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The map label for this gene is hisH

Identifier: 84490290

GI number: 84490290

Start: 1693487

End: 1693945

Strand: Direct

Name: hisH

Synonym: Msp_1510

Alternate gene names: 84490290

Gene position: 1693487-1693945 (Clockwise)

Preceding gene: 84490289

Following gene: 84490291

Centisome position: 95.82

GC content: 29.41

Gene sequence:

>459_bases
ATGAAGAATCTTGTTAAGTATAAGGACGTTGTATATAATCATATAGATGATGATAAACCTTTACTAGGAATCTGTTTAGG
ATTACATATGCTTTTTTCTAGTAGTCAGGAGAGTCCTGATATTAAGGGGTTGGATATATTTAATGGTAGTGCAAAGAAAT
TTAACTTACCTCCAGAATATAAAATACCTCATATGGGATGGAATACTATAGAAGTAAATGATACTACTTCAAATAACACT
ACTCTTCTATCTGAAGCTGACAATAACTATATGTACTTTGTTCATTCATATTATATTGACCCTGAGGATAAAAATATAAT
AACTGCATATACTGATTATGGTGGAAAAATTCCAGTTGCTATTGGAAAGAATAATATTCATGCACTACAATTCCATCCAG
AAAAAAGTGGAAAAGCAGGATTAAATATTCTACGTAAATTTATAAACACCATAGATTAA

Upstream 100 bases:

>100_bases
AAAACGTTTAAATGTTGAAGTAAAAGTATCTTCTGATATAAATGAAATTAAAACTTCTGATGCATTAATATTACCAGGAG
TAGGTGCATTTGGTGCAGTT

Downstream 100 bases:

>100_bases
CCACCTTTTCTTCTTATTTTTTATAATATTAGAAATTAATATAATAATAACAAAGTATGATATATAGATATAATCATACA
AATTTTATGAAAATTAATAA

Product: hypothetical protein

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKNLVKYKDVVYNHIDDDKPLLGICLGLHMLFSSSQESPDIKGLDIFNGSAKKFNLPPEYKIPHMGWNTIEVNDTTSNNT
TLLSEADNNYMYFVHSYYIDPEDKNIITAYTDYGGKIPVAIGKNNIHALQFHPEKSGKAGLNILRKFINTID

Sequences:

>Translated_152_residues
MKNLVKYKDVVYNHIDDDKPLLGICLGLHMLFSSSQESPDIKGLDIFNGSAKKFNLPPEYKIPHMGWNTIEVNDTTSNNT
TLLSEADNNYMYFVHSYYIDPEDKNIITAYTDYGGKIPVAIGKNNIHALQFHPEKSGKAGLNILRKFINTID
>Mature_152_residues
MKNLVKYKDVVYNHIDDDKPLLGICLGLHMLFSSSQESPDIKGLDIFNGSAKKFNLPPEYKIPHMGWNTIEVNDTTSNNT
TLLSEADNNYMYFVHSYYIDPEDKNIITAYTDYGGKIPVAIGKNNIHALQFHPEKSGKAGLNILRKFINTID

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=133, Percent_Identity=37.593984962406, Blast_Score=88, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=151, Percent_Identity=36.4238410596026, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 17211; Mature: 17211

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLVKYKDVVYNHIDDDKPLLGICLGLHMLFSSSQESPDIKGLDIFNGSAKKFNLPPEY
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCC
KIPHMGWNTIEVNDTTSNNTTLLSEADNNYMYFVHSYYIDPEDKNIITAYTDYGGKIPVA
CCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEECCCCCEEEEEEECCCCEEEEE
IGKNNIHALQFHPEKSGKAGLNILRKFINTID
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLVKYKDVVYNHIDDDKPLLGICLGLHMLFSSSQESPDIKGLDIFNGSAKKFNLPPEY
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCC
KIPHMGWNTIEVNDTTSNNTTLLSEADNNYMYFVHSYYIDPEDKNIITAYTDYGGKIPVA
CCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEECCCCCEEEEEEECCCCEEEEE
IGKNNIHALQFHPEKSGKAGLNILRKFINTID
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]