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The map label for this gene is maiA [H]

Identifier: 83721103

GI number: 83721103

Start: 520683

End: 521327

Strand: Direct

Name: maiA [H]

Synonym: BTH_I0465

Alternate gene names: 83721103

Gene position: 520683-521327 (Clockwise)

Preceding gene: 83719396

Following gene: 83719191

Centisome position: 13.67

GC content: 64.5

Gene sequence:

>645_bases
ATGAAGCTATATAGCTATTTCCGCAGTTCGGCGGCATTTCGCGTGCGCATCGCGCTGCATCTGAAGAAGCTGCCGTTCGA
CTACGTGCCCGTGCATCTGCTGCGCGACGGCGGCCAGCAGTTGACGGACGACTACCGCGCGCTCAACCCCGATGCGCTCG
TGCCGACGCTCATCGACGGCGATTCGCCGTTGCAGCAGTCGCTCGCGATCGTCGAATACCTCGATGAAGCGTATCCGGCC
GTGCCGTTGTTGCCGAAGGCGCCCGTCGATCGCGCGTACGTGCGCGCGATCGCGCTGCAGATTGCCTGCGAGATCCATCC
GCTCAACAACCTGCGCGTGCTGAAGTACCTGAAGCACACGCTGCAGGTCGACGACGACGCGAAGGACGCGTGGTACCGCC
ACTGGATCGAGGACGGCTTCAAGTCGCTCGAGGCGCGTCTGTCGAGCGATCCGCGCACGGGCAAGCTGTGCTTCGGCGAT
ACGCCGACGCTCGCCGATCTGTGCATCGTGCCGCAGGTGTTCAACGCGAAGCGCTTCTCGATCGGCCTCGAGCGGTTTCC
GACGATTCAGCGCATCCACGATCATGCGATGACGATCGACGCGTTCAAGGCGGCCGCGCCCGCCGCGCAGCCGGACGCCG
AGTGA

Upstream 100 bases:

>100_bases
GAAGCGCTCGGACGCCCGCCTGCCGGCTTCGGGGCGCGCGTCTGCCGTCGCGCCCGAACGCCGGCGAGAAGATCGACGAT
CGAGACAATGAGGGACACCG

Downstream 100 bases:

>100_bases
GCGCTGCGTCGGCCGCGCGCGGATGAAGAACGGGCGCCTTGCCGAGCCGCGCCGTCGACGGCCGGACATCGAGTCCGGCG
TTTGACGCGCGGTTCGCGCA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLIDGDSPLQQSLAIVEYLDEAYPA
VPLLPKAPVDRAYVRAIALQIACEIHPLNNLRVLKYLKHTLQVDDDAKDAWYRHWIEDGFKSLEARLSSDPRTGKLCFGD
TPTLADLCIVPQVFNAKRFSIGLERFPTIQRIHDHAMTIDAFKAAAPAAQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLIDGDSPLQQSLAIVEYLDEAYPA
VPLLPKAPVDRAYVRAIALQIACEIHPLNNLRVLKYLKHTLQVDDDAKDAWYRHWIEDGFKSLEARLSSDPRTGKLCFGD
TPTLADLCIVPQVFNAKRFSIGLERFPTIQRIHDHAMTIDAFKAAAPAAQPDAE
>Mature_214_residues
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLIDGDSPLQQSLAIVEYLDEAYPA
VPLLPKAPVDRAYVRAIALQIACEIHPLNNLRVLKYLKHTLQVDDDAKDAWYRHWIEDGFKSLEARLSSDPRTGKLCFGD
TPTLADLCIVPQVFNAKRFSIGLERFPTIQRIHDHAMTIDAFKAAAPAAQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=48.5849056603774, Blast_Score=188, Evalue=2e-48,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=35.8490566037736, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI22202622, Length=161, Percent_Identity=45.9627329192547, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=41.2322274881517, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=41.2322274881517, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=38.6792452830189, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21355859, Length=212, Percent_Identity=45.7547169811321, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI45553325, Length=212, Percent_Identity=45.7547169811321, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24645375, Length=212, Percent_Identity=45.7547169811321, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI21355857, Length=213, Percent_Identity=43.6619718309859, Blast_Score=167, Evalue=4e-42,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24124; Mature: 24124

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLIDG
CCHHHHHHCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEECC
DSPLQQSLAIVEYLDEAYPAVPLLPKAPVDRAYVRAIALQIACEIHPLNNLRVLKYLKHT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LQVDDDAKDAWYRHWIEDGFKSLEARLSSDPRTGKLCFGDTPTLADLCIVPQVFNAKRFS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCCHHHH
IGLERFPTIQRIHDHAMTIDAFKAAAPAAQPDAE
HCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLIDG
CCHHHHHHCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEECC
DSPLQQSLAIVEYLDEAYPAVPLLPKAPVDRAYVRAIALQIACEIHPLNNLRVLKYLKHT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LQVDDDAKDAWYRHWIEDGFKSLEARLSSDPRTGKLCFGDTPTLADLCIVPQVFNAKRFS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCCHHHH
IGLERFPTIQRIHDHAMTIDAFKAAAPAAQPDAE
HCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]