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The map label for this gene is gltD [H]

Identifier: 84621815

GI number: 84621815

Start: 167631

End: 169112

Strand: Direct

Name: gltD [H]

Synonym: XOO_0158

Alternate gene names: 84621815

Gene position: 167631-169112 (Clockwise)

Preceding gene: 84621814

Following gene: 84621816

Centisome position: 3.39

GC content: 68.02

Gene sequence:

>1482_bases
ATGAGCCGCAAACAAGCCTTCCAATTCCTCGACCTGCCGCGGACCATGCCCACGCGCATTCCGGTGGAACTGCGCACGTC
CGGCGATTGGGGCGAGTTGTACGGCAAGTTCGACAAGGCCGAGGCGCAGTACCAGTCCGGCCGTTGCCTGGATTGCGGCA
ACCCGTATTGCAGCTGGAAATGCCCGGTGCACAACGCCATCCCGCAGTGGCTGCAGCTGGTGCAGGAAAACCGCATCGAG
GAAGCCGCTGCGCTGTGCCATTCCACCAACCCGCTGCCGGAAGTGTGCGGGCGCGTGTGTCCGCAGGACCGCCTGTGCGA
AGGCAGCTGCACGCTGGAAGAATTCGGCGCGGTCACCATCGGTGCGGTAGAGAAATACATCGTCGACACCGCCTTCAAGA
TGGGCTGGCGTCCGGACCTGGGCAACGTGCACCCGACCGGCCACCGCGTGGCGGTGATCGGCGCCGGCCCGGCCGGCCTG
GCGTGTGCGGACAGGCTGGCGCGCGCCGGCATCGAAGCGGTGGTGTACGACCGCTATGAACAAATCGGCGGGCTGCTGCA
GTTCGGCATCCCCAGCTTCAAGCTGGACAAGTCGGTGATCGGCAAGCGCCGCGAGATCCTCGAAGGCATGGGCGTGCAGT
TCCGCCTGGGCGTGGAGATCGGCCGCGACATCAGCATCGAGCAGTTGCTGGGCGAATACGATGCGGTGTTCCTGGGCACC
GGTGCCTACCGCTACACCGACGGCGGCCTGCCCGGGCAGGATCTGAAGAACGTGCTGCCGGCGCTGCCATTCCTGGTGCA
GAACAGCCGCATCGTCAGCGGCAACGACCCGCACGGCCGGCCGATCGCCGGCTGGGAAAACCAGCTCGCCCTGCCCGACC
TCACCGGCAAGCGCGTGGTTGTACTTGGCGGCGGCGACACCGGCATGGACTGCGTGCGCAGCGCCATCCGCCTGGGCGCG
GCCAAGGTCACCTGTGCCTATCGCCGCGACGAAGCCAGCATGCCCGGCTCCGCACGCGAGGTGGCCAACGCCCGCGAAGA
AGGCGTGCGCTTCCTGTTCAACCGGCAGCCGCTGTCGATCGAATCCGGCGCCGACGACGAAGCCATCGGCGTGACCGTGG
TGGAAACCAAGCTGGGTGAGCCCGACGCCAGCGGCCGCCGCAATGCGGTGCCGGTGGAAGGCAGCGAATCGCTGCTGGAA
GCGGACGTGGTGATCATCGCCTTCGGCTTCTCGCCGAGCCTGCCCGACTGGTTGGCGTCGCAAGGCGTGGAAGCCGGCAG
CAACGGCCGCATCGTCGCCCCGGCCGACGGCCACGGCCGCCTGCCCTACCAGACCAGCAACCCGCGCCTGTTCGCAGGCG
GCGACTGCGTGCGCGGCGCCGATTTGGTGGTGACTGCAGTGGCCGAGGGCCGCGACGCAGCCAGCAGCATCGTGCAACTG
CTGGGTGTGAAAGCGCAGGTCAAGGAGCCGGCTGCGGCGTAG

Upstream 100 bases:

>100_bases
TCCTCCAGCGTCGTGATTGTTCATTCGAGATTCGCACTGCGTCCGCTCCCCGTCGCCCGCTGTTGCGAATCCCCAATCCC
ACCCCCCGAATCCCAGCCCT

Downstream 100 bases:

>100_bases
GGACGTTAGCCCCATGCTCCCCTGCACCACGTGCAGGTCTCCCGCTATGCACATGCCGTTCACACAGTTCGTTCTACAGC
CCCCCACGCGAACCGAACAC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKAEAQYQSGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIE
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVHPTGHRVAVIGAGPAGL
ACADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGRDISIEQLLGEYDAVFLGT
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWENQLALPDLTGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPSLPDWLASQGVEAGSNGRIVAPADGHGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAASSIVQL
LGVKAQVKEPAAA

Sequences:

>Translated_493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKAEAQYQSGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIE
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVHPTGHRVAVIGAGPAGL
ACADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGRDISIEQLLGEYDAVFLGT
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWENQLALPDLTGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPSLPDWLASQGVEAGSNGRIVAPADGHGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAASSIVQL
LGVKAQVKEPAAA
>Mature_492_residues
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKAEAQYQSGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIEE
AAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVHPTGHRVAVIGAGPAGLA
CADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGRDISIEQLLGEYDAVFLGTG
AYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWENQLALPDLTGKRVVVLGGGDTGMDCVRSAIRLGAA
KVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEA
DVVIIAFGFSPSLPDWLASQGVEAGSNGRIVAPADGHGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAASSIVQLL
GVKAQVKEPAAA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=29.0748898678414, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=57.6763485477178, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=54.2797494780793, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=53.9301310043668, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1788468, Length=451, Percent_Identity=29.0465631929047, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=32.3595505617978, Blast_Score=158, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17570289, Length=491, Percent_Identity=33.8085539714868, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71984108, Length=452, Percent_Identity=30.3097345132743, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17543860, Length=172, Percent_Identity=31.3953488372093, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=476, Percent_Identity=35.5042016806723, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI28574881, Length=479, Percent_Identity=36.5344467640919, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24665539, Length=479, Percent_Identity=36.5344467640919, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24665547, Length=479, Percent_Identity=36.5344467640919, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24665543, Length=479, Percent_Identity=36.5344467640919, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24640763, Length=458, Percent_Identity=30.5676855895196, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI18858217, Length=458, Percent_Identity=30.5676855895196, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52799; Mature: 52667

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKAEAQYQSGRCLDCGNPYCSWK
CCHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCHHHHHHHHCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQENRIEEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFKMGWRPDLGNVHPTGHRVAVIGAGPAGLACADRLARAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGRDISIEQLLGEYDAVFLGT
HHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWENQLALPDLTGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPSLPDWLAS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVEAGSNGRIVAPADGHGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAASSIVQL
CCCCCCCCCCEEECCCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHH
LGVKAQVKEPAAA
HCCHHHCCCCCCC
>Mature Secondary Structure 
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKAEAQYQSGRCLDCGNPYCSWK
CHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCHHHHHHHHCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQENRIEEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFKMGWRPDLGNVHPTGHRVAVIGAGPAGLACADRLARAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGRDISIEQLLGEYDAVFLGT
HHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWENQLALPDLTGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPSLPDWLAS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVEAGSNGRIVAPADGHGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAASSIVQL
CCCCCCCCCCEEECCCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHH
LGVKAQVKEPAAA
HCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]