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The map label for this gene is lpdA [C]

Identifier: 84489871

GI number: 84489871

Start: 1217024

End: 1218331

Strand: Direct

Name: lpdA [C]

Synonym: Msp_1077

Alternate gene names: 84489871

Gene position: 1217024-1218331 (Clockwise)

Preceding gene: 84489870

Following gene: 84489876

Centisome position: 68.86

GC content: 30.28

Gene sequence:

>1308_bases
ATGACTAGAATTGTTATTATTGGTGCAGGACCTGCTGGACGTTTTGCTTCCATGGCTGCTGCTGAAGTTGGAAATGAAGT
TACTCTTATTGAAAATAAACATATTGGTGGAAAATGTCTTAATCAAGCATGTATGGTTGTATGTGCATTGAATGATGTTT
CAAAACATGTTCTTGATGCAGAAAATTTTAATAATCAGGGAATTATAGATTCCACACCTACTGTTAATTATAAAAGAGTT
ACTACATTAATTAAAGAAACTCAAAAAAAAATACGTAAAATTCTAACTAAAGAAACTATTAATACAGGGGTAGAATATGT
TCAAGGTAGAGCTGAGGTTGATATTGAAAATAAAATGGTTATTGTTAATGAAGATGATGAATATCCATTTGATAAATTAT
TAGTATGTACTGGTTCCTCCCCATATATTCCAGATATTCCTGGAGTTCATAATGCATATACCTATAAGGATACTTTGAAG
ATTAATGAAGTTCCAGAAAAATTAGTTATTATTGGTGGAGGTTCCACTGCTGCTGAATATGCTAATATATTTTCTAGTCT
TGGAAGTCAAGTTGAAATTTTATGCAGAACACAGTTTCTTAAAATGTTAAATGACTCTGAAGCTGAGGAATATATAGTAT
CTAAATTACTTAAAAATACAATCATACATGAAAATGTTGAAATTCAGGAGATTACAGATACATCAGTTGTTACTAATTTT
GGAGAAATTGAAGGTACTGTTTTACTTGCAACTGGTGTAGTTGCCAATTCAGAAATTGTTAAGGATATTGTGGAACTTGA
CAGTGATGGAAACATACTTGTTGATGAATTCATGCAAACTTCCCATGAAGATATTTATGCTGCTGGAGATGTAATTGGTG
GCATATTATCCACACCAGTATCACGTATGGAAGGTATATGTGCTATAAAAAATATTATGGGAAAAAAAGTAAAACCTGAT
TATTCATTAATACCTCTGACAATATCTCTATCATATGATGTAAGTTATATGTTAGGTAATCAAGTATCATCTATTGGAAA
AGAAGTAACTTTACCAGGAGCTGCAGGACCGGGTTCATTTTGGAATACATTAAATGGAAAAACTGGTTTTACAAAAGAAG
TTGTAAATAATGATGGAGATATTACAAATATACTTGCTATTACCCCATCATCAAATGTTGCACTTCCCTATATGATTAAA
TTAATAAAATCAGGAAGTAAAGTTAGTGATTTTGAAGATTTTATTGAAATTCATCCTACAACAGATGGTATTTTTAAATT
AACTGAATATTTTAAACGTTATTTATAA

Upstream 100 bases:

>100_bases
AAAAAAAATATTTTTTATAACAAATATCATGTAATAAAAAGAATATATTATACTATAGATATACTTTTTTATTTAAAATA
AAATTAAAGAAATGATATTT

Downstream 100 bases:

>100_bases
CATTTATCTTTTTTTTAAAAAAAAGAAGAATGAAGATTTATTTCATGTATTTTTCTACAAATTCTACTAAATCCTCAGGA
GCTTTTATTATATCTTTAGC

Product: dihydrolipoamide dehydrogenase-related protein

Products: protein N6-(lipoyl)lysine; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTRIVIIGAGPAGRFASMAAAEVGNEVTLIENKHIGGKCLNQACMVVCALNDVSKHVLDAENFNNQGIIDSTPTVNYKRV
TTLIKETQKKIRKILTKETINTGVEYVQGRAEVDIENKMVIVNEDDEYPFDKLLVCTGSSPYIPDIPGVHNAYTYKDTLK
INEVPEKLVIIGGGSTAAEYANIFSSLGSQVEILCRTQFLKMLNDSEAEEYIVSKLLKNTIIHENVEIQEITDTSVVTNF
GEIEGTVLLATGVVANSEIVKDIVELDSDGNILVDEFMQTSHEDIYAAGDVIGGILSTPVSRMEGICAIKNIMGKKVKPD
YSLIPLTISLSYDVSYMLGNQVSSIGKEVTLPGAAGPGSFWNTLNGKTGFTKEVVNNDGDITNILAITPSSNVALPYMIK
LIKSGSKVSDFEDFIEIHPTTDGIFKLTEYFKRYL

Sequences:

>Translated_435_residues
MTRIVIIGAGPAGRFASMAAAEVGNEVTLIENKHIGGKCLNQACMVVCALNDVSKHVLDAENFNNQGIIDSTPTVNYKRV
TTLIKETQKKIRKILTKETINTGVEYVQGRAEVDIENKMVIVNEDDEYPFDKLLVCTGSSPYIPDIPGVHNAYTYKDTLK
INEVPEKLVIIGGGSTAAEYANIFSSLGSQVEILCRTQFLKMLNDSEAEEYIVSKLLKNTIIHENVEIQEITDTSVVTNF
GEIEGTVLLATGVVANSEIVKDIVELDSDGNILVDEFMQTSHEDIYAAGDVIGGILSTPVSRMEGICAIKNIMGKKVKPD
YSLIPLTISLSYDVSYMLGNQVSSIGKEVTLPGAAGPGSFWNTLNGKTGFTKEVVNNDGDITNILAITPSSNVALPYMIK
LIKSGSKVSDFEDFIEIHPTTDGIFKLTEYFKRYL
>Mature_434_residues
TRIVIIGAGPAGRFASMAAAEVGNEVTLIENKHIGGKCLNQACMVVCALNDVSKHVLDAENFNNQGIIDSTPTVNYKRVT
TLIKETQKKIRKILTKETINTGVEYVQGRAEVDIENKMVIVNEDDEYPFDKLLVCTGSSPYIPDIPGVHNAYTYKDTLKI
NEVPEKLVIIGGGSTAAEYANIFSSLGSQVEILCRTQFLKMLNDSEAEEYIVSKLLKNTIIHENVEIQEITDTSVVTNFG
EIEGTVLLATGVVANSEIVKDIVELDSDGNILVDEFMQTSHEDIYAAGDVIGGILSTPVSRMEGICAIKNIMGKKVKPDY
SLIPLTISLSYDVSYMLGNQVSSIGKEVTLPGAAGPGSFWNTLNGKTGFTKEVVNNDGDITNILAITPSSNVALPYMIKL
IKSGSKVSDFEDFIEIHPTTDGIFKLTEYFKRYL

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI50301238, Length=338, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI91199540, Length=369, Percent_Identity=24.6612466124661, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786307, Length=365, Percent_Identity=27.1232876712329, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87082354, Length=311, Percent_Identity=24.1157556270096, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081717, Length=400, Percent_Identity=26.25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789915, Length=330, Percent_Identity=26.3636363636364, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17557007, Length=322, Percent_Identity=29.1925465838509, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983419, Length=300, Percent_Identity=27, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71983429, Length=300, Percent_Identity=27, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=23.9740820734341, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325166, Length=444, Percent_Identity=23.8738738738739, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=467, Percent_Identity=24.1970021413276, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=23.3261339092873, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=23.2217573221757, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.4

Molecular weight: Translated: 47442; Mature: 47311

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIVIIGAGPAGRFASMAAAEVGNEVTLIENKHIGGKCLNQACMVVCALNDVSKHVLDA
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ENFNNQGIIDSTPTVNYKRVTTLIKETQKKIRKILTKETINTGVEYVQGRAEVDIENKMV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEE
IVNEDDEYPFDKLLVCTGSSPYIPDIPGVHNAYTYKDTLKINEVPEKLVIIGGGSTAAEY
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCEEEECCCCCEEEEEECCCHHHHH
ANIFSSLGSQVEILCRTQFLKMLNDSEAEEYIVSKLLKNTIIHENVEIQEITDTSVVTNF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCC
GEIEGTVLLATGVVANSEIVKDIVELDSDGNILVDEFMQTSHEDIYAAGDVIGGILSTPV
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCH
SRMEGICAIKNIMGKKVKPDYSLIPLTISLSYDVSYMLGNQVSSIGKEVTLPGAAGPGSF
HHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCHH
WNTLNGKTGFTKEVVNNDGDITNILAITPSSNVALPYMIKLIKSGSKVSDFEDFIEIHPT
HHCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHEECCC
TDGIFKLTEYFKRYL
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
TRIVIIGAGPAGRFASMAAAEVGNEVTLIENKHIGGKCLNQACMVVCALNDVSKHVLDA
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ENFNNQGIIDSTPTVNYKRVTTLIKETQKKIRKILTKETINTGVEYVQGRAEVDIENKMV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEE
IVNEDDEYPFDKLLVCTGSSPYIPDIPGVHNAYTYKDTLKINEVPEKLVIIGGGSTAAEY
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCEEEECCCCCEEEEEECCCHHHHH
ANIFSSLGSQVEILCRTQFLKMLNDSEAEEYIVSKLLKNTIIHENVEIQEITDTSVVTNF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCC
GEIEGTVLLATGVVANSEIVKDIVELDSDGNILVDEFMQTSHEDIYAAGDVIGGILSTPV
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCH
SRMEGICAIKNIMGKKVKPDYSLIPLTISLSYDVSYMLGNQVSSIGKEVTLPGAAGPGSF
HHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCHH
WNTLNGKTGFTKEVVNNDGDITNILAITPSSNVALPYMIKLIKSGSKVSDFEDFIEIHPT
HHCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHEECCC
TDGIFKLTEYFKRYL
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADH [C]

Metal ions: NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {lipoamide}} 1.1 {NAD+}} [C]

Substrates: protein N6-(dihydrolipoyl)lysine; NAD+

Specific reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; p-Aminophenyl dichloroarsine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]