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The map label for this gene is phoA [H]

Identifier: 83721540

GI number: 83721540

Start: 370094

End: 371494

Strand: Direct

Name: phoA [H]

Synonym: BTH_I0333

Alternate gene names: 83721540

Gene position: 370094-371494 (Clockwise)

Preceding gene: 83720832

Following gene: 83719237

Centisome position: 9.72

GC content: 68.81

Gene sequence:

>1401_bases
ATGTCGACTATCAAGCGCATCGCGGCGGCGTTCGCCGCGGCGGCCCTTTCGGGCGGTGTCGGCCAGGCGGCGCACGCCGC
GGGCCAGGCGAAGAACGTGATCTTCTTCCTCGGCGACGGGATGGGGCCGGCCACCGTGACGGCGAGCCGCCTCTACAAGG
TCGGCGAGGCCGGCCAACTGACGATGGAGACGCTGCCGCGCACCGCGCGCATCAAGACTTTCTCGAACGACGCGCAGACC
ACCGACAGCGCGCCGTCGATGGCCGCGTACATGACCGGCGTGAAGATGAACAACGAGGTGCTGTCGATGTCGCCGGACAC
GCGCGCGGTCGCGCCCGGCAGCGACGCGAACGGCAACAAGACGGTGAACAACTGCGGCGCGAACAACGGCAAGCCGGTGC
CGACGCTGCTCGAGCTCGCGAAGGCGCGGGGCAAGGCGGTCGGCGCGATCACGACGACCGAGCTCACGCATGCGACGCCC
GCCGCGACGTACTCGCACATCTGCCATCGCGATGCGCAGTACGACATCGCCGCGCAGGCGGTGCCGGGCGGCGCGGGCTA
CAACGCGGCGCTCGGCGACGGCGTCGACGTGCTGATGGGCGGCGGCCGCAATCACTGGACGCCGTACGATCCGGTCGCGA
ACAAGCGCGGCCGCGCGGACGGGCGCAATCTGCTCGCCGAGCTGCAGGCGAAGGGCTACGCGGTCGCGGCGACGAAGGAC
CAGCTCGCGCAGGCGGGCGGCGGCAAGCTGATCGGGCTCTTCAGCGCGACGAGCCATCTCGAGTACGAGCTCGATCGCGT
CGCGGGCAAGGGCGAGGGCGCGACGCAGCCGAGCCTTGCGGAGATGACCGCGAAGGCGATCGACGTGTTGCGGAAGAATC
CGAACGGCTATTTCCTGATGGTCGAAGGCGGGCGCATCGATCACGCGCTGCACGGCACGAACGCAAAGCGCGCGCTCGAA
GACACGGTGGCGTTCGACGAGGCGATTCGCACCGCGCTCGCGAAGGTCGATCTGTCGAACACGCTGATCGTCGTGACGGC
CGACCACGACCACACGTTGACGATCAACGGCTATGCGAAGCGCGGCAATCCGGTGCTCGACATCAGCCGCAGCTATCGCG
ACGGCCAGCCGAACCGGGACGCGGACGGCAACACGTATACGACGCTCGTGTTCGGCAACGGCGCGAATCGCCCGAACGTG
CGCACGCCGGTGGATTCGGCGAGCGCGACGAACGAAGCGTACCTGCAGGAAGTCGGCGTGCGGATGGGCGGCGCGGGCTC
CGAGACGCACGGCGGCGGGGACGTGATGCTGTTCGCCGACGGCGCCGACGCGAAGGCGTTCAAGGGCACGCTCGACAACA
CGAAGGTGTTCGGCCTCGTGAAATCGGTGTTCGGGTTTTGA

Upstream 100 bases:

>100_bases
GAGGGCTTTCACGGCGTGATCGAGAACAACAAGGTGTTCGAGCTCGTGCGCGGCGCGGCGCAGCTCTGAGCGCGGCCTCG
ACGGAACAGGGGAATACAGG

Downstream 100 bases:

>100_bases
GCGCAACGTTTGCGATGCGGACGTTCGCGATGAAACAGATCGTCGCGCGGCGCGTGGGCGCGGCGTTCGGCGCGATCGCG
ATGCTCGCCGCCGCGCCGGC

Product: alkaline phosphatase family protein

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSTIKRIAAAFAAAALSGGVGQAAHAAGQAKNVIFFLGDGMGPATVTASRLYKVGEAGQLTMETLPRTARIKTFSNDAQT
TDSAPSMAAYMTGVKMNNEVLSMSPDTRAVAPGSDANGNKTVNNCGANNGKPVPTLLELAKARGKAVGAITTTELTHATP
AATYSHICHRDAQYDIAAQAVPGGAGYNAALGDGVDVLMGGGRNHWTPYDPVANKRGRADGRNLLAELQAKGYAVAATKD
QLAQAGGGKLIGLFSATSHLEYELDRVAGKGEGATQPSLAEMTAKAIDVLRKNPNGYFLMVEGGRIDHALHGTNAKRALE
DTVAFDEAIRTALAKVDLSNTLIVVTADHDHTLTINGYAKRGNPVLDISRSYRDGQPNRDADGNTYTTLVFGNGANRPNV
RTPVDSASATNEAYLQEVGVRMGGAGSETHGGGDVMLFADGADAKAFKGTLDNTKVFGLVKSVFGF

Sequences:

>Translated_466_residues
MSTIKRIAAAFAAAALSGGVGQAAHAAGQAKNVIFFLGDGMGPATVTASRLYKVGEAGQLTMETLPRTARIKTFSNDAQT
TDSAPSMAAYMTGVKMNNEVLSMSPDTRAVAPGSDANGNKTVNNCGANNGKPVPTLLELAKARGKAVGAITTTELTHATP
AATYSHICHRDAQYDIAAQAVPGGAGYNAALGDGVDVLMGGGRNHWTPYDPVANKRGRADGRNLLAELQAKGYAVAATKD
QLAQAGGGKLIGLFSATSHLEYELDRVAGKGEGATQPSLAEMTAKAIDVLRKNPNGYFLMVEGGRIDHALHGTNAKRALE
DTVAFDEAIRTALAKVDLSNTLIVVTADHDHTLTINGYAKRGNPVLDISRSYRDGQPNRDADGNTYTTLVFGNGANRPNV
RTPVDSASATNEAYLQEVGVRMGGAGSETHGGGDVMLFADGADAKAFKGTLDNTKVFGLVKSVFGF
>Mature_465_residues
STIKRIAAAFAAAALSGGVGQAAHAAGQAKNVIFFLGDGMGPATVTASRLYKVGEAGQLTMETLPRTARIKTFSNDAQTT
DSAPSMAAYMTGVKMNNEVLSMSPDTRAVAPGSDANGNKTVNNCGANNGKPVPTLLELAKARGKAVGAITTTELTHATPA
ATYSHICHRDAQYDIAAQAVPGGAGYNAALGDGVDVLMGGGRNHWTPYDPVANKRGRADGRNLLAELQAKGYAVAATKDQ
LAQAGGGKLIGLFSATSHLEYELDRVAGKGEGATQPSLAEMTAKAIDVLRKNPNGYFLMVEGGRIDHALHGTNAKRALED
TVAFDEAIRTALAKVDLSNTLIVVTADHDHTLTINGYAKRGNPVLDISRSYRDGQPNRDADGNTYTTLVFGNGANRPNVR
TPVDSASATNEAYLQEVGVRMGGAGSETHGGGDVMLFADGADAKAFKGTLDNTKVFGLVKSVFGF

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=455, Percent_Identity=35.1648351648352, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI157266296, Length=477, Percent_Identity=35.2201257861635, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI94721246, Length=477, Percent_Identity=35.2201257861635, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI116734717, Length=458, Percent_Identity=35.3711790393013, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI294660770, Length=453, Percent_Identity=34.6578366445916, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI294660772, Length=409, Percent_Identity=33.4963325183374, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI48994877, Length=365, Percent_Identity=34.2465753424658, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=348, Percent_Identity=33.3333333333333, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21355151, Length=458, Percent_Identity=34.2794759825328, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21358067, Length=464, Percent_Identity=34.2672413793103, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI21355149, Length=456, Percent_Identity=33.9912280701754, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI85725200, Length=457, Percent_Identity=33.9168490153173, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI85815833, Length=457, Percent_Identity=33.9168490153173, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI24585213, Length=464, Percent_Identity=33.6206896551724, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI19921912, Length=481, Percent_Identity=34.0956340956341, Blast_Score=208, Evalue=6e-54,
Organism=Drosophila melanogaster, GI18859923, Length=459, Percent_Identity=33.7690631808279, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI21355981, Length=455, Percent_Identity=33.8461538461538, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24651554, Length=474, Percent_Identity=32.9113924050633, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24657827, Length=464, Percent_Identity=33.6206896551724, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24657835, Length=462, Percent_Identity=32.6839826839827, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24657842, Length=465, Percent_Identity=31.6129032258064, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24651556, Length=461, Percent_Identity=32.3210412147505, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 48399; Mature: 48268

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIKRIAAAFAAAALSGGVGQAAHAAGQAKNVIFFLGDGMGPATVTASRLYKVGEAGQL
CCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCE
TMETLPRTARIKTFSNDAQTTDSAPSMAAYMTGVKMNNEVLSMSPDTRAVAPGSDANGNK
EHHHCCCCEEEEEECCCCCCCCCCCCHHHHHCCCEECCCEEEECCCCCEECCCCCCCCCC
TVNNCGANNGKPVPTLLELAKARGKAVGAITTTELTHATPAATYSHICHRDAQYDIAAQA
CHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEE
VPGGAGYNAALGDGVDVLMGGGRNHWTPYDPVANKRGRADGRNLLAELQAKGYAVAATKD
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECHH
QLAQAGGGKLIGLFSATSHLEYELDRVAGKGEGATQPSLAEMTAKAIDVLRKNPNGYFLM
HHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
VEGGRIDHALHGTNAKRALEDTVAFDEAIRTALAKVDLSNTLIVVTADHDHTLTINGYAK
EECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCEEEEECCCC
RGNPVLDISRSYRDGQPNRDADGNTYTTLVFGNGANRPNVRTPVDSASATNEAYLQEVGV
CCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RMGGAGSETHGGGDVMLFADGADAKAFKGTLDNTKVFGLVKSVFGF
HCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
STIKRIAAAFAAAALSGGVGQAAHAAGQAKNVIFFLGDGMGPATVTASRLYKVGEAGQL
CHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCE
TMETLPRTARIKTFSNDAQTTDSAPSMAAYMTGVKMNNEVLSMSPDTRAVAPGSDANGNK
EHHHCCCCEEEEEECCCCCCCCCCCCHHHHHCCCEECCCEEEECCCCCEECCCCCCCCCC
TVNNCGANNGKPVPTLLELAKARGKAVGAITTTELTHATPAATYSHICHRDAQYDIAAQA
CHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEE
VPGGAGYNAALGDGVDVLMGGGRNHWTPYDPVANKRGRADGRNLLAELQAKGYAVAATKD
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECHH
QLAQAGGGKLIGLFSATSHLEYELDRVAGKGEGATQPSLAEMTAKAIDVLRKNPNGYFLM
HHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
VEGGRIDHALHGTNAKRALEDTVAFDEAIRTALAKVDLSNTLIVVTADHDHTLTINGYAK
EECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCEEEEECCCC
RGNPVLDISRSYRDGQPNRDADGNTYTTLVFGNGANRPNVRTPVDSASATNEAYLQEVGV
CCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RMGGAGSETHGGGDVMLFADGADAKAFKGTLDNTKVFGLVKSVFGF
HCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]