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The map label for this gene is purR [H]

Identifier: 83721164

GI number: 83721164

Start: 332040

End: 333059

Strand: Reverse

Name: purR [H]

Synonym: BTH_I0300

Alternate gene names: 83721164

Gene position: 333059-332040 (Counterclockwise)

Preceding gene: 83718421

Following gene: 83720329

Centisome position: 8.74

GC content: 71.18

Gene sequence:

>1020_bases
ATGGGCACCACCATTCGCGACGTTGCGCAAGCGGCCAACGTGTCGATCGGCACCGTGTCGCGCGCGCTCAAGAATCAGCC
CGGCCTGTCCGAGGCGACGCGCGCGCGCATCGTCGAGATCGCGCATCGGATGAACTACGATCCGACGCAACTGCGCCCGC
GGATCAAGCGGCTCACGTTCCTGCTGCATCGCCAGCACAACAACTTCGCGACGACGCCGTTCTTCTCCCACGTGCTGCAC
GGCGTGGAAGATGCATGCCGCGAGCGCGGCATCGTGCCGTCGCTGCTGACGACGGGCCCGACCGACGACGTGATCCGCCA
GATGCGTCCGCACGCGCCCGACGCGATCGCGGTCGCGGGTTTCATGGAGCCGGAGACGCTCGAGGCGCTCGCCGCGACGG
GCCGGCCGCTCGTGCTCATCGACATCCGCGCGCCCGGACTGCGCTCGATCAACGTCGACAACGCGGGCGGCGCGGCGCTC
GCGATGCATCACCTGTTCGCATCGGGCCGCCGGCGCATCGCGTTCATCGGCGGCTCGCTCGCGCACTACAGCATCGCGCA
GCGCGCGCTCGGCTATCGCCGTGCGTTCTTCGACGCGGGCATGCTGTTCGATCCGTCGCTCGAGCTGTCGATCGACACGA
GCCTCGGCCCCGACGCCGGCGCGGCGCACGCGATGCGGCAACTGCTCGACGCGGGCGGCCCGCTGCCCGACGCAGTGTTC
GCGTACAACGATGCGGCCGCACTCGCCGCGATGCGCGTGTGCGTCGAGCGCGGCGTGCGCGTGCCCGAGGACATCGCATT
CGTCGGCTTCGACGACATCCCGGCCGCCGCGCACGCGGCCCCGCCCCTCACGACGCTCGCGATCGACAAGGAAGCGCTCG
GCCGGCAAGGCGTCGAGCTGCTGCTCGCCGATTCGCCCGCGCAAGCCGAACTCAGCCTCCCCGTGCAGCTCGTCGCGCGG
GCCAGCAGCGCAGGCGCCGCACACGCGCCGCGCGCCCTCACCTCGGTACTCGGATCATGA

Upstream 100 bases:

>100_bases
ATACATAAGCACTTATAAGCGCGATCGAGATGATTCCGGGACGTTTCGCGGCGATTTTTACTATACAATCGCCAACACCC
TAAACATCCCGGCTCAAACG

Downstream 100 bases:

>100_bases
CGACCTCAGCCACGCCCGCCCCCCGTCCGGCGCCCAACGCGCCGCCCGCCGCCGCGCCGTACGTCGCGAGCTTCCGCGAC
CCGCAGTTCCTCGTCGCGCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTFLLHRQHNNFATTPFFSHVLH
GVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAGFMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAAL
AMHHLFASGRRRIAFIGGSLAHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAAHAMRQLLDAGGPLPDAVF
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVELLLADSPAQAELSLPVQLVAR
ASSAGAAHAPRALTSVLGS

Sequences:

>Translated_339_residues
MGTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTFLLHRQHNNFATTPFFSHVLH
GVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAGFMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAAL
AMHHLFASGRRRIAFIGGSLAHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAAHAMRQLLDAGGPLPDAVF
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVELLLADSPAQAELSLPVQLVAR
ASSAGAAHAPRALTSVLGS
>Mature_338_residues
GTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTFLLHRQHNNFATTPFFSHVLHG
VEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAGFMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAALA
MHHLFASGRRRIAFIGGSLAHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAAHAMRQLLDAGGPLPDAVFA
YNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVELLLADSPAQAELSLPVQLVARA
SSAGAAHAPRALTSVLGS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=324, Percent_Identity=32.4074074074074, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=32.484076433121, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=28.2131661442006, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=31.0580204778157, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787948, Length=315, Percent_Identity=28.2539682539683, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786540, Length=370, Percent_Identity=28.1081081081081, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=25.9375, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788474, Length=329, Percent_Identity=27.355623100304, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=27.4193548387097, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=27.3615635179153, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=25.4716981132075, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35909; Mature: 35777

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTF
CCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLHRQHNNFATTPFFSHVLHGVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCCEEEEC
FMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAALAMHHLFASGRRRIAFIGGSL
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAAHAMRQLLDAGGPLPDAVF
HHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHE
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVEL
ECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCEEEEECHHHHCCCCCEE
LLADSPAQAELSLPVQLVARASSAGAAHAPRALTSVLGS
EEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
GTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVEIAHRMNYDPTQLRPRIKRLTF
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLHRQHNNFATTPFFSHVLHGVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCCEEEEC
FMEPETLEALAATGRPLVLIDIRAPGLRSINVDNAGGAALAMHHLFASGRRRIAFIGGSL
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRALGYRRAFFDAGMLFDPSLELSIDTSLGPDAGAAHAMRQLLDAGGPLPDAVF
HHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHE
AYNDAAALAAMRVCVERGVRVPEDIAFVGFDDIPAAAHAAPPLTTLAIDKEALGRQGVEL
ECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCEEEEECHHHHCCCCCEE
LLADSPAQAELSLPVQLVARASSAGAAHAPRALTSVLGS
EEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA