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The map label for this gene is insH10 [H]

Identifier: 84621917

GI number: 84621917

Start: 283799

End: 284767

Strand: Direct

Name: insH10 [H]

Synonym: XOO_0260

Alternate gene names: 84621917

Gene position: 283799-284767 (Clockwise)

Preceding gene: 84621916

Following gene: 84621918

Centisome position: 5.74

GC content: 61.4

Gene sequence:

>969_bases
ATGCAACTGACGTTCGGTGACGCCGAGGGCCTGGGCAAGCGCAAGCAGACCCGGCGCGAGATCTTCCTTGCGGAGATGGA
GCGCATCGTGCCGTGGAAGCGACTGCTTGCCCTGATCGAGCCGCACTATCCGGTGTCAGGACGACCGGGTCGGCAGCCGT
ACGCGCTGGCGAGGATGTTGCGGATTCATCTGTTGCAGCAGTGGTATGCGTTGAGCGATCCGGCGATGGAAGAGGCATTG
CACGAGATCCCGACCCTGCGGCGTTTTGCCCAGCTCGGCGGCTTGGATAACGTTCCAGACGAGACCACGATTCTCAACTT
TCGCCGTTTGCTGGAAACCCACGCCATTGCCGCTCGGATGCTGGAAGCGGTCAACGCCCATTTGTCGCGCAAGGGGCAGA
GCCTGCGGTCGGGCACGATCGTCGATGCGACGCTGATCGCTGCGCCCAGTTCGACCAAGAATGCCGATCGTGCGCGCGAC
CCTGAGATGCATCAGACCAAGAAGGGCAACCAGTGGTATTTCGGGATGAAGGCGCACATTGGGGTGGATGAATTTTCCGG
GCTGGTACACCACGTGCAGTGCACCGCAGCCAACGTGGCCGATGTCACGGTGACGCACGCATTGCTGCACGGCAAGGAAG
ACAGCGTGTTCGGCGACAGCGGCTACACCGGTGCGGAAAAACGCGACGAGTTGCAGAGCTGCGAGGCTGCATTTTTCATT
GCCGCCAAGCGCTCCACGATTCAAGCCATTGGCAACAAGCGCGCGCGTGCTTGGGCAGAACGTTGGGAACACTTCAAGGC
AAGCGTGCGCGCGAAGGTGGAGCACCCATTCCGGGTGATCAAGCGGCAGTTCGGCTACACCAAGGTGCGCTATCGCGGCC
TGGCCAAGAACACCGCACAGGTGCAGACGTTATTTGCGCTGTCGAATCTGTGGATGGTGCGCCGGCACTTGCTGCCGGCC
AGGGGATAA

Upstream 100 bases:

>100_bases
CGTTGTTTGAGCCGCTCTAAGCCGCAAGCAGCACCTAAGGAACCTCTGAACAACGCACCACAAATGCGAGACACTATTTG
TTCGGAATGAGGAGGCATCC

Downstream 100 bases:

>100_bases
TGCTGCCTGGCGGCAGCCAAAACCGCCAGAACGTTGCAAAAATCGCACCCGACTCAGCATTTTTCCAGTCATTGAAATGC
AAGAAGCTGGAATTTTAGAG

Product: ISXo1 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQLTFGDAEGLGKRKQTRREIFLAEMERIVPWKRLLALIEPHYPVSGRPGRQPYALARMLRIHLLQQWYALSDPAMEEAL
HEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHAIAARMLEAVNAHLSRKGQSLRSGTIVDATLIAAPSSTKNADRARD
PEMHQTKKGNQWYFGMKAHIGVDEFSGLVHHVQCTAANVADVTVTHALLHGKEDSVFGDSGYTGAEKRDELQSCEAAFFI
AAKRSTIQAIGNKRARAWAERWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQVQTLFALSNLWMVRRHLLPA
RG

Sequences:

>Translated_322_residues
MQLTFGDAEGLGKRKQTRREIFLAEMERIVPWKRLLALIEPHYPVSGRPGRQPYALARMLRIHLLQQWYALSDPAMEEAL
HEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHAIAARMLEAVNAHLSRKGQSLRSGTIVDATLIAAPSSTKNADRARD
PEMHQTKKGNQWYFGMKAHIGVDEFSGLVHHVQCTAANVADVTVTHALLHGKEDSVFGDSGYTGAEKRDELQSCEAAFFI
AAKRSTIQAIGNKRARAWAERWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQVQTLFALSNLWMVRRHLLPA
RG
>Mature_322_residues
MQLTFGDAEGLGKRKQTRREIFLAEMERIVPWKRLLALIEPHYPVSGRPGRQPYALARMLRIHLLQQWYALSDPAMEEAL
HEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHAIAARMLEAVNAHLSRKGQSLRSGTIVDATLIAAPSSTKNADRARD
PEMHQTKKGNQWYFGMKAHIGVDEFSGLVHHVQCTAANVADVTVTHALLHGKEDSVFGDSGYTGAEKRDELQSCEAAFFI
AAKRSTIQAIGNKRARAWAERWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQVQTLFALSNLWMVRRHLLPA
RG

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=313, Percent_Identity=53.6741214057508, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI1788303, Length=311, Percent_Identity=53.3762057877814, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1789921, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789612, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789355, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1788518, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1788342, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1787592, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1786877, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1786764, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1786453, Length=311, Percent_Identity=53.0546623794212, Blast_Score=336, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 36464; Mature: 36464

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTFGDAEGLGKRKQTRREIFLAEMERIVPWKRLLALIEPHYPVSGRPGRQPYALARML
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RIHLLQQWYALSDPAMEEALHEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHAIAARM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEAVNAHLSRKGQSLRSGTIVDATLIAAPSSTKNADRARDPEMHQTKKGNQWYFGMKAHI
HHHHHHHHHHCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEC
GVDEFSGLVHHVQCTAANVADVTVTHALLHGKEDSVFGDSGYTGAEKRDELQSCEAAFFI
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AAKRSTIQAIGNKRARAWAERWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
VQTLFALSNLWMVRRHLLPARG
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQLTFGDAEGLGKRKQTRREIFLAEMERIVPWKRLLALIEPHYPVSGRPGRQPYALARML
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RIHLLQQWYALSDPAMEEALHEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHAIAARM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEAVNAHLSRKGQSLRSGTIVDATLIAAPSSTKNADRARDPEMHQTKKGNQWYFGMKAHI
HHHHHHHHHHCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEC
GVDEFSGLVHHVQCTAANVADVTVTHALLHGKEDSVFGDSGYTGAEKRDELQSCEAAFFI
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AAKRSTIQAIGNKRARAWAERWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
VQTLFALSNLWMVRRHLLPARG
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]