BacMap

Definition	Burkholderia thailandensis E264 chromosome chromosome I, complete sequence.
Accession	NC_007651
Length	3,809,201

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The map label for this gene is fadB1 [H]

Identifier: 83721196

GI number: 83721196

Start: 3333869

End: 3334645

Strand: Reverse

Name: fadB1 [H]

Synonym: BTH_I2899

Alternate gene names: 83721196

Gene position: 3334645-3333869 (Counterclockwise)

Preceding gene: 83718768

Following gene: 83720456

Centisome position: 87.54

GC content: 63.45

Gene sequence:

>777_bases
ATGGCTTACGAGAACATCCTGGTGGAGACGCGGGGCCGGGTGGGACTGATTACGCTGAATCGTCCGAAGGCGCTGAATGC
GCTGAACGACGCGCTGATGGACGAGCTCGGCGCGGCGCTCAAGGTATTTGATGCGGATGAGGGTATCGGCGCGATCGTGC
TGACGGGCAGCGAGAAGGCGTTCGCAGCTGGCGCCGACATCGGGATGATGGCGACCTATTCGTATATGGACGTCTTCAAG
GGCGACTACATCACGCGTAACTGGGAGGCGGTGCGTCAGGTTCGCAAGCCGGTCATTGCGGCGGTGGCGGGTTTCGCGCT
GGGCGGCGGCTGCGAGCTCGCGATGATGTGCGACATCATCTTTGCGGCCGATACCGCGAAATTCGGCCAACCCGAAATCA
AGCTCGGCGTGCTGCCGGGGGCGGGCGGCACGCAGCGTCTGCCGCGGGCGGTATCGAAGGCGAAGGCGATGGACATGTGC
CTAACCGCTCGCTTCATGGATGCGGAGGAGGCCGAGCGCGCGGGGCTGGTGTCGCGGATTCTGCCGGCCGACAAGTTGCT
CGACGAAGCGATTGCCGCGGCGTCGACGATCGCCGAGTTCTCGTTGCCCGCCGTGATGATGGTGAAGGAGGCGGTGAATC
GCGCGTACGAGACGACCTTGTCCGAAGGGGTGCACTTCGAGCGCCGCTTGTTTCATTCGGCGTTTGCAACTGAAGATCAG
AAGGAAGGCATGGCGGCGTTCGTCGAGAAGCGCAAGCCCGTCTTCAAGCACCGCTGA

Upstream 100 bases:

>100_bases
TGGCGGTCATTAATTAACCGACCGGTTGGTCGGAAAATGTTAGCATCAAATCGTCCGTACGTGTGCGGCATTTCATGTTT
CATTGATCGAGGAGAGGCAG

Downstream 100 bases:

>100_bases
ACGCGGCCAAAGTGTCGTGCGCCTTTGTTTTCTGCCGCGCGGCGCGTCTCGAATGAAAGCGCCTGCGCGCCTCGAGTTTC
TGACTTGCCGCGTGCCTGAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKVFDADEGIGAIVLTGSEKAFAAGADIGMMATYSYMDVFK
GDYITRNWEAVRQVRKPVIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAEEAERAGLVSRILPADKLLDEAIAAASTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
KEGMAAFVEKRKPVFKHR

Sequences:

>Translated_258_residues
MAYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKVFDADEGIGAIVLTGSEKAFAAGADIGMMATYSYMDVFK
GDYITRNWEAVRQVRKPVIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAEEAERAGLVSRILPADKLLDEAIAAASTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
KEGMAAFVEKRKPVFKHR
>Mature_257_residues
AYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKVFDADEGIGAIVLTGSEKAFAAGADIGMMATYSYMDVFKG
DYITRNWEAVRQVRKPVIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMCL
TARFMDAEEAERAGLVSRILPADKLLDEAIAAASTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQK
EGMAAFVEKRKPVFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=57.5289575289575, Blast_Score=297, Evalue=6e-81,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=35.9375, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=29.6747967479675, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI68989263, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=31.4606741573034, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=35.0282485875706, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=32.7868852459016, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=214, Percent_Identity=30.3738317757009, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI37594469, Length=214, Percent_Identity=30.3738317757009, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=27.9279279279279, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=27.9279279279279, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=27.9279279279279, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=23.2824427480916, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=23.2824427480916, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=22.9885057471264, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=22.9885057471264, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=221, Percent_Identity=27.6018099547511, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=221, Percent_Identity=27.6018099547511, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=221, Percent_Identity=27.6018099547511, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=25.1184834123223, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=25.1184834123223, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=32.5925925925926, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=225, Percent_Identity=24.8888888888889, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=44.6640316205534, Blast_Score=213, Evalue=8e-57,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.5384615384615, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=34.0996168582375, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=35.1351351351351, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=31.6981132075472, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=31.8181818181818, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=30.9782608695652, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=58.0769230769231, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=54.320987654321, Blast_Score=266, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=34.251968503937, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=33.195020746888, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.3636363636364, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=35.7142857142857, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=32.2751322751323, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.953125, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=28.9473684210526, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=33.5195530726257, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=220, Percent_Identity=29.0909090909091, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=220, Percent_Identity=29.0909090909091, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=24.7058823529412, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=27.4611398963731, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=58.5271317829457, Blast_Score=304, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=58.5271317829457, Blast_Score=304, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=37.9699248120301, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=32.3076923076923, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24650670, Length=256, Percent_Identity=30.859375, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=32.5490196078431, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=28.0632411067194, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=31.5508021390374, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19921000, Length=170, Percent_Identity=34.7058823529412, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571729, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27859; Mature: 27727

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKVFDADEGIGAIVLTGSEKA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVFKGDYITRNWEAVRQVRKPVIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMCLTARFMDAEEAERAGLVSRI
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LPADKLLDEAIAAASTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKVFDADEGIGAIVLTGSEKA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVFKGDYITRNWEAVRQVRKPVIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMCLTARFMDAEEAERAGLVSRI
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LPADKLLDEAIAAASTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]