Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 84490074

GI number: 84490074

Start: 1451055

End: 1452293

Strand: Direct

Name: murD [H]

Synonym: Msp_1286

Alternate gene names: 84490074

Gene position: 1451055-1452293 (Clockwise)

Preceding gene: 84490069

Following gene: 84490075

Centisome position: 82.1

GC content: 26.15

Gene sequence:

>1239_bases
ATGAAAGTGAGTGTTGTAGGATTAGGTGTTGAGGGACAAAAAGCCACCATTTCTTTATTAAAACGAGGATATGATGTATA
TTCATCAGATATTAATAGGAACATAAATTTAAGTCTTTTAGAAAAAGAACCCTTAAATACTCAGAAGTTAGATTTAGAAA
TTGGAAGTCATAATCTAGATAAAATATATAAATCAGACCTTGTCTCAGTAAGTCCAAGTTTATTTAAAAAAGAGATTTGT
AAAAATATAATAGAAAGGGAAATCTTTATTTCAGACATACTAACCAAACATAAACAAATTAAAACAATTGCAGTAACTGG
AACTAATGGAAAAACAACAACCACACATATGATTTATCATATCTTAAAAAATGAAGGATATAAAGTGGCTATTGGTGGTA
ATGGTGGTGGTGGTTTTTCAGGATACAATGAATTACTACTTGATACTAATGAAAATGAATATGATTATATGATTATAGAA
GTATGTGATATGACACTTGACTTTTGTAACTATGTGTTTGATATTGACATGGTAGTTGTAACTAATATTGGTTATGATCA
TATGGATGTTCATAAATCTATAGAACATTATACAGAAGAAGTTGGTTGTTTTATAGAAAATAAAGTAGCTATTCTAAATT
ATAATGATAAAAACCTTCAAAAAATTAAAAATGAAAGTAGGGAAACATACTTCTTTAATACATACAAACATGAACTAAAT
TTATTTGGTAAATTTAATCTACAAAATGCAGAAGCAGCACGCATAACATGTAATAAGTTAGGTGTATTAGATGAAAATAT
TATAAATTCCTTAAAAACATTTGAATCTGTTGAAGGTCGAACTATAAAAATCAACTATAATGATAATGAAATAGTTTCTG
GAAAAACTGATAATGTAGATGCCCTAAAAGCTGTTCTTGAAGAAGAAAGATTTGATATTCTAATTATTGGTACACCACGT
AAATATGAAACTTGTAGATATAATATTCTAGATTACATTAACAATTATAAACCTGATACTCTTATTATTTTCCCTGGACT
TGAAGACATAACATATGATTATGTACAATACTTGAATAATATTGGTTATACTAAGGATATGATTGTACTTAAAAATATAG
ATGATATTCTAAACTTTATTAATACTAAAAAAGGATATAAAATATTCGTTGGTGGAAATGGACAGGAAAAAATAACTCAA
ATAACAAACATTCTAAAAGAAGAAAAAACATTCCATTAA

Upstream 100 bases:

>100_bases
ATATAAGAATAATCAAGATTAGATAAAAACTATATAATTCAATTTAATAGAGATAATACAAAACATATGTGAAGAATTAT
ATAAAAGGAGAGTTTTTATA

Downstream 100 bases:

>100_bases
ATATTTTTTTTATAGATTCTTTTTTATAGATAAAACCTTATTTAATTTCAAGTATAAGTAACTAAATTCAAAAGTGATAT
TATGAGTATACAAATAAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKVSVVGLGVEGQKATISLLKRGYDVYSSDINRNINLSLLEKEPLNTQKLDLEIGSHNLDKIYKSDLVSVSPSLFKKEIC
KNIIEREIFISDILTKHKQIKTIAVTGTNGKTTTTHMIYHILKNEGYKVAIGGNGGGGFSGYNELLLDTNENEYDYMIIE
VCDMTLDFCNYVFDIDMVVVTNIGYDHMDVHKSIEHYTEEVGCFIENKVAILNYNDKNLQKIKNESRETYFFNTYKHELN
LFGKFNLQNAEAARITCNKLGVLDENIINSLKTFESVEGRTIKINYNDNEIVSGKTDNVDALKAVLEEERFDILIIGTPR
KYETCRYNILDYINNYKPDTLIIFPGLEDITYDYVQYLNNIGYTKDMIVLKNIDDILNFINTKKGYKIFVGGNGQEKITQ
ITNILKEEKTFH

Sequences:

>Translated_412_residues
MKVSVVGLGVEGQKATISLLKRGYDVYSSDINRNINLSLLEKEPLNTQKLDLEIGSHNLDKIYKSDLVSVSPSLFKKEIC
KNIIEREIFISDILTKHKQIKTIAVTGTNGKTTTTHMIYHILKNEGYKVAIGGNGGGGFSGYNELLLDTNENEYDYMIIE
VCDMTLDFCNYVFDIDMVVVTNIGYDHMDVHKSIEHYTEEVGCFIENKVAILNYNDKNLQKIKNESRETYFFNTYKHELN
LFGKFNLQNAEAARITCNKLGVLDENIINSLKTFESVEGRTIKINYNDNEIVSGKTDNVDALKAVLEEERFDILIIGTPR
KYETCRYNILDYINNYKPDTLIIFPGLEDITYDYVQYLNNIGYTKDMIVLKNIDDILNFINTKKGYKIFVGGNGQEKITQ
ITNILKEEKTFH
>Mature_412_residues
MKVSVVGLGVEGQKATISLLKRGYDVYSSDINRNINLSLLEKEPLNTQKLDLEIGSHNLDKIYKSDLVSVSPSLFKKEIC
KNIIEREIFISDILTKHKQIKTIAVTGTNGKTTTTHMIYHILKNEGYKVAIGGNGGGGFSGYNELLLDTNENEYDYMIIE
VCDMTLDFCNYVFDIDMVVVTNIGYDHMDVHKSIEHYTEEVGCFIENKVAILNYNDKNLQKIKNESRETYFFNTYKHELN
LFGKFNLQNAEAARITCNKLGVLDENIINSLKTFESVEGRTIKINYNDNEIVSGKTDNVDALKAVLEEERFDILIIGTPR
KYETCRYNILDYINNYKPDTLIIFPGLEDITYDYVQYLNNIGYTKDMIVLKNIDDILNFINTKKGYKIFVGGNGQEKITQ
ITNILKEEKTFH

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF01494 FAD_binding_3; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47171; Mature: 47171

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSVVGLGVEGQKATISLLKRGYDVYSSDINRNINLSLLEKEPLNTQKLDLEIGSHNLD
CEEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCHH
KIYKSDLVSVSPSLFKKEICKNIIEREIFISDILTKHKQIKTIAVTGTNGKTTTTHMIYH
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHH
ILKNEGYKVAIGGNGGGGFSGYNELLLDTNENEYDYMIIEVCDMTLDFCNYVFDIDMVVV
HHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHEEEEEEE
TNIGYDHMDVHKSIEHYTEEVGCFIENKVAILNYNDKNLQKIKNESRETYFFNTYKHELN
EECCCCHHHHHHHHHHHHHHHCEEEECCEEEEECCCHHHHHHHCCCCCEEEEEEEEEEEE
LFGKFNLQNAEAARITCNKLGVLDENIINSLKTFESVEGRTIKINYNDNEIVSGKTDNVD
EEEEECCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCCHH
ALKAVLEEERFDILIIGTPRKYETCRYNILDYINNYKPDTLIIFPGLEDITYDYVQYLNN
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
IGYTKDMIVLKNIDDILNFINTKKGYKIFVGGNGQEKITQITNILKEEKTFH
CCCCCCEEEEECHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVSVVGLGVEGQKATISLLKRGYDVYSSDINRNINLSLLEKEPLNTQKLDLEIGSHNLD
CEEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCHH
KIYKSDLVSVSPSLFKKEICKNIIEREIFISDILTKHKQIKTIAVTGTNGKTTTTHMIYH
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHH
ILKNEGYKVAIGGNGGGGFSGYNELLLDTNENEYDYMIIEVCDMTLDFCNYVFDIDMVVV
HHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHEEEEEEE
TNIGYDHMDVHKSIEHYTEEVGCFIENKVAILNYNDKNLQKIKNESRETYFFNTYKHELN
EECCCCHHHHHHHHHHHHHHHCEEEECCEEEEECCCHHHHHHHCCCCCEEEEEEEEEEEE
LFGKFNLQNAEAARITCNKLGVLDENIINSLKTFESVEGRTIKINYNDNEIVSGKTDNVD
EEEEECCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCCHH
ALKAVLEEERFDILIIGTPRKYETCRYNILDYINNYKPDTLIIFPGLEDITYDYVQYLNN
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
IGYTKDMIVLKNIDDILNFINTKKGYKIFVGGNGQEKITQITNILKEEKTFH
CCCCCCEEEEECHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA