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The map label for this gene is cofE

Identifier: 84490184

GI number: 84490184

Start: 1577568

End: 1578332

Strand: Direct

Name: cofE

Synonym: Msp_1402

Alternate gene names: 84490184

Gene position: 1577568-1578332 (Clockwise)

Preceding gene: 84490183

Following gene: 84490185

Centisome position: 89.26

GC content: 32.68

Gene sequence:

>765_bases
ATGAGTTTAGAAATTATAGGTGTGGAGAATTTTCCAATTATTGAAGAAAATGATGATTTAGCTAGAATAATATGTGATAC
ACTTAATTATAATAATATTAAGTTAGAAGATGATGATGTTTTAGTTCTTGCAGAGACTGTTGTATCTAAAGCTGAAGGAA
ATTATGTTAAGATTGAGGATGTTGTTGTTTCAGATAAAGCAAAAGACATTTCTTTAAAATCAGGAAAAGATGCTAAACAA
TGTCAAATTATTCTTGATAATACTAAAAAAATTATTAGATGTCAAACTGGACTTATTATAACAGAAACCCCTCATGGTTT
TATCTGTGCTAATTCTGGAATTGATAATAGTAACTGTAGAGAAGGTTATGTTACACCCCTTCCAGTGGATGCTGATAGAA
GTGCAAGATGTATAAAAAAATATCTTGATGAAGTCTTTGATGTGGATGTTAGTGTTATTATCTCTGATACACAGGGCAGA
CCATGGCGTGTTGGTGCTGTTGGTGTTGCTATTGGTATCAGTGGTATTCATCCTTGTACTGATTTTAGAGGAAGTAGCGA
TTTGTATGGTCAAGAATTGATGAGTACAATTGAAGGTACTGCTGATGAACTTGCTAGTGCTGCATCCTTAGTTATGGGTC
AATCTGATAATGGTATTTGTCTTGTTTTAATTAGAGGTTATGATAATTCCTTAGTAAAATGCGCTATTGATGAGGCAAGT
ATTATTGAATCTATTCGCGATGAAGAAACTGATGTGTTTAGGTAG

Upstream 100 bases:

>100_bases
CTTGTTTTATTTAAAAATTTTATATTAACAATTCAATATATATTATAAATAATTAAGATTATGGTCTGGCTTTAAATAGA
TTAAAAGAAGATGATTTATT

Downstream 100 bases:

>100_bases
TGTTACTATGATTACTGTTTTATCTGGCGGAACTGGTACTCCTAAATTATTACAAGGTATTAAAGAAATTATTAAAGCCA
AAGACTTAACTGTTATTGTA

Product: CofE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSLEIIGVENFPIIEENDDLARIICDTLNYNNIKLEDDDVLVLAETVVSKAEGNYVKIEDVVVSDKAKDISLKSGKDAKQ
CQIILDNTKKIIRCQTGLIITETPHGFICANSGIDNSNCREGYVTPLPVDADRSARCIKKYLDEVFDVDVSVIISDTQGR
PWRVGAVGVAIGISGIHPCTDFRGSSDLYGQELMSTIEGTADELASAASLVMGQSDNGICLVLIRGYDNSLVKCAIDEAS
IIESIRDEETDVFR

Sequences:

>Translated_254_residues
MSLEIIGVENFPIIEENDDLARIICDTLNYNNIKLEDDDVLVLAETVVSKAEGNYVKIEDVVVSDKAKDISLKSGKDAKQ
CQIILDNTKKIIRCQTGLIITETPHGFICANSGIDNSNCREGYVTPLPVDADRSARCIKKYLDEVFDVDVSVIISDTQGR
PWRVGAVGVAIGISGIHPCTDFRGSSDLYGQELMSTIEGTADELASAASLVMGQSDNGICLVLIRGYDNSLVKCAIDEAS
IIESIRDEETDVFR
>Mature_253_residues
SLEIIGVENFPIIEENDDLARIICDTLNYNNIKLEDDDVLVLAETVVSKAEGNYVKIEDVVVSDKAKDISLKSGKDAKQC
QIILDNTKKIIRCQTGLIITETPHGFICANSGIDNSNCREGYVTPLPVDADRSARCIKKYLDEVFDVDVSVIISDTQGRP
WRVGAVGVAIGISGIHPCTDFRGSSDLYGQELMSTIEGTADELASAASLVMGQSDNGICLVLIRGYDNSLVKCAIDEASI
IESIRDEETDVFR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27669; Mature: 27537

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEIIGVENFPIIEENDDLARIICDTLNYNNIKLEDDDVLVLAETVVSKAEGNYVKIED
CEEEEEECCCCCEEECCCCHHHHHHHHCCCCEEEECCCCEEEEEHHHHHHCCCCEEEEEE
VVVSDKAKDISLKSGKDAKQCQIILDNTKKIIRCQTGLIITETPHGFICANSGIDNSNCR
EEECCCCCCCCCCCCCCHHHEEEEECCCCEEEEEECCEEEEECCCCEEEECCCCCCCCCC
EGYVTPLPVDADRSARCIKKYLDEVFDVDVSVIISDTQGRPWRVGAVGVAIGISGIHPCT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCC
DFRGSSDLYGQELMSTIEGTADELASAASLVMGQSDNGICLVLIRGYDNSLVKCAIDEAS
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCHHH
IIESIRDEETDVFR
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SLEIIGVENFPIIEENDDLARIICDTLNYNNIKLEDDDVLVLAETVVSKAEGNYVKIED
EEEEEECCCCCEEECCCCHHHHHHHHCCCCEEEECCCCEEEEEHHHHHHCCCCEEEEEE
VVVSDKAKDISLKSGKDAKQCQIILDNTKKIIRCQTGLIITETPHGFICANSGIDNSNCR
EEECCCCCCCCCCCCCCHHHEEEEECCCCEEEEEECCEEEEECCCCEEEECCCCCCCCCC
EGYVTPLPVDADRSARCIKKYLDEVFDVDVSVIISDTQGRPWRVGAVGVAIGISGIHPCT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCC
DFRGSSDLYGQELMSTIEGTADELASAASLVMGQSDNGICLVLIRGYDNSLVKCAIDEAS
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCHHH
IIESIRDEETDVFR
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA