Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 84489722

GI number: 84489722

Start: 1061903

End: 1063594

Strand: Direct

Name: gltX

Synonym: Msp_0923

Alternate gene names: 84489722

Gene position: 1061903-1063594 (Clockwise)

Preceding gene: 84489717

Following gene: 84489723

Centisome position: 60.08

GC content: 31.5

Gene sequence:

>1692_bases
ATGGATATAGAAGAAACTATTTATAAATATGCTTTAACTAATGCTATAGAACATGGAAATAAATGTCAAGTAGGATCAGT
AATAGGAATGGTAATGTCTAAAAACCCAGAAATGAGAAAAGACCCCAAAACTGTATCACAACTTGCAGGAAAATTAGTTG
CAAAAGTAAATAATTTAACACCAGAAGAACAACAAAAAGAAGTGGAATCACTTGGTGGACTAGAACAACATACAAAAAAA
GAAGAAAAACCGAAAGGATTACCTGAATTAAAAAATACTGAAGATAAAAAAGTAACACTAAGATTTGCACCAAATCCATC
AGGACCACTTCATATTGGACATGCAAGAGCAGCAATACTAAACAAACTATACCAGGAAAAATACAATGGAAAACTAATTC
TTAGAATTGAAGATACAGATCCAAAAAGAGTAGAACCAGATGCATATGATGCAATACCACAAGATATTAAATGGTTAGGT
ATAGAACCAGATGAAATATACACCCAAAGTGACAGACTAGAAATATACTATGAATATGCACAAAAAGCAATAGAAATAGG
TGCTGCATATATGTGTAAATGTGATGGTGGAGAATTTAAAAAACTTAAAGATAACTGTCAACCATGTCCATGTAGAAGTC
ATACCATAGAAGAAAACATGGAATTATGGAATAACTTTGAAAATATGGAAGAAGGAGAAGCAGTACTTCGTATAAAAACA
GATATAAATCATAAAAATCCTGCAATACGTGACTGGGTTGCAATGAGAATAGTAAACCAAGAACATCCAAGATTAGGAAA
TAAATATAAAATCTATCCAATGATGAACTTCTCAGTAACAGTAGATGATCATCTTATGGGAATGACACATGTATTACGTG
GAAAAGACCATCTTGCAAACAGTGAAAAACAAACATACCTATACAAACACTTTGGATGGGATGTACCAGAGTTTATTCAC
TATGGTAGATTAAAAATGGATGATGTAGAACTTAGTACATCTAAAGCAAGAGAAGGTATTGAAAATAAAACATACACTGG
ATGGGATGATCCAAGACTTGGAACTATACGTGCAATTGCAAGAAGAGGAATTAAAAAAGAAGTACTTTATGATTTAATAG
AGGAGATTGGAACAAAACAAGCCGATGCAACAATAAGTTGGAAGAAAATCTATGGATTAAATCGTAACATCATAGAGGAA
AACACAAACAGATACTTCTTCATACCAAATGCTGTTAAAGTAGATGTTGAAAATCTACCAAGTTCTAAAACAAACATGAC
AGTAGAACGTGAACTTCACTATAACAAACCAGAAAAAGGATTTAGAAACTTAACATTTAATGGTAGTGTATACATACCAG
AAGATGACTATAAACATGCACAAGATAAAAACATACCATTAAGACTAATGGATTTAATAAATATTAAAATAGAGGAAGAT
AAAGTAATATATGATAGTGAAACTCTTGAAGATGCACAAGCACAACATGCACGTATTATACAATGGGCACCAGTTGAAAC
ATCCATAAGTGCTACAGTTGTAATGCCAGATAATACTCATGTTACAGGATATATTGAATGTGATGCATCAAATCTAGAAG
TTGATGATATGGTACAACTTGAAAGATTTGGATTTGCAAGAGTAGATAAAATAAATGATAATGAAATAACATTCTATTAT
ACACATAACTAA

Upstream 100 bases:

>100_bases
TTTAGACATATATAATATACAATAAAAAGAAAAATTTTTCAAAAAAAATAACCACCCTCTTTTTTATAATAATTAAATTA
CACAAAAACGGAGAATATCA

Downstream 100 bases:

>100_bases
AAAGGTGAAATATATGGCAGTGAAAGTAAATGAATACTATAGTTTAATACAAAACAACTACTTATTTGTAGAAATTGCAA
GAAGAGTAGATGAATATCAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MDIEETIYKYALTNAIEHGNKCQVGSVIGMVMSKNPEMRKDPKTVSQLAGKLVAKVNNLTPEEQQKEVESLGGLEQHTKK
EEKPKGLPELKNTEDKKVTLRFAPNPSGPLHIGHARAAILNKLYQEKYNGKLILRIEDTDPKRVEPDAYDAIPQDIKWLG
IEPDEIYTQSDRLEIYYEYAQKAIEIGAAYMCKCDGGEFKKLKDNCQPCPCRSHTIEENMELWNNFENMEEGEAVLRIKT
DINHKNPAIRDWVAMRIVNQEHPRLGNKYKIYPMMNFSVTVDDHLMGMTHVLRGKDHLANSEKQTYLYKHFGWDVPEFIH
YGRLKMDDVELSTSKAREGIENKTYTGWDDPRLGTIRAIARRGIKKEVLYDLIEEIGTKQADATISWKKIYGLNRNIIEE
NTNRYFFIPNAVKVDVENLPSSKTNMTVERELHYNKPEKGFRNLTFNGSVYIPEDDYKHAQDKNIPLRLMDLINIKIEED
KVIYDSETLEDAQAQHARIIQWAPVETSISATVVMPDNTHVTGYIECDASNLEVDDMVQLERFGFARVDKINDNEITFYY
THN

Sequences:

>Translated_563_residues
MDIEETIYKYALTNAIEHGNKCQVGSVIGMVMSKNPEMRKDPKTVSQLAGKLVAKVNNLTPEEQQKEVESLGGLEQHTKK
EEKPKGLPELKNTEDKKVTLRFAPNPSGPLHIGHARAAILNKLYQEKYNGKLILRIEDTDPKRVEPDAYDAIPQDIKWLG
IEPDEIYTQSDRLEIYYEYAQKAIEIGAAYMCKCDGGEFKKLKDNCQPCPCRSHTIEENMELWNNFENMEEGEAVLRIKT
DINHKNPAIRDWVAMRIVNQEHPRLGNKYKIYPMMNFSVTVDDHLMGMTHVLRGKDHLANSEKQTYLYKHFGWDVPEFIH
YGRLKMDDVELSTSKAREGIENKTYTGWDDPRLGTIRAIARRGIKKEVLYDLIEEIGTKQADATISWKKIYGLNRNIIEE
NTNRYFFIPNAVKVDVENLPSSKTNMTVERELHYNKPEKGFRNLTFNGSVYIPEDDYKHAQDKNIPLRLMDLINIKIEED
KVIYDSETLEDAQAQHARIIQWAPVETSISATVVMPDNTHVTGYIECDASNLEVDDMVQLERFGFARVDKINDNEITFYY
THN
>Mature_563_residues
MDIEETIYKYALTNAIEHGNKCQVGSVIGMVMSKNPEMRKDPKTVSQLAGKLVAKVNNLTPEEQQKEVESLGGLEQHTKK
EEKPKGLPELKNTEDKKVTLRFAPNPSGPLHIGHARAAILNKLYQEKYNGKLILRIEDTDPKRVEPDAYDAIPQDIKWLG
IEPDEIYTQSDRLEIYYEYAQKAIEIGAAYMCKCDGGEFKKLKDNCQPCPCRSHTIEENMELWNNFENMEEGEAVLRIKT
DINHKNPAIRDWVAMRIVNQEHPRLGNKYKIYPMMNFSVTVDDHLMGMTHVLRGKDHLANSEKQTYLYKHFGWDVPEFIH
YGRLKMDDVELSTSKAREGIENKTYTGWDDPRLGTIRAIARRGIKKEVLYDLIEEIGTKQADATISWKKIYGLNRNIIEE
NTNRYFFIPNAVKVDVENLPSSKTNMTVERELHYNKPEKGFRNLTFNGSVYIPEDDYKHAQDKNIPLRLMDLINIKIEED
KVIYDSETLEDAQAQHARIIQWAPVETSISATVVMPDNTHVTGYIECDASNLEVDDMVQLERFGFARVDKINDNEITFYY
THN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=369, Percent_Identity=33.6043360433604, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4826960, Length=364, Percent_Identity=29.9450549450549, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI134288884, Length=244, Percent_Identity=33.6065573770492, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1786895, Length=458, Percent_Identity=28.6026200873362, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1788743, Length=246, Percent_Identity=32.520325203252, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI226510925, Length=239, Percent_Identity=29.2887029288703, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17511007, Length=504, Percent_Identity=28.7698412698413, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17543332, Length=510, Percent_Identity=28.4313725490196, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=34.2629482071713, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324742, Length=474, Percent_Identity=30.379746835443, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321192, Length=526, Percent_Identity=26.2357414448669, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=345, Percent_Identity=28.4057971014493, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649466, Length=418, Percent_Identity=29.6650717703349, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI17864488, Length=513, Percent_Identity=27.8752436647173, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24665321, Length=274, Percent_Identity=34.3065693430657, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_METST (Q2NFU2)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447954.1
- ProteinModelPortal:   Q2NFU2
- SMR:   Q2NFU2
- STRING:   Q2NFU2
- GeneID:   3856230
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0923
- NMPDR:   fig|339860.6.peg.887
- eggNOG:   arNOG04636
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- PhylomeDB:   Q2NFU2
- ProtClustDB:   PRK04156
- BioCyc:   MSTA339860:MSP_0923-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR001412
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64969; Mature: 64969

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEETIYKYALTNAIEHGNKCQVGSVIGMVMSKNPEMRKDPKTVSQLAGKLVAKVNNLT
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PEEQQKEVESLGGLEQHTKKEEKPKGLPELKNTEDKKVTLRFAPNPSGPLHIGHARAAIL
CHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECHHHHHHH
NKLYQEKYNGKLILRIEDTDPKRVEPDAYDAIPQDIKWLGIEPDEIYTQSDRLEIYYEYA
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEECCCHHHHCCCCCCEEEEHHHH
QKAIEIGAAYMCKCDGGEFKKLKDNCQPCPCRSHTIEENMELWNNFENMEEGEAVLRIKT
HHHHHHCCEEEEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEE
DINHKNPAIRDWVAMRIVNQEHPRLGNKYKIYPMMNFSVTVDDHLMGMTHVLRGKDHLAN
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHCCCHHHCC
SEKQTYLYKHFGWDVPEFIHYGRLKMDDVELSTSKAREGIENKTYTGWDDPRLGTIRAIA
CCHHEEEEHHCCCCHHHHHHCCCCEECCEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHH
RRGIKKEVLYDLIEEIGTKQADATISWKKIYGLNRNIIEENTNRYFFIPNAVKVDVENLP
HCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCHHHCCCCEEEECCCCEEEEHHHCC
SSKTNMTVERELHYNKPEKGFRNLTFNGSVYIPEDDYKHAQDKNIPLRLMDLINIKIEED
CCCCCEEEEEEECCCCCCCHHCEEEECCEEEECCCCCCCCCCCCCCEEEEEEEEEEECCC
KVIYDSETLEDAQAQHARIIQWAPVETSISATVVMPDNTHVTGYIECDASNLEVDDMVQL
EEEECCCHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHH
ERFGFARVDKINDNEITFYYTHN
HHCCCEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MDIEETIYKYALTNAIEHGNKCQVGSVIGMVMSKNPEMRKDPKTVSQLAGKLVAKVNNLT
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PEEQQKEVESLGGLEQHTKKEEKPKGLPELKNTEDKKVTLRFAPNPSGPLHIGHARAAIL
CHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECHHHHHHH
NKLYQEKYNGKLILRIEDTDPKRVEPDAYDAIPQDIKWLGIEPDEIYTQSDRLEIYYEYA
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEECCCHHHHCCCCCCEEEEHHHH
QKAIEIGAAYMCKCDGGEFKKLKDNCQPCPCRSHTIEENMELWNNFENMEEGEAVLRIKT
HHHHHHCCEEEEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEE
DINHKNPAIRDWVAMRIVNQEHPRLGNKYKIYPMMNFSVTVDDHLMGMTHVLRGKDHLAN
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHCCCHHHCC
SEKQTYLYKHFGWDVPEFIHYGRLKMDDVELSTSKAREGIENKTYTGWDDPRLGTIRAIA
CCHHEEEEHHCCCCHHHHHHCCCCEECCEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHH
RRGIKKEVLYDLIEEIGTKQADATISWKKIYGLNRNIIEENTNRYFFIPNAVKVDVENLP
HCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCHHHCCCCEEEECCCCEEEEHHHCC
SSKTNMTVERELHYNKPEKGFRNLTFNGSVYIPEDDYKHAQDKNIPLRLMDLINIKIEED
CCCCCEEEEEEECCCCCCCHHCEEEECCEEEECCCCCCCCCCCCCCEEEEEEEEEEECCC
KVIYDSETLEDAQAQHARIIQWAPVETSISATVVMPDNTHVTGYIECDASNLEVDDMVQL
EEEECCCHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHH
ERFGFARVDKINDNEITFYYTHN
HHCCCEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA