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The map label for this gene is dusA [H]

Identifier: 83721523

GI number: 83721523

Start: 2852135

End: 2853154

Strand: Direct

Name: dusA [H]

Synonym: BTH_I2494

Alternate gene names: 83721523

Gene position: 2852135-2853154 (Clockwise)

Preceding gene: 83719924

Following gene: 83719520

Centisome position: 74.87

GC content: 65.2

Gene sequence:

>1020_bases
ATGTCCGCTTCGCCTGCCCTCAGTCCGCGCCGGGTGTCCGTCGCCCCGATGATGGACTGGACCGATCGCCACTGCCGTTC
GTTTCATCGGACGATCTCGCGCCATGCATGGCTCTACACCGAGATGGTGACGACGGGCGCGTTGATCCACGGCGACGTCG
CGCGCCATCTGGCATTCACCCCCGACGAAGCGCCCGTCGCATTGCAACTGGGCGGCAGCGAGCCCGCCGAGCTCGCGCAT
TCCGCGAGACTCGGCGAACGCTGGGGCTACGACGAAATCAACCTGAATTGCGGTTGCCCGTCCGAGCGCGTGCAGCGCGG
CGCATTCGGCGCATGCCTGATGAACGAGCCGCAGCTCGTCGCGGATTGCGTGAAGGCGATGCGCGACGCGGTGTCGATTC
CGGTGACGGTCAAGCACCGGATCGGCGTCGACGCGATAGAAGACTACGCATTTGTGCGCGACTTCGTCGGCACGGTCGCC
TCGGCCGGCTGCGAAGTGTTCGTCGTGCATGCGCGCAACGCGATCCTGAAGGGGTTGTCGCCGAAGGAAAACCGCGAGAT
TCCGCCGCTCAAGTACGACTACGCGTATCGGCTCAAGCGCGACTTTCCGCAACTCGAAATCGTCATCAACGGCGGCATCA
CGACGCTCGACGAAGTCGAGCAGCATCTGAGGCACGTCGATGGCGTGATGCTCGGCCGCGAGGCATATCACAATCCGTAC
GTGCTCGCCGGCGTCGACGCCCGCTTCTACGGCGCGACCGAGCCGGCGCCGACGCGCGAGGCGGTGGAGGCGAAACTCGT
CGAATACTGCGCGGCCGAGCTCGCGCGCGGCACGTATCTCGGCGCGATCGTGCGGCATGCGCTCGGCCTGTATCGAGGCG
TCGCGGGTGCGCGCGGCTGGCGCCGCGTGCTGTCGGACAGCAAGCGTCTCGCGCGCGGCGATCTATCGATCTTCGATGAG
GCACGCGCGCACCTGACGAGCCCGGAAGAATTTTTTGAAAAAAAGGCTTTGCAAAGTTAA

Upstream 100 bases:

>100_bases
GCCAAGCCGGCGGCGGCACGCCCGGCCCGCGACGCCCGATGGCCGCCTTGCGGTAAAATGCCGGGTTGTCCACGCGCCCC
GGCGCTTTTCCGTACCGCCT

Downstream 100 bases:

>100_bases
ACAGCCTTTGTATAATCTCGCTTCTTCGTTGATGCCGCCGCCACGGCGAATCAAACGGAGAAAAATAGAGTGGTGGCTGT
AGCTCAGTTGGTAGAGTCCA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFTPDEAPVALQLGGSEPAELAH
SARLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHRIGVDAIEDYAFVRDFVGTVA
SAGCEVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQLEIVINGGITTLDEVEQHLRHVDGVMLGREAYHNPY
VLAGVDARFYGATEPAPTREAVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDSKRLARGDLSIFDE
ARAHLTSPEEFFEKKALQS

Sequences:

>Translated_339_residues
MSASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFTPDEAPVALQLGGSEPAELAH
SARLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHRIGVDAIEDYAFVRDFVGTVA
SAGCEVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQLEIVINGGITTLDEVEQHLRHVDGVMLGREAYHNPY
VLAGVDARFYGATEPAPTREAVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDSKRLARGDLSIFDE
ARAHLTSPEEFFEKKALQS
>Mature_338_residues
SASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFTPDEAPVALQLGGSEPAELAHS
ARLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHRIGVDAIEDYAFVRDFVGTVAS
AGCEVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQLEIVINGGITTLDEVEQHLRHVDGVMLGREAYHNPYV
LAGVDARFYGATEPAPTREAVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDSKRLARGDLSIFDEA
RAHLTSPEEFFEKKALQS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=237, Percent_Identity=29.957805907173, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40807366, Length=247, Percent_Identity=28.3400809716599, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=59.9358974358974, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788462, Length=287, Percent_Identity=27.8745644599303, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=237, Percent_Identity=24.8945147679325, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=29.7674418604651, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=24.896265560166, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24580595, Length=293, Percent_Identity=25.5972696245734, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920448, Length=293, Percent_Identity=25.5972696245734, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37510; Mature: 37379

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFT
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
PDEAPVALQLGGSEPAELAHSARLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCCCCHHHCCCCHHH
ADCVKAMRDAVSIPVTVKHRIGVDAIEDYAFVRDFVGTVASAGCEVFVVHARNAILKGLS
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCC
PKENREIPPLKYDYAYRLKRDFPQLEIVINGGITTLDEVEQHLRHVDGVMLGREAYHNPY
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
VLAGVDARFYGATEPAPTREAVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGW
EEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RRVLSDSKRLARGDLSIFDEARAHLTSPEEFFEKKALQS
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFT
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
PDEAPVALQLGGSEPAELAHSARLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCCCCHHHCCCCHHH
ADCVKAMRDAVSIPVTVKHRIGVDAIEDYAFVRDFVGTVASAGCEVFVVHARNAILKGLS
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCC
PKENREIPPLKYDYAYRLKRDFPQLEIVINGGITTLDEVEQHLRHVDGVMLGREAYHNPY
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
VLAGVDARFYGATEPAPTREAVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGW
EEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RRVLSDSKRLARGDLSIFDEARAHLTSPEEFFEKKALQS
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]