Click here to switch to the map view.

The map label for this gene is purA

Identifier: 84489785

GI number: 84489785

Start: 1134986

End: 1136011

Strand: Direct

Name: purA

Synonym: Msp_0986

Alternate gene names: 84489785

Gene position: 1134986-1136011 (Clockwise)

Preceding gene: 84489783

Following gene: 84489786

Centisome position: 64.22

GC content: 33.24

Gene sequence:

>1026_bases
ATGACATGTACCATATTAGTTGGTGGACAATGGGGTGACGAAGGTAAAGGAAAATGTATAACATACTTTTGTACCCAAGA
TAAACCTGAAATTATTGCTAGAGCAGGAGTAGGTCCAAATGCTGGACACTCAGTAGAATTTAATGGTGAAAAATACGGTT
TAAGATTAACACCATCAGGTTTTTTCAATAAAGAAGCAAGATTATTAATTGGTGCTGGAGTTTTAGTAAATCCAGAAGTA
TTTGAACACGAATTAGAATACCTTAGCAAATATGCAGTTGAAGGTAGAACATTCATGGACGCAAACTGTGCAATAATCAC
AGACAAACACACAAAACAGGATAAAGATTCAGCATATTTATCTAAAAAAATAGGAACAACTGGAAGTGGATGTGGACCTG
CAAATGCAGATAGAATAAACAGGACAATAGACTATGCAAAAGATGTACCAGAACTAGAAAAATACATTACAGATGTACCT
TCAGAAATAAACAAGGCAATTGATGAAGGTAAAGATGTATTTATTGAAGGATCACAAGGATTTGGATTATCATTATACTA
TGGAACATACCCCTATGTAACAAGTAAAGATACATGTGCAAGTACTGCGGCAGCAGATGTAGGTGTAGGACCAACAAAAG
TAGATGAAGTAATAGTAATATTTAAGTCATACATTACAAGAGTAGGTGAAGGACCATTCCCAACAGAAATTTCACCAGAA
GAAGCTGAAAAAATGGGAATAGAAGAATATGGTGTAGTAACAGGTCGTAAAAGAAGAGTAGGATTATTTGATAAAGACTT
TGCAAAAAGATCATGTATGATAAATGGTGCTACACAAATAGCTCTAACATGTATTGATAGATTATATCCAGAATGTGCTA
AAGTAAATAAATATGAAGATTTATCACAGGAAGCTAGAGATTATATTGAAGATATTGAAGAAAATGTTGGTGTTCCAATT
ACTATCATATCCACAGGTCCTGATCTTGCAGATACAATTGATTTAAGAAATGAAAAATTAAATTAA

Upstream 100 bases:

>100_bases
GTAAAAGACTTTATAAAAAAAATTTTATAAAATCAAAGTAATACTTCCACTATAAAAACAGGAAGTATTGGAAATTATTA
TTTAAAAGGAGATACAATTC

Downstream 100 bases:

>100_bases
TCTCCTTTACTTTTTTTTAAAAAAAATATTTTATTAATATTTAATTATTTAGATGAATATTTCTTAATTAACTAATTCTT
TTGATTTATTTTTATTATAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTCTILVGGQWGDEGKGKCITYFCTQDKPEIIARAGVGPNAGHSVEFNGEKYGLRLTPSGFFNKEARLLIGAGVLVNPEV
FEHELEYLSKYAVEGRTFMDANCAIITDKHTKQDKDSAYLSKKIGTTGSGCGPANADRINRTIDYAKDVPELEKYITDVP
SEINKAIDEGKDVFIEGSQGFGLSLYYGTYPYVTSKDTCASTAAADVGVGPTKVDEVIVIFKSYITRVGEGPFPTEISPE
EAEKMGIEEYGVVTGRKRRVGLFDKDFAKRSCMINGATQIALTCIDRLYPECAKVNKYEDLSQEARDYIEDIEENVGVPI
TIISTGPDLADTIDLRNEKLN

Sequences:

>Translated_341_residues
MTCTILVGGQWGDEGKGKCITYFCTQDKPEIIARAGVGPNAGHSVEFNGEKYGLRLTPSGFFNKEARLLIGAGVLVNPEV
FEHELEYLSKYAVEGRTFMDANCAIITDKHTKQDKDSAYLSKKIGTTGSGCGPANADRINRTIDYAKDVPELEKYITDVP
SEINKAIDEGKDVFIEGSQGFGLSLYYGTYPYVTSKDTCASTAAADVGVGPTKVDEVIVIFKSYITRVGEGPFPTEISPE
EAEKMGIEEYGVVTGRKRRVGLFDKDFAKRSCMINGATQIALTCIDRLYPECAKVNKYEDLSQEARDYIEDIEENVGVPI
TIISTGPDLADTIDLRNEKLN
>Mature_340_residues
TCTILVGGQWGDEGKGKCITYFCTQDKPEIIARAGVGPNAGHSVEFNGEKYGLRLTPSGFFNKEARLLIGAGVLVNPEVF
EHELEYLSKYAVEGRTFMDANCAIITDKHTKQDKDSAYLSKKIGTTGSGCGPANADRINRTIDYAKDVPELEKYITDVPS
EINKAIDEGKDVFIEGSQGFGLSLYYGTYPYVTSKDTCASTAAADVGVGPTKVDEVIVIFKSYITRVGEGPFPTEISPEE
AEKMGIEEYGVVTGRKRRVGLFDKDFAKRSCMINGATQIALTCIDRLYPECAKVNKYEDLSQEARDYIEDIEENVGVPIT
IISTGPDLADTIDLRNEKLN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=28.8056206088993, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI34577063, Length=339, Percent_Identity=30.6784660766962, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=27.5510204081633, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=33.0985915492958, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=33.4905660377358, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=33.4905660377358, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=30.9692671394799, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21356033, Length=357, Percent_Identity=30.2521008403361, Blast_Score=150, Evalue=9e-37,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_METST (Q2NFM9)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448017.1
- ProteinModelPortal:   Q2NFM9
- SMR:   Q2NFM9
- STRING:   Q2NFM9
- GeneID:   3855974
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0986
- NMPDR:   fig|339860.6.peg.945
- eggNOG:   arNOG04146
- HOGENOM:   HBG658237
- OMA:   IDPHVGI
- ProtClustDB:   PRK04293
- BioCyc:   MSTA339860:MSP_0986-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 37346; Mature: 37215

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 43-43 BINDING 127-127 BINDING 141-141 BINDING 179-179 BINDING 194-194 BINDING 256-256 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCTILVGGQWGDEGKGKCITYFCTQDKPEIIARAGVGPNAGHSVEFNGEKYGLRLTPSG
CEEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEECCCEEEEEECCCC
FFNKEARLLIGAGVLVNPEVFEHELEYLSKYAVEGRTFMDANCAIITDKHTKQDKDSAYL
CCCCCCEEEEECCEEECHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHHHH
SKKIGTTGSGCGPANADRINRTIDYAKDVPELEKYITDVPSEINKAIDEGKDVFIEGSQG
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCC
FGLSLYYGTYPYVTSKDTCASTAAADVGVGPTKVDEVIVIFKSYITRVGEGPFPTEISPE
CEEEEEECCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EAEKMGIEEYGVVTGRKRRVGLFDKDFAKRSCMINGATQIALTCIDRLYPECAKVNKYED
HHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHEEECCHHHHHHHHHHHHCHHHHHCCCHHH
LSQEARDYIEDIEENVGVPITIISTGPDLADTIDLRNEKLN
HHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TCTILVGGQWGDEGKGKCITYFCTQDKPEIIARAGVGPNAGHSVEFNGEKYGLRLTPSG
EEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEECCCEEEEEECCCC
FFNKEARLLIGAGVLVNPEVFEHELEYLSKYAVEGRTFMDANCAIITDKHTKQDKDSAYL
CCCCCCEEEEECCEEECHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHHHH
SKKIGTTGSGCGPANADRINRTIDYAKDVPELEKYITDVPSEINKAIDEGKDVFIEGSQG
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCC
FGLSLYYGTYPYVTSKDTCASTAAADVGVGPTKVDEVIVIFKSYITRVGEGPFPTEISPE
CEEEEEECCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EAEKMGIEEYGVVTGRKRRVGLFDKDFAKRSCMINGATQIALTCIDRLYPECAKVNKYED
HHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHEEECCHHHHHHHHHHHHCHHHHHCCCHHH
LSQEARDYIEDIEENVGVPITIISTGPDLADTIDLRNEKLN
HHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA