Click here to switch to the map view.

The map label for this gene is trpE

Identifier: 84489864

GI number: 84489864

Start: 1210412

End: 1211782

Strand: Direct

Name: trpE

Synonym: Msp_1070

Alternate gene names: 84489864

Gene position: 1210412-1211782 (Clockwise)

Preceding gene: 84489862

Following gene: 84489865

Centisome position: 68.49

GC content: 29.03

Gene sequence:

>1371_bases
GTGAATGTTTTTGGCGACTTAAAAAAGCTAATTAAAAAACCAACAACTAAAAAAATTGAAATAGACAATCCAGTAAAACT
ATTTAAAAACCTATATTATAACTACAAAGACACATTTTTACTTGAATCCATGGAATCAGATAGTGGACTAGCAAGATATT
CAATAATAGGATTTAATCCAGTAGCAAAAATAAAAGCACATGATCATAATGTAACTATTACAACAGATACATATACATTT
GAATATACTTCAGACAACCCATTACTTGACATAAAAACATTGATTAAAAATGATTTTAAAGAAGAAGGATTTAGAGGAGG
ATTACTTGGATATGTTTCATATGAATCAATTAAATATTTTGAAAATGTACCAACACATAAAAGTATCTATCCTGACTTTG
AATTTGGACTATTTCTAGATTGTATTGTATATGATAATATACATAAAACATGTGAATACACAACATTAAATGAAGATAGA
AGTGATCTAATTAAAAGAATATACCAAGAAGAACATACAAATGGAATTCTTGAATATAAATTAATACAAGAAGATTTTAC
ACAAGATGAATATGAAAATGCAGTAGATGAAACTAAAAATCTAATTACTGATGGTGAAATATTCCAAGCTGTAATTTCAA
ACTCTGAAAATCTTGTTCTTAAAGGAAGTAAACTTCCCTTATATGAACACCTAAGAAAAATAAACCCATCTCATTACATG
TATCATATAAAATTAGACCAAAGAGAAATTATTGGTTCAAGTCCTGAAATGTTAATGAGACTGGAAAAAGGTACTGTTGA
ATCATATCCTATTGCAGGTACAAGGCCAAGAGGAAAAAATAAACAAGAAGACGAAGAGTTAGAAAAATCCTTACTAAATG
ATGAAAAAGAATTAGCAGAACATTTAATGCTAGTTGATTTAGCTCGTAACGATATTGGAAAAATCAGTAAAAGCAACACT
GTTAAACTTGAAGAATTCTATGGTATTAGAAAATTTTCACATGTACAACATATAGTATCACATGTAACTGGTAAATTAAA
TGACAATTTAGATGCCATTGATGCATTCATGTCATTATTCCCTGCTGGAACATTAAGTGGAGCTCCAAAAATAAGAGCAA
TGGAAATTATAAATCAAAAAGAAAAATATGCAAGAGGACCATATGGTGGTGCAGTAGGATATTTCTCATTAAATGGTAAT
GCAGACTTCGCTATTACAATAAGAACATTAACTACCAATGACAATAGGGCAGAAATACAAGCTGGTGCAGGAATTGTATA
TGATTCAATACCTCAATCAGAATATGAAGAATGTAGAAGAAAAAGACAGGCACTTTTTCAAGCTCTTAAAGAATCAGGGA
GTGAAATATGA

Upstream 100 bases:

>100_bases
TTTAATATATACGTTTAATGTATAAATTTATACACTATAACTTTATATAGTACATTGATGTAATATCTAATATAGACAAA
GGTGATATAAATGAAAAAGA

Downstream 100 bases:

>100_bases
TGATATTAATTATAGATAACTATGATTCTTTTACATACAATCTATATCAGTTAGTGGGAAAATATACTGATGATGTTGAA
GTTGTAAGAAACAATGAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNVFGDLKKLIKKPTTKKIEIDNPVKLFKNLYYNYKDTFLLESMESDSGLARYSIIGFNPVAKIKAHDHNVTITTDTYTF
EYTSDNPLLDIKTLIKNDFKEEGFRGGLLGYVSYESIKYFENVPTHKSIYPDFEFGLFLDCIVYDNIHKTCEYTTLNEDR
SDLIKRIYQEEHTNGILEYKLIQEDFTQDEYENAVDETKNLITDGEIFQAVISNSENLVLKGSKLPLYEHLRKINPSHYM
YHIKLDQREIIGSSPEMLMRLEKGTVESYPIAGTRPRGKNKQEDEELEKSLLNDEKELAEHLMLVDLARNDIGKISKSNT
VKLEEFYGIRKFSHVQHIVSHVTGKLNDNLDAIDAFMSLFPAGTLSGAPKIRAMEIINQKEKYARGPYGGAVGYFSLNGN
ADFAITIRTLTTNDNRAEIQAGAGIVYDSIPQSEYEECRRKRQALFQALKESGSEI

Sequences:

>Translated_456_residues
MNVFGDLKKLIKKPTTKKIEIDNPVKLFKNLYYNYKDTFLLESMESDSGLARYSIIGFNPVAKIKAHDHNVTITTDTYTF
EYTSDNPLLDIKTLIKNDFKEEGFRGGLLGYVSYESIKYFENVPTHKSIYPDFEFGLFLDCIVYDNIHKTCEYTTLNEDR
SDLIKRIYQEEHTNGILEYKLIQEDFTQDEYENAVDETKNLITDGEIFQAVISNSENLVLKGSKLPLYEHLRKINPSHYM
YHIKLDQREIIGSSPEMLMRLEKGTVESYPIAGTRPRGKNKQEDEELEKSLLNDEKELAEHLMLVDLARNDIGKISKSNT
VKLEEFYGIRKFSHVQHIVSHVTGKLNDNLDAIDAFMSLFPAGTLSGAPKIRAMEIINQKEKYARGPYGGAVGYFSLNGN
ADFAITIRTLTTNDNRAEIQAGAGIVYDSIPQSEYEECRRKRQALFQALKESGSEI
>Mature_456_residues
MNVFGDLKKLIKKPTTKKIEIDNPVKLFKNLYYNYKDTFLLESMESDSGLARYSIIGFNPVAKIKAHDHNVTITTDTYTF
EYTSDNPLLDIKTLIKNDFKEEGFRGGLLGYVSYESIKYFENVPTHKSIYPDFEFGLFLDCIVYDNIHKTCEYTTLNEDR
SDLIKRIYQEEHTNGILEYKLIQEDFTQDEYENAVDETKNLITDGEIFQAVISNSENLVLKGSKLPLYEHLRKINPSHYM
YHIKLDQREIIGSSPEMLMRLEKGTVESYPIAGTRPRGKNKQEDEELEKSLLNDEKELAEHLMLVDLARNDIGKISKSNT
VKLEEFYGIRKFSHVQHIVSHVTGKLNDNLDAIDAFMSLFPAGTLSGAPKIRAMEIINQKEKYARGPYGGAVGYFSLNGN
ADFAITIRTLTTNDNRAEIQAGAGIVYDSIPQSEYEECRRKRQALFQALKESGSEI

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=511, Percent_Identity=31.7025440313112, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788114, Length=413, Percent_Identity=29.5399515738499, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786809, Length=288, Percent_Identity=26.7361111111111, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082077, Length=251, Percent_Identity=25.0996015936255, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320935, Length=444, Percent_Identity=35.5855855855856, Blast_Score=236, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6324361, Length=401, Percent_Identity=23.1920199501247, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR010116
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 52199; Mature: 52199

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFGDLKKLIKKPTTKKIEIDNPVKLFKNLYYNYKDTFLLESMESDSGLARYSIIGFNP
CCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCHHEEEECCCCCCCCEEEEEEECCC
VAKIKAHDHNVTITTDTYTFEYTSDNPLLDIKTLIKNDFKEEGFRGGLLGYVSYESIKYF
EEEEEECCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHH
ENVPTHKSIYPDFEFGLFLDCIVYDNIHKTCEYTTLNEDRSDLIKRIYQEEHTNGILEYK
HCCCCCCCCCCCCCHHHHHHHHHHHCHHHCEEEEECCCCHHHHHHHHHHHHCCCCEEEEE
LIQEDFTQDEYENAVDETKNLITDGEIFQAVISNSENLVLKGSKLPLYEHLRKINPSHYM
EHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCEE
YHIKLDQREIIGSSPEMLMRLEKGTVESYPIAGTRPRGKNKQEDEELEKSLLNDEKELAE
EEEEECHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHH
HLMLVDLARNDIGKISKSNTVKLEEFYGIRKFSHVQHIVSHVTGKLNDNLDAIDAFMSLF
HHHHHHHHHHHHHCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PAGTLSGAPKIRAMEIINQKEKYARGPYGGAVGYFSLNGNADFAITIRTLTTNDNRAEIQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCCEEEE
AGAGIVYDSIPQSEYEECRRKRQALFQALKESGSEI
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVFGDLKKLIKKPTTKKIEIDNPVKLFKNLYYNYKDTFLLESMESDSGLARYSIIGFNP
CCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCHHEEEECCCCCCCCEEEEEEECCC
VAKIKAHDHNVTITTDTYTFEYTSDNPLLDIKTLIKNDFKEEGFRGGLLGYVSYESIKYF
EEEEEECCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHH
ENVPTHKSIYPDFEFGLFLDCIVYDNIHKTCEYTTLNEDRSDLIKRIYQEEHTNGILEYK
HCCCCCCCCCCCCCHHHHHHHHHHHCHHHCEEEEECCCCHHHHHHHHHHHHCCCCEEEEE
LIQEDFTQDEYENAVDETKNLITDGEIFQAVISNSENLVLKGSKLPLYEHLRKINPSHYM
EHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCEE
YHIKLDQREIIGSSPEMLMRLEKGTVESYPIAGTRPRGKNKQEDEELEKSLLNDEKELAE
EEEEECHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHH
HLMLVDLARNDIGKISKSNTVKLEEFYGIRKFSHVQHIVSHVTGKLNDNLDAIDAFMSLF
HHHHHHHHHHHHHCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PAGTLSGAPKIRAMEIINQKEKYARGPYGGAVGYFSLNGNADFAITIRTLTTNDNRAEIQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCCEEEE
AGAGIVYDSIPQSEYEECRRKRQALFQALKESGSEI
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]