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The map label for this gene is macA [H]

Identifier: 84621791

GI number: 84621791

Start: 136224

End: 137471

Strand: Direct

Name: macA [H]

Synonym: XOO_0134

Alternate gene names: 84621791

Gene position: 136224-137471 (Clockwise)

Preceding gene: 84621790

Following gene: 84621792

Centisome position: 2.76

GC content: 66.43

Gene sequence:

>1248_bases
ATGAACAACTCTGCCGATCTGCTGAAGGAACTGCGCATCGACCGTAAGGCCCCGTCCCGCGAATCGCCCTCGGGGCGCGG
ATGGGGGATCGCCATCGCCACGCTCGTCGTCGTTATCGTGCTGCTGCTCGGTTTAGCCGCGTGGTGGTGGTGGCTTGGTC
GGGCGCGGCCGCTGCAGGTGCGCACCGTACCGGTGGTGGCGATCAGCGCCGGCAGCACGTCGTCGTCGGTCCTTGATGCC
AGCGGCTACGTGGTGGCGCGACGCATAGCCACGGTGTCGGCGCAGGTCACCGGCAAGGTGCTGCAGGTGATGATCGAGGA
AGGCATGCGCGTGGAGGCCGGTCAGGTGATGGCCACGCTCGATCCGATCGAGACCAATGCGCAGCGCGAGGTCGGTGCCG
CGCAACTCGCGGCGGCGCGCAGTCAGGTGCAGAACGTCCAGGCGCAGCTGGTCGTGGCCGAGGCCGATGCGATGCGGCTG
CAGTCGCTGGTCGGCGAGCAACTGGTATCGCGTTCGCAGTACGATCAGGCGATGTCGCAGCGCGATGCGCTGCGCGCGCA
GCTGCAGGTGGCCCAGCGCAATGTCGCGGTCGCCACGCACGAGTTGTCGCTGTCAGACATCCACGTCGACTTCACCGTGG
TGCGCGCGCCGTTCGCCGGCGTGGTCACTGCCAAGGCCGCGCAGCCGGGCGAGATCGTCTCGCCGCTGGCCACCGGCGGT
TTCACCCGCACCGGCATGATTGTCGACATGGATTCGTTGGAAGTGGAAGTGGAGGTTGGCGAGGCCTACATCGGCCGCGT
GCAGCCGAAGATGCCGGCCGAAGTGATCCTCGACGCCTATCCAGACTGGCGCATCCCGGCCAAGGTGATCGCGATCATCC
CGGCTGCCGACCGCGGCAAGGCCACAGTCAAGGTGCGCGTGGCGCTTAAGGTCAAGGATGCACGCATCGTGCCGGACATG
GGCGTGCGGGTGAGTTTTTTGGAAAAGGCCACGGCGCAGCAGCAGAACACGCCGAAGGGCGTGCGGGTACCGGTTGCGTC
ACTGCTGCATCGTGACGGCACCAGCCGCGTCTTCGTCCTCGGCAACGACAACCACGTCGCCCTGCGCGAGGTGAAGACCG
GCATCGACATGGGCAGCGACAAGCAGGTGGTATCAGGCGTATCTGCGGGCGATACAGTGGTCGTCGAGCCACCGCAGGCG
TTGAAGGATGGAGATGCGGTGACGTTGTCCAGCGACGCACAGGAGTGA

Upstream 100 bases:

>100_bases
CCCGACGATACGCATCATGATGCTGTAGAGTCGCGTCCCAGGATTCGCCTCCAACTTAATGACTTCCTAGAGCGTGACAC
GATCACGTTAACAGGTCTCA

Downstream 100 bases:

>100_bases
GCGCGCTGCTGCGCGCGCCGGTGTGGTCTTCGTTTGATGTTGCGTGGATTGGTTCGAGGTCGTGAGCGGCGTCGGCAGTC
GACCGAGGAAGGCGCTGCAC

Product: acriflavin resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNNSADLLKELRIDRKAPSRESPSGRGWGIAIATLVVVIVLLLGLAAWWWWLGRARPLQVRTVPVVAISAGSTSSSVLDA
SGYVVARRIATVSAQVTGKVLQVMIEEGMRVEAGQVMATLDPIETNAQREVGAAQLAAARSQVQNVQAQLVVAEADAMRL
QSLVGEQLVSRSQYDQAMSQRDALRAQLQVAQRNVAVATHELSLSDIHVDFTVVRAPFAGVVTAKAAQPGEIVSPLATGG
FTRTGMIVDMDSLEVEVEVGEAYIGRVQPKMPAEVILDAYPDWRIPAKVIAIIPAADRGKATVKVRVALKVKDARIVPDM
GVRVSFLEKATAQQQNTPKGVRVPVASLLHRDGTSRVFVLGNDNHVALREVKTGIDMGSDKQVVSGVSAGDTVVVEPPQA
LKDGDAVTLSSDAQE

Sequences:

>Translated_415_residues
MNNSADLLKELRIDRKAPSRESPSGRGWGIAIATLVVVIVLLLGLAAWWWWLGRARPLQVRTVPVVAISAGSTSSSVLDA
SGYVVARRIATVSAQVTGKVLQVMIEEGMRVEAGQVMATLDPIETNAQREVGAAQLAAARSQVQNVQAQLVVAEADAMRL
QSLVGEQLVSRSQYDQAMSQRDALRAQLQVAQRNVAVATHELSLSDIHVDFTVVRAPFAGVVTAKAAQPGEIVSPLATGG
FTRTGMIVDMDSLEVEVEVGEAYIGRVQPKMPAEVILDAYPDWRIPAKVIAIIPAADRGKATVKVRVALKVKDARIVPDM
GVRVSFLEKATAQQQNTPKGVRVPVASLLHRDGTSRVFVLGNDNHVALREVKTGIDMGSDKQVVSGVSAGDTVVVEPPQA
LKDGDAVTLSSDAQE
>Mature_415_residues
MNNSADLLKELRIDRKAPSRESPSGRGWGIAIATLVVVIVLLLGLAAWWWWLGRARPLQVRTVPVVAISAGSTSSSVLDA
SGYVVARRIATVSAQVTGKVLQVMIEEGMRVEAGQVMATLDPIETNAQREVGAAQLAAARSQVQNVQAQLVVAEADAMRL
QSLVGEQLVSRSQYDQAMSQRDALRAQLQVAQRNVAVATHELSLSDIHVDFTVVRAPFAGVVTAKAAQPGEIVSPLATGG
FTRTGMIVDMDSLEVEVEVGEAYIGRVQPKMPAEVILDAYPDWRIPAKVIAIIPAADRGKATVKVRVALKVKDARIVPDM
GVRVSFLEKATAQQQNTPKGVRVPVASLLHRDGTSRVFVLGNDNHVALREVKTGIDMGSDKQVVSGVSAGDTVVVEPPQA
LKDGDAVTLSSDAQE

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=362, Percent_Identity=24.0331491712707, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=25.8953168044077, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082036, Length=391, Percent_Identity=24.2966751918159, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786668, Length=336, Percent_Identity=25.8928571428571, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789665, Length=350, Percent_Identity=24, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44260; Mature: 44260

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSADLLKELRIDRKAPSRESPSGRGWGIAIATLVVVIVLLLGLAAWWWWLGRARPLQV
CCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
RTVPVVAISAGSTSSSVLDASGYVVARRIATVSAQVTGKVLQVMIEEGMRVEAGQVMATL
EEEEEEEEECCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEE
DPIETNAQREVGAAQLAAARSQVQNVQAQLVVAEADAMRLQSLVGEQLVSRSQYDQAMSQ
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDALRAQLQVAQRNVAVATHELSLSDIHVDFTVVRAPFAGVVTAKAAQPGEIVSPLATGG
HHHHHHHHHHHHCCEEEEEEEEEEEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHCCC
FTRTGMIVDMDSLEVEVEVGEAYIGRVQPKMPAEVILDAYPDWRIPAKVIAIIPAADRGK
CEECCEEEEECCEEEEEEECCHHHCCCCCCCCHHHHEECCCCCCCCEEEEEEEECCCCCC
ATVKVRVALKVKDARIVPDMGVRVSFLEKATAQQQNTPKGVRVPVASLLHRDGTSRVFVL
EEEEEEEEEEEECCEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEE
GNDNHVALREVKTGIDMGSDKQVVSGVSAGDTVVVEPPQALKDGDAVTLSSDAQE
ECCCEEEEEEHHHCCCCCCCHHHHCCCCCCCEEEECCCHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MNNSADLLKELRIDRKAPSRESPSGRGWGIAIATLVVVIVLLLGLAAWWWWLGRARPLQV
CCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
RTVPVVAISAGSTSSSVLDASGYVVARRIATVSAQVTGKVLQVMIEEGMRVEAGQVMATL
EEEEEEEEECCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEE
DPIETNAQREVGAAQLAAARSQVQNVQAQLVVAEADAMRLQSLVGEQLVSRSQYDQAMSQ
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDALRAQLQVAQRNVAVATHELSLSDIHVDFTVVRAPFAGVVTAKAAQPGEIVSPLATGG
HHHHHHHHHHHHCCEEEEEEEEEEEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHCCC
FTRTGMIVDMDSLEVEVEVGEAYIGRVQPKMPAEVILDAYPDWRIPAKVIAIIPAADRGK
CEECCEEEEECCEEEEEEECCHHHCCCCCCCCHHHHEECCCCCCCCEEEEEEEECCCCCC
ATVKVRVALKVKDARIVPDMGVRVSFLEKATAQQQNTPKGVRVPVASLLHRDGTSRVFVL
EEEEEEEEEEEECCEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEE
GNDNHVALREVKTGIDMGSDKQVVSGVSAGDTVVVEPPQALKDGDAVTLSSDAQE
ECCCEEEEEEHHHCCCCCCCHHHHCCCCCCCEEEECCCHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]