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The map label for this gene is ptsI [H]

Identifier: 83721348

GI number: 83721348

Start: 459002

End: 460774

Strand: Direct

Name: ptsI [H]

Synonym: BTH_I0413

Alternate gene names: 83721348

Gene position: 459002-460774 (Clockwise)

Preceding gene: 83718653

Following gene: 83720942

Centisome position: 12.05

GC content: 68.02

Gene sequence:

>1773_bases
GTGTCCTTCACGCTGCATGGCATTCCCGTTTCAAGAGGCATCGCGATCGGACGAGCGTATCTGATCGCGCCGGCGGCGCT
CGACGTCGCCCATTACCTGATCGAGGCCGAGCGCATCGAGGCCGAGATCGAACGCTTTCGCGCGGCGCTCGACGCGGTCC
GGCGCGAACTCGACGCGCTGCGCGCCGATCTGAGCGACGATACCCCGACCGAAGTCGCCGCATTCATCGACGTGCACGCG
ATGATCCTCGGCGACGCGATGCTCGTGCAGGAAACCATCGACCTGATCCGCACGCGCCGCTACAACGTCGAATGGGCGCT
GACCGAGCAGCTCGACGTGCTCGCCGGCCACTTCGACGACATCGAGGACGAGTACCTGCGCGAGCGGCGCGCGGACATCG
AGCAGGTCGTCGAACGGGTGCTGAAGGCGCTCGCGGGCGCGCCGTCCGCCGCGCAGGCGCTCGACCGCGCGGCGGGCAAC
GGCCGCGACGAGATGATCGTCGTCGCGCATGACATCGCGCCCGCCGACATGATGCAGTTCAAGACGCAGTCGTTCCAGGC
GTTCGTCACCGATCTGGGCGGGCGCACGTCGCACACGGCGATCGTCGCGCGCAGCCTCGGGATTCCGGCCGCGGTCGGCG
TCCAGCACGCGAGCGCGCTGATTCGTCAGGACGATCTCATCATCGTCGACGGCGATCAGGGCATCGTGATCGTCGATCCC
GCGCCGATCGTGCTCGAGGAATACTCGTACCGGCAATCGGAGAAGGCGCTCGAGCAGCGCAAGCTGCAGCGCCTGAAGTT
CTCGCCCGCGCAGACGCTCTGCGGCACGAAGATCGACCTGCTCGCGAACATCGAACTGCCTGACGACGCGAAGGCGGCCG
TCGACGCGGGCGCGGTCGGCGTCGGCCTGTTCCGCACCGAGTTCCTGTTCATGAGCAAGGAGCGGATGCCGGAGGAGGAG
GAGCAGTTCGCCGCGTACAAGCGCGCGGTCGAGCTGATGCACGGGATGCCGGTCACGATCCGCACGATCGACGTCGGCGC
GGACAAGCCGCTCGACGCGCACGACGAAGGCTACGAGACCGCGCCGAACCCGGCGCTCGGCCTGCGCGCGATCCGCTGGA
GCCTGTCGGAGCCGCAGATGTTCCTCACGCAGTTGCGCGCGATCCTGCGCGCGTCGGCGTTCGGCCAGGTGAAGATCCTG
GTGCCGATGCTCGCGCACGCGCAGGAGATCGACCAGACGCTCGACCTTATCAACGAGGCGAAGCGCCAGCTCGACGCGGC
GGGGCTCGCATACGATCCGAACGTGCGGGTTGGCGCGATGATCGAGATTCCGGCCGCGGCGATCGCGCTGCCGCTGTTCC
TGAAGCGCATCGATTTCCTGTCGATCGGCACGAACGACCTGATCCAGTACACGCTCGCGATCGACCGCGCGGACAACGCG
GTCGCGCATCTGTACGATCCGCTGCATCCGGCGGTGCTGCATCTGATCGCGTTCACGCTGCGCGAAGCGAAGCGCGCGGG
CGTGCCGGCGTCGGTGTGCGGCGAGATGGCGGGCGACCCGGCGCTGACGCGCCTGTTGCTGGGCATGGGGCTCACCGAGT
TCTCGATGCATCCGAGCCAGTTGCTCGTCGTCAAGCAGGAGATCCTGCGCGCACATTTGAAGGCGCTCGAGAAGCCGACC
GCGGACGTGCTCGCGGCGTTCGAGCCGGAAGAAGTGCAGGCGGCGCTCAAGCGGCTCGCGAGCGCGGAGCCGAAGGCGGA
CGTCGCCGCGTGA

Upstream 100 bases:

>100_bases
GCACGCAGTCACGCGGGGTATGCGGAATTTATAATCGACGATCGGGGTCATAAGCGCATCGCAGCGGTGCGCCGCGATTT
ACGTGATCAAGGAGGTGCGC

Downstream 100 bases:

>100_bases
TCGGGCGGGGCGCGCGTATGGGCGCGCGTGCCGTCTGCGGAATCCGGGGCGGCAGGAGCCGGGTATGGGGCACGCGCGAT
CGCGCGCCAGATTTTTGCGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRAALDAVRRELDALRADLSDDTPTEVAAFIDVHA
MILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDDIEDEYLRERRADIEQVVERVLKALAGAPSAAQALDRAAGN
GRDEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDP
APIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVGVGLFRTEFLFMSKERMPEEE
EQFAAYKRAVELMHGMPVTIRTIDVGADKPLDAHDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKIL
VPMLAHAQEIDQTLDLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRIDFLSIGTNDLIQYTLAIDRADNA
VAHLYDPLHPAVLHLIAFTLREAKRAGVPASVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEKPT
ADVLAAFEPEEVQAALKRLASAEPKADVAA

Sequences:

>Translated_590_residues
MSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRAALDAVRRELDALRADLSDDTPTEVAAFIDVHA
MILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDDIEDEYLRERRADIEQVVERVLKALAGAPSAAQALDRAAGN
GRDEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDP
APIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVGVGLFRTEFLFMSKERMPEEE
EQFAAYKRAVELMHGMPVTIRTIDVGADKPLDAHDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKIL
VPMLAHAQEIDQTLDLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRIDFLSIGTNDLIQYTLAIDRADNA
VAHLYDPLHPAVLHLIAFTLREAKRAGVPASVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEKPT
ADVLAAFEPEEVQAALKRLASAEPKADVAA
>Mature_589_residues
SFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRAALDAVRRELDALRADLSDDTPTEVAAFIDVHAM
ILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDDIEDEYLRERRADIEQVVERVLKALAGAPSAAQALDRAAGNG
RDEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDPA
PIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVGVGLFRTEFLFMSKERMPEEEE
QFAAYKRAVELMHGMPVTIRTIDVGADKPLDAHDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILV
PMLAHAQEIDQTLDLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRIDFLSIGTNDLIQYTLAIDRADNAV
AHLYDPLHPAVLHLIAFTLREAKRAGVPASVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEKPTA
DVLAAFEPEEVQAALKRLASAEPKADVAA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=568, Percent_Identity=38.2042253521127, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=34.2644320297952, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI48994992, Length=476, Percent_Identity=33.1932773109244, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788726, Length=593, Percent_Identity=27.4873524451939, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1787994, Length=416, Percent_Identity=27.6442307692308, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI226510935, Length=170, Percent_Identity=25.2941176470588, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64481; Mature: 64350

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRAALDAVRRELDAL
CCEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RADLSDDTPTEVAAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDD
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCC
IEDEYLRERRADIEQVVERVLKALAGAPSAAQALDRAAGNGRDEMIVVAHDIAPADMMQF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHH
KTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDP
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECC
APIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHHCCCHHH
VGLFRTEFLFMSKERMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDAHDEGYET
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCC
APNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILVPMLAHAQEIDQTLDLINEA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRIDFLSIGTNDLIQYTLAIDRADNA
HHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEHHEEECCCCH
VAHLYDPLHPAVLHLIAFTLREAKRAGVPASVCGEMAGDPALTRLLLGMGLTEFSMHPSQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHH
LLVVKQEILRAHLKALEKPTADVLAAFEPEEVQAALKRLASAEPKADVAA
HHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRAALDAVRRELDAL
CEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RADLSDDTPTEVAAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDD
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCC
IEDEYLRERRADIEQVVERVLKALAGAPSAAQALDRAAGNGRDEMIVVAHDIAPADMMQF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHH
KTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDP
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECC
APIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHHCCCHHH
VGLFRTEFLFMSKERMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDAHDEGYET
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCC
APNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILVPMLAHAQEIDQTLDLINEA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRIDFLSIGTNDLIQYTLAIDRADNA
HHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEHHEEECCCCH
VAHLYDPLHPAVLHLIAFTLREAKRAGVPASVCGEMAGDPALTRLLLGMGLTEFSMHPSQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHH
LLVVKQEILRAHLKALEKPTADVLAAFEPEEVQAALKRLASAEPKADVAA
HHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]