGCGCTGCCGAGCACGGCGTGGCCCTGCCCGCATCGCTGCGCTA
TATCGCGGTCGGAGGCGGGCACATCGGCCCCAGCTTGCTCGCGCGTGCCGCTGCGTTGGAGCTGCCGGTATTCGAAGGCT
ACGGCCTAACCGAATGCGGCTCGGTGGTCTGTCTCAACCGGCCCGGCGCAGTGCGTGCCGGCAGCGTCGGTCAGCCATTG
GCGCATGCGCAGGTGCGGATCGTCGATGGCGAACTTCAAGTCGGTGGCGTGCAGGCACTTGGCTATCTGGGTGAGGACGC
GCCACCGCCGGGTCCGGTGCGCACCGGTGATCTGGGGCATGTCGACCCAGACGGTTTCGTGCACATCACCGGGCGTCGCA
AACATGTCTTCATTACCGCGTTCGGCCGCAATGTCTCGCCCGAATGGGTAGAAAGCGAGTTGCTGCAGCATCCGCTGCTT
GCGCAGGCGGTGGTGTGGGGCGAGGCGCAGGCCGACAACGTCGCGGTGCTATGGCCGCGCCGGCCCGACAGCGATGATGC
CGCGCTCGCCAAGGCGCTCTCCGAGGTCAATGCCGGCTTACCCGACTACGCACGCGTGGCGCGCTTCGTCCGCGCCGATG
CACCGTTCAACGCACGTGAGGGCCTGCTCACGGCCAACGGGCGCCCCCGCCGCGACGCGATCCTCGCGCGCTATCAATCC
GCCGTCGACCGCAGCTATCGGTTGCCGGCCACCGTCGTCTCTCAAGGAATTTCTCCATGA

Upstream 100 bases:

>100_bases
TCGCCGCCGGGCATGCCTTTTTGCTGGAGCGGCAGGCGCTGCGCCCGCAGGCCACACGCCCGGGCATCGACACGCTGCCG
TACCTGATGGGAGTGGGCGC

Downstream 100 bases:

>100_bases
CGTTTTATGCATCTTTGCTGGCGCAGACCCAGGCCGAACGCGAGCGCATGACCCAGGTGCCGATCATCCAGGCGGCACTG
GCCGGCAACATCGCGCGGGC

Product: putative long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSARFGQLLAEPHGHALRVLSATSNLHAQTLADAVARLAACLQTAGLTRVASRLDNGPDWLILDLALRLIDAVHVPLPTF
FSDGQVLHALTSSGAQCVVTDAAAAAPPGTSGPLPALQQDALRCWRVAAIIERPVLPDATACITYTSGTTGRPKGVCLSA
DSLLTVAASLVDASAAIAPRRHLCLMPLSTLLENVAGLYATLLSGAQVALPSLAQIGYTGASGLDVPALLRCLHQYQPES
VILVPQLLLALVSAAEHGVALPASLRYIAVGGGHIGPSLLARAAALELPVFEGYGLTECGSVVCLNRPGAVRAGSVGQPL
AHAQVRIVDGELQVGGVQALGYLGEDAPPPGPVRTGDLGHVDPDGFVHITGRRKHVFITAFGRNVSPEWVESELLQHPLL
AQAVVWGEAQADNVAVLWPRRPDSDDAALAKALSEVNAGLPDYARVARFVRADAPFNAREGLLTANGRPRRDAILARYQS
AVDRSYRLPATVVSQGISP

Sequences:

>Translated_499_residues
MSARFGQLLAEPHGHALRVLSATSNLHAQTLADAVARLAACLQTAGLTRVASRLDNGPDWLILDLALRLIDAVHVPLPTF
FSDGQVLHALTSSGAQCVVTDAAAAAPPGTSGPLPALQQDALRCWRVAAIIERPVLPDATACITYTSGTTGRPKGVCLSA
DSLLTVAASLVDASAAIAPRRHLCLMPLSTLLENVAGLYATLLSGAQVALPSLAQIGYTGASGLDVPALLRCLHQYQPES
VILVPQLLLALVSAAEHGVALPASLRYIAVGGGHIGPSLLARAAALELPVFEGYGLTECGSVVCLNRPGAVRAGSVGQPL
AHAQVRIVDGELQVGGVQALGYLGEDAPPPGPVRTGDLGHVDPDGFVHITGRRKHVFITAFGRNVSPEWVESELLQHPLL
AQAVVWGEAQADNVAVLWPRRPDSDDAALAKALSEVNAGLPDYARVARFVRADAPFNAREGLLTANGRPRRDAILARYQS
AVDRSYRLPATVVSQGISP
>Mature_498_residues
SARFGQLLAEPHGHALRVLSATSNLHAQTLADAVARLAACLQTAGLTRVASRLDNGPDWLILDLALRLIDAVHVPLPTFF
SDGQVLHALTSSGAQCVVTDAAAAAPPGTSGPLPALQQDALRCWRVAAIIERPVLPDATACITYTSGTTGRPKGVCLSAD
SLLTVAASLVDASAAIAPRRHLCLMPLSTLLENVAGLYATLLSGAQVALPSLAQIGYTGASGLDVPALLRCLHQYQPESV
ILVPQLLLALVSAAEHGVALPASLRYIAVGGGHIGPSLLARAAALELPVFEGYGLTECGSVVCLNRPGAVRAGSVGQPLA
HAQVRIVDGELQVGGVQALGYLGEDAPPPGPVRTGDLGHVDPDGFVHITGRRKHVFITAFGRNVSPEWVESELLQHPLLA
QAVVWGEAQADNVAVLWPRRPDSDDAALAKALSEVNAGLPDYARVARFVRADAPFNAREGLLTANGRPRRDAILARYQSA
VDRSYRLPATVVSQGISP

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807491, Length=443, Percent_Identity=25.5079006772009, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI57165412, Length=540, Percent_Identity=23.7037037037037, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI57165410, Length=540, Percent_Identity=23.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788107, Length=289, Percent_Identity=29.757785467128, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693145, Length=292, Percent_Identity=26.7123287671233, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556552, Length=446, Percent_Identity=23.9910313901345, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=416, Percent_Identity=24.7596153846154, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17510401, Length=440, Percent_Identity=23.6363636363636, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=26.2872628726287, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581924, Length=304, Percent_Identity=26.6447368421053, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648257, Length=373, Percent_Identity=26.8096514745308, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648260, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161076582, Length=373, Percent_Identity=25.4691689008043, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648253, Length=450, Percent_Identity=24.6666666666667, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653035, Length=260, Percent_Identity=27.3076923076923, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356947, Length=337, Percent_Identity=27.0029673590504, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18859661, Length=304, Percent_Identity=29.6052631578947, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52069; Mature: 51938

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARFGQLLAEPHGHALRVLSATSNLHAQTLADAVARLAACLQTAGLTRVASRLDNGPDW
CCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LILDLALRLIDAVHVPLPTFFSDGQVLHALTSSGAQCVVTDAAAAAPPGTSGPLPALQQD
EHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHH
ALRCWRVAAIIERPVLPDATACITYTSGTTGRPKGVCLSADSLLTVAASLVDASAAIAPR
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
RHLCLMPLSTLLENVAGLYATLLSGAQVALPSLAQIGYTGASGLDVPALLRCLHQYQPES
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
VILVPQLLLALVSAAEHGVALPASLRYIAVGGGHIGPSLLARAAALELPVFEGYGLTECG
EEHHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
SVVCLNRPGAVRAGSVGQPLAHAQVRIVDGELQVGGVQALGYLGEDAPPPGPVRTGDLGH
CEEEECCCCCCCCCCCCCCCCCCEEEEEECCEEECCHHHHHHCCCCCCCCCCCCCCCCCC
VDPDGFVHITGRRKHVFITAFGRNVSPEWVESELLQHPLLAQAVVWGEAQADNVAVLWPR
CCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECC
RPDSDDAALAKALSEVNAGLPDYARVARFVRADAPFNAREGLLTANGRPRRDAILARYQS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHH
AVDRSYRLPATVVSQGISP
HHCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
SARFGQLLAEPHGHALRVLSATSNLHAQTLADAVARLAACLQTAGLTRVASRLDNGPDW
CCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LILDLALRLIDAVHVPLPTFFSDGQVLHALTSSGAQCVVTDAAAAAPPGTSGPLPALQQD
EHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHH
ALRCWRVAAIIERPVLPDATACITYTSGTTGRPKGVCLSADSLLTVAASLVDASAAIAPR
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
RHLCLMPLSTLLENVAGLYATLLSGAQVALPSLAQIGYTGASGLDVPALLRCLHQYQPES
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
VILVPQLLLALVSAAEHGVALPASLRYIAVGGGHIGPSLLARAAALELPVFEGYGLTECG
EEHHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
SVVCLNRPGAVRAGSVGQPLAHAQVRIVDGELQVGGVQALGYLGEDAPPPGPVRTGDLGH
CEEEECCCCCCCCCCCCCCCCCCEEEEEECCEEECCHHHHHHCCCCCCCCCCCCCCCCCC
VDPDGFVHITGRRKHVFITAFGRNVSPEWVESELLQHPLLAQAVVWGEAQADNVAVLWPR
CCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECC
RPDSDDAALAKALSEVNAGLPDYARVARFVRADAPFNAREGLLTANGRPRRDAILARYQS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHH
AVDRSYRLPATVVSQGISP
HHCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA