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The map label for this gene is rihA [H]

Identifier: 84622706

GI number: 84622706

Start: 1142439

End: 1143377

Strand: Direct

Name: rihA [H]

Synonym: XOO_1049

Alternate gene names: 84622706

Gene position: 1142439-1143377 (Clockwise)

Preceding gene: 84622705

Following gene: 84622707

Centisome position: 23.13

GC content: 66.99

Gene sequence:

>939_bases
ATGACTGACAAGATACCGCTGCTGATCGACACCGACCCGGGCGTAGACGACGCGCTGGCGCTGCTGATGGCTTTCAACGA
CGCCCGCCATGAGGTGGTCGGCCTGACCATCGCTGCCGGTAACGTTGGCCTGGAGCACACCGTGCGCAACGCCTTGAAGG
TCTGCGAGATCGCCGGTCGCGCCGACGTGCCGGTCTACGCCGGCTGCTCGCAGCCGTTGCTGCACCCGTCGGTGGACGCG
GCCCACGTGCACGGTCTCGACGGCTTCGGCGATGTCGGTCTGCCACCGGCCAAGCGCACTGCCGAAGCCGAACACGCCGC
ACTCGCCATCCTGCGGCTGTCGCACCAACATGCCGGCAAATTGCTGTTGGTTGCGCTTGGCCCGCTGACCAATCTTGCCC
TGGCACTGATCCTGGACCCGACCCTGCCAACGCGCGTGGCGCGCTTGGTGGTGATGGGCGGGGCGCTGACCGGGCACGGC
AACATCACCGCCGCCGCCGAGTTCAATATCGGTTTCGACCCGGAAGCGGCGCATATCGTGTTCCGCGGCTTCCCGCAGTT
CGACGTCGCCGACTGGGAAGCCACCATCGCGCACGGCCTGCTGCACCGGGACGTGGAGCAGTGGCTGGTGGCCGATTCTG
CTCGCGCCCGGTTCTACGAAGAGATCTCGCGCAAGACCCGCTTGCGGTCCAAGGACAGCCGCGGCGCGTACTGGTGTGCC
GCCGACGCGTTGGCGATGGCCTTTGCCCTGCACCCGGAAGGCGCGCAGCGGCTGGAACAGCGCCCGGTACACATCGAACT
ATCCGGCACGCATACCCGCGGTATGACCCTGGTGGACTGGAACCGGCAGGGCGGGGCGTCCGACAACGCCAACCTGCTGT
TGGCCTATGACATCCAACAGTTCTACGGCCTGGTTGGAGCGGCCTTGGCGGCGAACTGA

Upstream 100 bases:

>100_bases
ACCAAAGGCCGCTGCGTCCTGCGCGTCCTGGACGTTCGCGTGATCGGCCGATCCTTGCGAAGATGCGGCAACGAACCCCG
AATGCCCAATCCCGAATCCA

Downstream 100 bases:

>100_bases
GGTGACCTTGCGCTGGCTGTCCTGGCGAAGCTATAATCCCCGGCTTATCCAGGCAGCCCGGCGCCAAGTCCATGAAAGAT
AACGTGCATCCCAACTACAA

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTDKIPLLIDTDPGVDDALALLMAFNDARHEVVGLTIAAGNVGLEHTVRNALKVCEIAGRADVPVYAGCSQPLLHPSVDA
AHVHGLDGFGDVGLPPAKRTAEAEHAALAILRLSHQHAGKLLLVALGPLTNLALALILDPTLPTRVARLVVMGGALTGHG
NITAAAEFNIGFDPEAAHIVFRGFPQFDVADWEATIAHGLLHRDVEQWLVADSARARFYEEISRKTRLRSKDSRGAYWCA
ADALAMAFALHPEGAQRLEQRPVHIELSGTHTRGMTLVDWNRQGGASDNANLLLAYDIQQFYGLVGAALAAN

Sequences:

>Translated_312_residues
MTDKIPLLIDTDPGVDDALALLMAFNDARHEVVGLTIAAGNVGLEHTVRNALKVCEIAGRADVPVYAGCSQPLLHPSVDA
AHVHGLDGFGDVGLPPAKRTAEAEHAALAILRLSHQHAGKLLLVALGPLTNLALALILDPTLPTRVARLVVMGGALTGHG
NITAAAEFNIGFDPEAAHIVFRGFPQFDVADWEATIAHGLLHRDVEQWLVADSARARFYEEISRKTRLRSKDSRGAYWCA
ADALAMAFALHPEGAQRLEQRPVHIELSGTHTRGMTLVDWNRQGGASDNANLLLAYDIQQFYGLVGAALAAN
>Mature_311_residues
TDKIPLLIDTDPGVDDALALLMAFNDARHEVVGLTIAAGNVGLEHTVRNALKVCEIAGRADVPVYAGCSQPLLHPSVDAA
HVHGLDGFGDVGLPPAKRTAEAEHAALAILRLSHQHAGKLLLVALGPLTNLALALILDPTLPTRVARLVVMGGALTGHGN
ITAAAEFNIGFDPEAAHIVFRGFPQFDVADWEATIAHGLLHRDVEQWLVADSARARFYEEISRKTRLRSKDSRGAYWCAA
DALAMAFALHPEGAQRLEQRPVHIELSGTHTRGMTLVDWNRQGGASDNANLLLAYDIQQFYGLVGAALAAN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=307, Percent_Identity=33.8762214983713, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=31.25, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786213, Length=314, Percent_Identity=33.1210191082803, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17565698, Length=302, Percent_Identity=30.794701986755, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32565474, Length=290, Percent_Identity=31.3793103448276, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320608, Length=215, Percent_Identity=32.5581395348837, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641839, Length=290, Percent_Identity=32.7586206896552, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24583915, Length=292, Percent_Identity=31.5068493150685, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24641837, Length=319, Percent_Identity=30.4075235109718, Blast_Score=107, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33374; Mature: 33243

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKIPLLIDTDPGVDDALALLMAFNDARHEVVGLTIAAGNVGLEHTVRNALKVCEIAGR
CCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCC
ADVPVYAGCSQPLLHPSVDAAHVHGLDGFGDVGLPPAKRTAEAEHAALAILRLSHQHAGK
CCCCEEECCCCCCCCCCCCHHEECCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHCCCC
LLLVALGPLTNLALALILDPTLPTRVARLVVMGGALTGHGNITAAAEFNIGFDPEAAHIV
EEEEEECCHHHHEEEEEECCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCEEEE
FRGFPQFDVADWEATIAHGLLHRDVEQWLVADSARARFYEEISRKTRLRSKDSRGAYWCA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEH
ADALAMAFALHPEGAQRLEQRPVHIELSGTHTRGMTLVDWNRQGGASDNANLLLAYDIQQ
HHHHHHHHEECCCHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEEHHH
FYGLVGAALAAN
HHHHHHHHHHCC
>Mature Secondary Structure 
TDKIPLLIDTDPGVDDALALLMAFNDARHEVVGLTIAAGNVGLEHTVRNALKVCEIAGR
CCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCC
ADVPVYAGCSQPLLHPSVDAAHVHGLDGFGDVGLPPAKRTAEAEHAALAILRLSHQHAGK
CCCCEEECCCCCCCCCCCCHHEECCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHCCCC
LLLVALGPLTNLALALILDPTLPTRVARLVVMGGALTGHGNITAAAEFNIGFDPEAAHIV
EEEEEECCHHHHEEEEEECCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCEEEE
FRGFPQFDVADWEATIAHGLLHRDVEQWLVADSARARFYEEISRKTRLRSKDSRGAYWCA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEH
ADALAMAFALHPEGAQRLEQRPVHIELSGTHTRGMTLVDWNRQGGASDNANLLLAYDIQQ
HHHHHHHHEECCCHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEEHHH
FYGLVGAALAAN
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA