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The map label for this gene is tfdEI [H]

Identifier: 83721449

GI number: 83721449

Start: 3603697

End: 3604389

Strand: Direct

Name: tfdEI [H]

Synonym: BTH_I3160

Alternate gene names: 83721449

Gene position: 3603697-3604389 (Clockwise)

Preceding gene: 83721106

Following gene: 83719401

Centisome position: 94.61

GC content: 69.26

Gene sequence:

>693_bases
ATGGCTTCGCAATGGATCGACATCCCCGCAGGCAACGACACGTTCGGCGGCTACCTCGCGCTGCCCAAGCGCGGCAGGGG
CCCGGCCATCGTCATCATCCAGGAAATCTTCGGCGTGAACGGCCACATCCGCTCGGTCGCCGATCAATACGCGGCCGACG
GCTACGTCGCGCTCGCACCCGACGTGTTCTGGCGCACGCAGCCGCGCGTCGAGCTCGGCTATGAAGGCGCGGACCGCGAC
AAGGGCATCGAGCTGCTGCAGAAGACGGACGTCGTGCAGGCGGTCGCCGACATCGGCGCGGCAGCGGCGGCGCTGCGCGC
GCGCCCCGAGGTCGCGGGCAAGCTCGCCGCGATCGGCTATTGCTTCGGCGGGCGTCTCGCGTATCTCGCGGCGGCGCAGC
AGCACGTCGACGTCGCGGTCGCGTATTACGGCGGCGGCATCCAGAATCAAGTCGACGTCGCGGCGAAGGTCACGCAGCCG
ATCCTCTTCCACTACGCCGGCCACGACCAGTCGATTCCGCTCGACGCGGTCGACAAGGTGAAGGCCGCGTTCGCCGGCCG
CGCGAACGCCGAGTTCCACCTGTATCCGGACGCACAGCACGGCTTCAACTGCTCGGAGCGCGCATCGTACGACCAGCGCG
CGGCGGCGCTCGCGCACGGCCGCACGCTGACTTTCCTCGCCGAACGCCTGTAA

Upstream 100 bases:

>100_bases
TTCGCGGCGCTGCAGCGCCACGGGATTGATGGCACACTGGGCGCCGCTTCCACATTCACCGTCAGGACCTTCACCGGTCT
TGCTTCGCCAGGAGATCAGC

Downstream 100 bases:

>100_bases
GCTGACGCAATCGCCACGCCCGCGCACCGCGCGCGCCGTGTCACCGCTTTCGAATTCCGAAACGTTCGCCGCTCGTCACC
TCGGGGAGATGACTGTGCAG

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Carboxymethylenebutenolidase I; Dienelactone hydrolase I; DLH I [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MASQWIDIPAGNDTFGGYLALPKRGRGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAPDVFWRTQPRVELGYEGADRD
KGIELLQKTDVVQAVADIGAAAAALRARPEVAGKLAAIGYCFGGRLAYLAAAQQHVDVAVAYYGGGIQNQVDVAAKVTQP
ILFHYAGHDQSIPLDAVDKVKAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL

Sequences:

>Translated_230_residues
MASQWIDIPAGNDTFGGYLALPKRGRGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAPDVFWRTQPRVELGYEGADRD
KGIELLQKTDVVQAVADIGAAAAALRARPEVAGKLAAIGYCFGGRLAYLAAAQQHVDVAVAYYGGGIQNQVDVAAKVTQP
ILFHYAGHDQSIPLDAVDKVKAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL
>Mature_229_residues
ASQWIDIPAGNDTFGGYLALPKRGRGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAPDVFWRTQPRVELGYEGADRDK
GIELLQKTDVVQAVADIGAAAAALRARPEVAGKLAAIGYCFGGRLAYLAAAQQHVDVAVAYYGGGIQNQVDVAAKVTQPI
LFHYAGHDQSIPLDAVDKVKAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=234, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24594; Mature: 24463

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQWIDIPAGNDTFGGYLALPKRGRGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAP
CCCCEEECCCCCCCCCCEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCEEEECC
DVFWRTQPRVELGYEGADRDKGIELLQKTDVVQAVADIGAAAAALRARPEVAGKLAAIGY
CHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CFGGRLAYLAAAQQHVDVAVAYYGGGIQNQVDVAAKVTQPILFHYAGHDQSIPLDAVDKV
HHCCHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCCHHHHHHH
KAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL
HHHHCCCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
ASQWIDIPAGNDTFGGYLALPKRGRGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAP
CCCEEECCCCCCCCCCEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCEEEECC
DVFWRTQPRVELGYEGADRDKGIELLQKTDVVQAVADIGAAAAALRARPEVAGKLAAIGY
CHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CFGGRLAYLAAAQQHVDVAVAYYGGGIQNQVDVAAKVTQPILFHYAGHDQSIPLDAVDKV
HHCCHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCCHHHHHHH
KAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL
HHHHCCCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]