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The map label for this gene is trpA

Identifier: 84489869

GI number: 84489869

Start: 1215035

End: 1215844

Strand: Direct

Name: trpA

Synonym: Msp_1075

Alternate gene names: 84489869

Gene position: 1215035-1215844 (Clockwise)

Preceding gene: 84489868

Following gene: 84489870

Centisome position: 68.75

GC content: 30.0

Gene sequence:

>810_bases
ATGAGTATGAAAACATATACAGAAGTTTTTAAAAAATCTAAGGAAAATAATGAAGGAATATTCATACCATTTCTAGTGGC
AGGAGATCCTGACTATGACACATCGTTAGAAATTGCTAAAACATTGGTTGATAATGGTGCAGATGCTCTTGAAATCGGTT
TTCCATTTTCAGATCCAGTAGCTGATGGAAAAAGTGTTCAAAATGCAGACATTCGTGCATTTAACAGTGGTATGAACATT
AAAAAATGTATTAAATTCCTAAAACAATTACGTGAATATACTGATAAACCATTTGGTTTACTATTATATTATAATCTAGT
TTATAATTATGGAATTGATGCATTTTATAAGAAATTAAGTGAAATTGGAGTTAATGCCGTATTAATTGCAGATTTACCAC
CTGAAGAAGCAGATGATGTAATTGAAGCTTCTAACAAATATAATATAGAAGAAATTTTCATTGTATCACAAGCAACAAAC
AATGATAGATTAAAAATGATTACAGAAATTGTTGGTGGATTTATCTATGTTGCATCAGTTATGGGAACAACTGGTGCTAG
ATCAAATGTGGAACATAATACTACAGATTTAATAAAAAGAATTCGTAAACACACAGATATTCCATTATGTGTAGGATTTG
GTATTTCACAACCAGAACATGTAAAAGAAGTGTTAGACGCTGGTGCAGATGGTGCTATTGTTGGTAGTGCATTGATTAAT
ATTATTGAAGATAACTTAGATAATGAAGATGTTATGTTTGGATGTATAGCTGAATATATACAAAGTATGAAAGATGCTAC
AAAAAGGTAA

Upstream 100 bases:

>100_bases
CAAAACAAGAAGAAAATAAAGGAAAAACTATCATTGTAAACTTATCAGGTCGTGGAGATAAGGATATGTTTACTGTAGCA
AAAGAATTAGGGGTATCAAT

Downstream 100 bases:

>100_bases
TTAGAATGATTAGTGAAGTATTAGATGATATTATTGATAAAAATCGAAATTTAACAGTTGATGAAGCTTATGAATGTATG
GATGATTTAGTTAGTGGAAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSMKTYTEVFKKSKENNEGIFIPFLVAGDPDYDTSLEIAKTLVDNGADALEIGFPFSDPVADGKSVQNADIRAFNSGMNI
KKCIKFLKQLREYTDKPFGLLLYYNLVYNYGIDAFYKKLSEIGVNAVLIADLPPEEADDVIEASNKYNIEEIFIVSQATN
NDRLKMITEIVGGFIYVASVMGTTGARSNVEHNTTDLIKRIRKHTDIPLCVGFGISQPEHVKEVLDAGADGAIVGSALIN
IIEDNLDNEDVMFGCIAEYIQSMKDATKR

Sequences:

>Translated_269_residues
MSMKTYTEVFKKSKENNEGIFIPFLVAGDPDYDTSLEIAKTLVDNGADALEIGFPFSDPVADGKSVQNADIRAFNSGMNI
KKCIKFLKQLREYTDKPFGLLLYYNLVYNYGIDAFYKKLSEIGVNAVLIADLPPEEADDVIEASNKYNIEEIFIVSQATN
NDRLKMITEIVGGFIYVASVMGTTGARSNVEHNTTDLIKRIRKHTDIPLCVGFGISQPEHVKEVLDAGADGAIVGSALIN
IIEDNLDNEDVMFGCIAEYIQSMKDATKR
>Mature_268_residues
SMKTYTEVFKKSKENNEGIFIPFLVAGDPDYDTSLEIAKTLVDNGADALEIGFPFSDPVADGKSVQNADIRAFNSGMNIK
KCIKFLKQLREYTDKPFGLLLYYNLVYNYGIDAFYKKLSEIGVNAVLIADLPPEEADDVIEASNKYNIEEIFIVSQATNN
DRLKMITEIVGGFIYVASVMGTTGARSNVEHNTTDLIKRIRKHTDIPLCVGFGISQPEHVKEVLDAGADGAIVGSALINI
IEDNLDNEDVMFGCIAEYIQSMKDATKR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=41.1985018726592, Blast_Score=215, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321412, Length=264, Percent_Identity=35.6060606060606, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29775; Mature: 29644

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKTYTEVFKKSKENNEGIFIPFLVAGDPDYDTSLEIAKTLVDNGADALEIGFPFSDPV
CCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
ADGKSVQNADIRAFNSGMNIKKCIKFLKQLREYTDKPFGLLLYYNLVYNYGIDAFYKKLS
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHH
EIGVNAVLIADLPPEEADDVIEASNKYNIEEIFIVSQATNNDRLKMITEIVGGFIYVASV
HCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
MGTTGARSNVEHNTTDLIKRIRKHTDIPLCVGFGISQPEHVKEVLDAGADGAIVGSALIN
HHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHH
IIEDNLDNEDVMFGCIAEYIQSMKDATKR
HHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMKTYTEVFKKSKENNEGIFIPFLVAGDPDYDTSLEIAKTLVDNGADALEIGFPFSDPV
CCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
ADGKSVQNADIRAFNSGMNIKKCIKFLKQLREYTDKPFGLLLYYNLVYNYGIDAFYKKLS
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHH
EIGVNAVLIADLPPEEADDVIEASNKYNIEEIFIVSQATNNDRLKMITEIVGGFIYVASV
HCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
MGTTGARSNVEHNTTDLIKRIRKHTDIPLCVGFGISQPEHVKEVLDAGADGAIVGSALIN
HHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHH
IIEDNLDNEDVMFGCIAEYIQSMKDATKR
HHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1860817 [H]