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The map label for this gene is pyrF [H]

Identifier: 84621883

GI number: 84621883

Start: 246735

End: 247466

Strand: Direct

Name: pyrF [H]

Synonym: XOO_0226

Alternate gene names: 84621883

Gene position: 246735-247466 (Clockwise)

Preceding gene: 84621882

Following gene: 84621888

Centisome position: 4.99

GC content: 70.77

Gene sequence:

>732_bases
ATGAGCCGGGCGCCGCTGCCGCTGGCCGCCCATGAGCGGCTGATCTTCGCGCTGGATGTGCCCAGCCACGATGAGGCGAT
CGCCTGGGTGGACCGGCTTGGCGATTCGGTGGCGTTCTACAAGATCGGCATGGAACTGCTGGCCTCCGGCGAGTACTTCC
ATGTGCTCGATGCGCTGGCCAAGCGCGACAAGCGCGTGTTCGTGGATCTTAAATTCTTCGACATTCCCGCCACCGTGGCC
GGCACCATCCGTCGCCTGGCACAGTGGCCGGTGAGCTATTGCACCGTGCACGGCTGGCACGCCGGCATGCTGCAGGCTGC
CGCCGACGCCAACCACGGCGCCATGCGTCTGCTGGCGGTGACCGTGCTGACCTCGATGGGCCGCCCGGACCTGGCGGCGA
TGGGCATCGACCGCGAACCGGTGGACGTGGTGGTGGAGCGCGCGCTGGCCGCAGAGGCCGCCGGCATCGATGGCGTCATC
GCCTCCGGCCAGGAAGCCGGCCCGATCCGCCGCGCCACCGGCCCGGCCTTTTCGATCGTCTGCCCCGGCATCCGCCCCGG
AGGCCCGGTCGCCGACGATCAGCAACGCATAGTGGGCGTGGCGCAGGCCTTCACCGACGGCGCCGATGCCATCGTCGTCG
GCCGCCCGATCCGCCTGGCCGCCGATCCGGCCGCGGCTGCGGCGGCGATCCAGGCAGAGATCCTTGCGGCGGTGGGGCAG
GACCGCAGCTGA

Upstream 100 bases:

>100_bases
CCAGCTACGGCGGCTGGGAGCCGGCGCAGTTCAACAACGACTATGCGCAACCCTGGCAAACGCGCCACGATGCCAAGCGC
GCGGCGCTGGAGGTGGCCCG

Downstream 100 bases:

>100_bases
CCGGGCAGCGAAGCTCCGTACGCGCCCTCTGCTAAACAAAATCAATGAGTTAATTAAATATAATTAAAGTGTTGCTTTAA
CGTTAAAGGCTGCGTACGAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSRAPLPLAAHERLIFALDVPSHDEAIAWVDRLGDSVAFYKIGMELLASGEYFHVLDALAKRDKRVFVDLKFFDIPATVA
GTIRRLAQWPVSYCTVHGWHAGMLQAAADANHGAMRLLAVTVLTSMGRPDLAAMGIDREPVDVVVERALAAEAAGIDGVI
ASGQEAGPIRRATGPAFSIVCPGIRPGGPVADDQQRIVGVAQAFTDGADAIVVGRPIRLAADPAAAAAAIQAEILAAVGQ
DRS

Sequences:

>Translated_243_residues
MSRAPLPLAAHERLIFALDVPSHDEAIAWVDRLGDSVAFYKIGMELLASGEYFHVLDALAKRDKRVFVDLKFFDIPATVA
GTIRRLAQWPVSYCTVHGWHAGMLQAAADANHGAMRLLAVTVLTSMGRPDLAAMGIDREPVDVVVERALAAEAAGIDGVI
ASGQEAGPIRRATGPAFSIVCPGIRPGGPVADDQQRIVGVAQAFTDGADAIVVGRPIRLAADPAAAAAAIQAEILAAVGQ
DRS
>Mature_242_residues
SRAPLPLAAHERLIFALDVPSHDEAIAWVDRLGDSVAFYKIGMELLASGEYFHVLDALAKRDKRVFVDLKFFDIPATVAG
TIRRLAQWPVSYCTVHGWHAGMLQAAADANHGAMRLLAVTVLTSMGRPDLAAMGIDREPVDVVVERALAAEAAGIDGVIA
SGQEAGPIRRATGPAFSIVCPGIRPGGPVADDQQRIVGVAQAFTDGADAIVVGRPIRLAADPAAAAAAIQAEILAAVGQD
RS

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=227, Percent_Identity=37.8854625550661, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25503; Mature: 25371

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAPLPLAAHERLIFALDVPSHDEAIAWVDRLGDSVAFYKIGMELLASGEYFHVLDALA
CCCCCCCHHCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KRDKRVFVDLKFFDIPATVAGTIRRLAQWPVSYCTVHGWHAGMLQAAADANHGAMRLLAV
HCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHCCCCCHHHHHHHH
TVLTSMGRPDLAAMGIDREPVDVVVERALAAEAAGIDGVIASGQEAGPIRRATGPAFSIV
HHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEE
CPGIRPGGPVADDQQRIVGVAQAFTDGADAIVVGRPIRLAADPAAAAAAIQAEILAAVGQ
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHCC
DRS
CCC
>Mature Secondary Structure 
SRAPLPLAAHERLIFALDVPSHDEAIAWVDRLGDSVAFYKIGMELLASGEYFHVLDALA
CCCCCCHHCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KRDKRVFVDLKFFDIPATVAGTIRRLAQWPVSYCTVHGWHAGMLQAAADANHGAMRLLAV
HCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHCCCCCHHHHHHHH
TVLTSMGRPDLAAMGIDREPVDVVVERALAAEAAGIDGVIASGQEAGPIRRATGPAFSIV
HHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEE
CPGIRPGGPVADDQQRIVGVAQAFTDGADAIVVGRPIRLAADPAAAAAAIQAEILAAVGQ
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHCC
DRS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]